Role of elderberry (<i>Sambucus nigra)</i> on the modulation of stress and immune response of Nile tilapia, <i>Oreochromis niloticus</i>

Khan, Mursalin; Mortuza, Asif; Blumenthal, Elliott; Mustafa, Ahmed

doi:10.1080/10454438.2022.2026269

Cited by 5 publications

(5 citation statements)

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“…immune-modulating components of the EB extract are anthocyanins, phenolic acids, and flavonoids [40,56]. Although the immune-stimulating role of these secondary metabolites is well established in the literature, immunological studies relating to fish immune cells are limited to our knowledge [14,37,[57][58][59]. In addition, most of the experiments performed to understand the role of the nutraceuticals were performed in the in vivo condition, which does not provide specific information regarding the capabilities of the nutraceuticals at the cellular level.…”

Section: Plos Onementioning

confidence: 99%

“…Due to the wide range of health benefits that can be achieved using various berries such as elderberry (EB), it is important to assess their effect on various species, for instance, fish and shellfish. However, there is very little is known about the effect of elderberry on fish models [14]. In our study, we are using one of the most cultivated aquaculture fish, Nile tilapia, to decipher the role of elderberry extract on the fish's immune system.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of the role of anthocyanin standardized elderberry (Sambucus nigra) extract as an immune-stimulating nutraceutical of Nile tilapia, Oreochromis niloticus

et al. 2022

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The study of nutraceuticals and their connection to immunity is an expanding field of research. The use of nutraceuticals to alleviate stress and enhance immunity in adverse aquaculture environments have been examined to a certain extent. To elucidate the understanding, we focused on the immunological effect of membrane-separated 13% anthocyanin standardized elderberry (EB) extract with maltodextrin excipient, widely used first-line nutraceuticals to augment the immunity, in aquaculture fish, Nile tilapia. To evaluate the potential of EB-extract, we assessed their capability to enhance lymphocyte proliferation and interleukin-2 production in an in-vitro condition using spleen and thymus lymphocytes. The experiments on spleen and thymus T-cells demonstrated significantly higher T-cell proliferation by EB-extract when lectin mitogen Con A was present as a stimulator. Likewise, our spleen B-cell proliferation result reveals a significant effect of EB-extracts, along with B-cell stimulator non-lectin mitogen LPS. Further, the quantification of IL-2 indicates elevated IL-2 levels when spleen T-cells were cultured with EB-extracts and with Con A present as a stimulator. These suggest that 13% anthocyanin standardized EB-extracts can aggrandize fish cells’ cellular and humoral immune responses. With further research, elderberry extracts could be used to supplement commercial feed in aquaculture to reduce stress and stimulate the immune response.

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Section: Plos Onementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessment of the role of anthocyanin standardized elderberry (Sambucus nigra) extract as an immune-stimulating nutraceutical of Nile tilapia, Oreochromis niloticus

et al. 2022

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“…The results of the study (Table 1) show that when 10 % of the derivative product obtained by osmotic dehydration of elderberry fruits is added to sugar, it acquires certain antioxidant properties. The use of osmotic dehydration for the treatment of elder fruits allows you to save vitamin C and flavonoids, which are contained in large quantities in it [2,12]. Quercetin (a flavonoid) that enters the composition of sugar with an elderberry derivative is considered a natural pigment.…”

Section: Discussion Of the Results Of The Study Of The Effect Of Deri...mentioning

confidence: 99%

“…Elderberry fruits also contain ascorbic acid (6-25 mg/100 g), and elderberry seed flour is a source of vitamin E and gamma-tocopherol [11]. According to other data, the concentration of vitamin C was found in the amount of 32.75-44.42 mg/100g [12]. The main sugars identified in elderberries are glucose (33.33-50.23 g/kg) and fructose (33.99-52.25 g/kg).…”

Section: Introductionmentioning

confidence: 99%

Determination of quality indicators of sugar fortified with a by-product of elderberry processing

Samilyk,

Bal’-Prylipko,

Korniienko

et al. 2023

EEJET

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Granulated and pressed beet sugar consists of 99.61–99.7 % sucrose, which is a source of energy for the body. However, this product does not contain other vital nutrients. The object of the study is the method of non-waste processing of elderberry fruits. Wild-growing fruits of black elder Sambucus nigra L were used as the subject of the study. Pre-cleaned elder fruits were frozen at a temperature of –18±2 °C, and after defrosting they were subjected to osmotic dehydration. For this, a 70 % sugar solution with a temperature of 50±5 °C was used (hydromodule 1). The duration of osmosis was 1 hour. The derivative product formed as a result of osmotic dehydration of elderberries (elderberry syrup) was used to enrich granulated sugar in an amount of 10 % by weight of sugar. After thorough mixing with the solution, the sugar was dried in a laboratory vacuum dryer. Anthocyanin dyes contained in the elderberries gave the sugar a bright pink color. The resulting product had a characteristic smell and taste of elderberry. The composition of sugar was studied by high-performance liquid chromatography. It was found that sugar enriched with an elderberry derivative contains 0.03±0.02 mg/100 g of vitamin C and 0.28±0.02 % flavonoids. This gives it certain antioxidant properties. In addition to sucrose, glucose (0.20±0.02) and fructose (0.27±0.02) were found in the product by the polarimetric method. Analysis of the amino acid spectrum of enriched sugar showed the presence of 18 amino acids (total amount of 5.547 mg/100 g), including all essential ones. The most found in enriched sugar, mg/100g: tyrosine (0.93), alanine (0.79), phenylalanine (0.752) and leucine (0.749). The results obtained indicate an increase in the biological value and additional functional properties of fortified sugar

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“…In this study, dietary BA supplementation decreased the expression of the above nine immune-related DEGs and the corresponding signaling pathways. Anthocyanin regulated the innate immune system and reduced bacterial systemic dissemination in mice challenged with Klebsiella pneumoniae (Dong et al, 2021 ) and was used as a nutraceutical strategy to modulate stress and immune response in vertebrates (Khan et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and proteome profile of jejunum in chickens challenged with Salmonella Typhimurium revealed the effects of dietary bilberry anthocyanin on immune function

Zhang,

Wang,

et al. 2023

Front. Microbiol.

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IntroductionThe present study investigated the effects of bilberry anthocyanin (BA) on immune function when alleviating Salmonella Typhimurium (S. Typhimurium) infection in chickens.MethodsA total of 180 newly hatched yellow-feathered male chicks were assigned to three groups (CON, SI, and SI + BA). Birds in CON and SI were fed a basal diet, and those in SI + BA were supplemented with 100 mg/kg BA for 18 days. Birds in SI and SI + BA received 0.5 ml suspension of S. Typhimurium (2 × 109 CFU/ml) by oral gavage at 14 and 16 days of age, and those in CON received equal volumes of sterile PBS.ResultsAt day 18, (1) dietary BA alleviated weight loss of chickens caused by S. Typhimurium infection (P < 0.01). (2) Supplementation with BA reduced the relative weight of the bursa of Fabricius (P < 0.01) and jejunal villus height (P < 0.05) and increased the number of goblet cells (P < 0.01) and the expression of MUC2 (P < 0.05) in jejunal mucosa, compared with birds in SI. (3) Supplementation with BA decreased (P < 0.05) the concentration of immunoglobulins and cytokines in plasma (IgA, IL-1β, IL-8, and IFN-β) and jejunal mucosa (IgG, IgM, sIgA, IL-1β, IL-6, IL-8, TNF-α, IFN-β, and IFN-γ) of S. Typhimurium-infected chickens. (4) BA regulated a variety of biological processes, especially the defense response to bacteria and humoral immune response, and suppressed cytokine–cytokine receptor interaction and intestinal immune network for IgA production pathways by downregulating 6 immune-related proteins.ConclusionIn summary, the impaired growth performance and disruption of jejunal morphology caused by S. Typhimurium were alleviated by dietary BA by affecting the expression of immune-related genes and proteins, and signaling pathways are related to immune response associated with immune cytokine receptors and production in jejunum.

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Role of elderberry (Sambucus nigra) on the modulation of stress and immune response of Nile tilapia, Oreochromis niloticus

Cited by 5 publications

References 81 publications

Assessment of the role of anthocyanin standardized elderberry (Sambucus nigra) extract as an immune-stimulating nutraceutical of Nile tilapia, Oreochromis niloticus

Assessment of the role of anthocyanin standardized elderberry (Sambucus nigra) extract as an immune-stimulating nutraceutical of Nile tilapia, Oreochromis niloticus

Determination of quality indicators of sugar fortified with a by-product of elderberry processing

Transcriptome and proteome profile of jejunum in chickens challenged with Salmonella Typhimurium revealed the effects of dietary bilberry anthocyanin on immune function

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