Role of electrostatic interactions in the toxicity of titanium dioxide nanoparticles toward Escherichia coli

Pagnout, Christophe; Jomini, Stéphane; Dadhwal, Mandeep; Caillet, Céline; Thomas, Fabien; Bauda, Pascale

doi:10.1016/j.colsurfb.2011.12.012

Cited by 101 publications

(92 citation statements)

References 28 publications

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“…Little published literature has also confirmed this finding which indicates that there are additional mechanisms responsible for the toxicity (Pagnout et al 2012;Zhukova et al 2012). Adams et al (2006) showed inhibition of Escherichia coli and Bacillus subtilis by TiO 2 in dark.…”

Section: Introductionmentioning

confidence: 57%

Inhibition of bacteria by photocatalytic nano-TiO2 particles in the absence of light

Erdem

Metzler

Cha

et al. 2014

Int. J. Environ. Sci. Technol.

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The potential eco-toxicity of fourteen different nanosized titanium dioxide (TiO 2 ) particles was studied using Gram-positive Bacillus subtilis and Gram-negative Escherichia coli (ATCC K12) as test organisms. These photosensitive nanoparticles (NPs) were found to be harmful to the organisms studied at different degrees; the antibacterial activity increased with primary particle size, reached the maximum level in the range of 16-20 nm, and then decreased as the primary particle size increased. The presence of light played a significant role on the eco-toxicity of the nano-TiO 2 particles under most conditions studied, presumably due to the generation of reactive oxygen species (ROS). However, bacterial growth was inhibited also under dark condition, indicating that mechanisms other than photocatalytic ROS were responsible for the toxic effect. Results highlight the need for caution during the use and disposal of manufactured NPs as to prevent unintended environmental impacts, as well as the importance of further research on the mechanisms and factors that control the toxicity of NPs toward aquatic organisms.

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Section: Introductionmentioning

confidence: 57%

Inhibition of bacteria by photocatalytic nano-TiO2 particles in the absence of light

Erdem

Metzler

Cha

et al. 2014

Int. J. Environ. Sci. Technol.

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“…Microorganisms play an important role on the functioning of any ecosystem. Because of being primary agents of organic matter transformation, regeneration of nutrients, and as a food source for higher trophic levels, microorganisms are a great concern for toxicity studies (Foster et al 2011;Pagnout et al 2012). Several studies and reviews have been reported which aimed to determine the toxicity of nano-TiO 2 on different organisms such as microorganisms (Coleman et al 2005;Huang et al 2000;Simon-Deckers et al 2009;von Moos and Slaveykova 2014;Wei et al 1994) and algae (Clément et al 2013;Metzler et al 2011Metzler et al , 2012.…”

Section: Introductionmentioning

confidence: 98%

The short-term toxic effects of TiO2 nanoparticles toward bacteria through viability, cellular respiration, and lipid peroxidation

Erdem

Metzler

Daniel

et al. 2015

Environ Sci Pollut Res

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To better understand the potential impacts of metal oxide nanoparticles (NPs) on Gram(+) Bacillus subtilis and Gram(-) Escherichia coli (K12) bacteria, eight different nanosized titanium dioxide (TiO2) suspensions with five different concentrations were used. Water quality parameters (pH, temperature, and ionic strength), light sources, and light intensities were also changed to achieve different environmental conditions. The photosensitive TiO2 NPs were found to be harmful to varying degrees under ambient conditions, with antibacterial activity increasing with primary particle sizes from 16 to 20 nm. The presence of light was a significant factor under most conditions tested, presumably due to its role in promoting generation of reactive oxygen species (ROS). However, bacterial growth inhibition was also observed under dark conditions and different water quality parameters, indicating that undetermined mechanisms additional to photocatalytic ROS production were responsible for toxicity. The results also indicated that nano-TiO2 particles in the absence and the presence of photoactivation induced lipid peroxidation and cellular respiration disruption.

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“…We previously highlighted the paramount role that electrostatic forces play in the interactions between NPs and bacteria, and the importance of medium characteristics such as pH, ionic strength, and electrolyte composition in controlling these forces [ 37 ]. We also examined the behavior of TiO 2 -NPs in complex medium, such as the test medium of the Bacterial Reverse Mutation assay, and revealed their immediate and rapid aggregation as soon as they are introduced [ 38 ].…”

Section: Np Genotoxicity and Evaluation Using The Bacterial Reverse Mmentioning

confidence: 97%

Revised Procedure of the Bacterial Reverse Mutation Test for Genotoxic Evaluation of Nanoparticles

Pagnout

Jomini

Bauda

2014

Genotoxicity and DNA Repair

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A recent review of in vitro genotoxicity testing strategies for nanoparticles (NPs) revealed that the conventional Bacterial Reverse Mutation Test (Ames test) primarily yielded negative results, whereas the other in vitro genotoxicity assays were usually positive. Accordingly, the effi ciency of the test for NP evaluation was questioned, as was the NP entrance in bacterial cells. Indeed, prokaryotes are unable to perform endocytosis and NPs are too large to be transported through the pores of the cell wall. However, regardless of whether they have been internalized, the mutagenic potential of free radicals, produced intrinsically or indirectly by NPs adsorbed onto the bacterial cell walls, should be detected by the Bacterial Reverse Mutation Test .Another phenomenon that can explain the low test effi ciency is the lack of interactions between NPs and cells due to strong NP aggregation in the exposure medium of the assay and the presence of electrostatic repulsive forces between NPs and bacteria, which both carry overall negative charge. This hypothesis is supported by our recent study that revealed the mutagenic potential of NP-TiO 2 using a revised procedure of the Bacterial Reverse Mutation Test , which improves the NP-cell interactions and the sensitivity of the test.In this chapter, we provide several recommendations for the genotoxic evaluation of NPs and propose a revised version of the Bacterial Reverse Mutation Test more in line with the specifi c properties of NPs.

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Role of electrostatic interactions in the toxicity of titanium dioxide nanoparticles toward Escherichia coli

Cited by 101 publications

References 28 publications

Inhibition of bacteria by photocatalytic nano-TiO2 particles in the absence of light

Inhibition of bacteria by photocatalytic nano-TiO2 particles in the absence of light

The short-term toxic effects of TiO2 nanoparticles toward bacteria through viability, cellular respiration, and lipid peroxidation

Revised Procedure of the Bacterial Reverse Mutation Test for Genotoxic Evaluation of Nanoparticles

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