Role of electrostatic repulsion on colloidal stability of Bacillus halmapalus alpha-amylase

Olsen, Søren N.; Andersen, Kim B.; Randolph, Ted; Carpenter, John F.; Westh, Peter

doi:10.1016/j.bbapap.2009.02.010

Cited by 47 publications

(41 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have largely succeeded in the first task. We have confirmed previous studies indicating that native-state electrostatic interactions correlate with aggregate growth pathways [9,43,71,75] and growth rates [5,19,63], although, we have highlighted a protein, the 5R mutant, where the native-state electrostatic interactions are not apparent from the value of k D . As such a better indicator of electrostatic interactions might be a direct quantification of the protein electrostatic properties either by theoretical calculations or by experimental verification using electrophoretic measurements.…”

Section: Discussionsupporting

confidence: 75%

“…A non-specific increase in aggregate growth rates occurs when the repulsive double-layer forces are reduced either by changing pH to reduce protein net charge or by electrostatic screening through increasing in the ionic strength [5,43,47,60,61,63,66]. The electrostatic interactions are greater for the scFv at pH 5 versus pH 7 due the larger net charge (see Table 1 for theoretical estimation of net charge).…”

Section: ⁄ Reflect Relative Aggregate Growth Ratesmentioning

confidence: 99%

“…The ionic strength and pH trends of T SLS ⁄ reflect the expected changes in aggregate growth rates due to the impact of electrostatic interactions, which has been rationalized using DLVO theory [5,19,63]. A non-specific increase in aggregate growth rates occurs when the repulsive double-layer forces are reduced either by changing pH to reduce protein net charge or by electrostatic screening through increasing in the ionic strength [5,43,47,60,61,63,66].…”

Section: ⁄ Reflect Relative Aggregate Growth Ratesmentioning

confidence: 99%

“…A decreasing k D value is often correlated with increased aggregation propensity when reducing electrostatic repulsion by increasing ionic strength or using a salt or buffer ion that neutralizes protein charge [35,46,57,74,78,82,19,46,63,77,81]. Consistent with these studies, for the wild-type, SB and DSV mutants, the relative rates of aggregate growth (as reflected by the corresponding T SLS ⁄ values) and protein-protein interactions (as reflected by the k D values) decrease non-specifically upon addition of NaCl due to screening of electrostatic interactions.…”

Section: Correlations Between K D and Aggregation Propensitymentioning

confidence: 99%

“…For instance, recent work has established a strong correlation between the electrostatic contribution to native protein-protein interactions and the type of aggregate growth pathways as well as aggregate growth rates [9,43,63,71,75]. Electrostatic interactions correlate with aggregate growth because charged groups remain exposed in the native state and on growing aggregates.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

The effect of charge mutations on the stability and aggregation of a human single chain Fv fragment

Austerberry

Dajani

Panova

et al. 2017

European Journal of Pharmaceutics and Biopharmaceutics

View full text Add to dashboard Cite

a b s t r a c tThe aggregation propensities for a series of single-chain variable fragment (scFv) mutant proteins containing supercharged sequences, salt bridges and lysine/arginine-enriched motifs were characterised as a function of pH and ionic strength to isolate the electrostatic contributions. Recent improvements in aggregation predictors rely on using knowledge of native-state protein-protein interactions. Consistent with previous findings, electrostatic contributions to native protein-protein interactions correlate with aggregate growth pathway and rates. However, strong reversible self-association observed for selected mutants under native conditions did not correlate with aggregate growth, indicating 'sticky' surfaces that are exposed in the native monomeric state are inaccessible when aggregates grow. We find that even though similar native-state protein-protein interactions occur for the arginine and lysine-enriched mutants, aggregation propensity is increased for the former and decreased for the latter, providing evidence that lysine suppresses interactions between partially folded states under these conditions. The supercharged mutants follow the behaviour observed for basic proteins under acidic conditions; where excess net charge decreases conformational stability and increases nucleation rates, but conversely reduces aggregate growth rates due to increased intermolecular electrostatic repulsion. The results highlight the limitations of using conformational stability and native-state protein-protein interactions as predictors for aggregation propensity and provide guidance on how to engineer stabilizing charged mutations.

show abstract

Section: Discussionsupporting

confidence: 75%

Section: ⁄ Reflect Relative Aggregate Growth Ratesmentioning

confidence: 99%

Section: ⁄ Reflect Relative Aggregate Growth Ratesmentioning

confidence: 99%

Section: Correlations Between K D and Aggregation Propensitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The effect of charge mutations on the stability and aggregation of a human single chain Fv fragment

Austerberry

Dajani

Panova

et al. 2017

European Journal of Pharmaceutics and Biopharmaceutics

View full text Add to dashboard Cite

show abstract

Molecular Modeling for Protein Aggregation and Formulation

Roberts

Warwicker

Curtis

2015

Computational Pharmaceutics

View full text Add to dashboard Cite

Electrostatic‐Assisted Liquefaction of Porous Carbons

Schott

Zhang

et al. 2017

Angewandte Chemie

View full text Add to dashboard Cite

Porous liquids are an ewly developed porous material that combine unique fluidity with permanent porosity, which exhibit promising functionalities for av ariety of applications.H owever,t he apparent incompatibility between fluidity and permanent porosity makes the stabilization of porous nanoparticle with still empty pores in the dense liquid phase as ignificant challenging.H erein, by exploiting the electrostatic interaction between carbon networks and polymerized ionic liquids,w ed emonstrate that carbon-based porous nanoarchitectures can be well stabilized in liquids to affordpermanent porosity,and thus opens up anew approach to prepare porous carbon liquids.F urthermore,w eh ope this facile synthesis strategy can be widely applicated to fabricate other types of porous liquids,s uch as those (e.g., carbon nitride,b oron nitride,m etal-organic frameworks,c ovalent organic frameworks etc.) also having the electrostatic interaction with polymerizedionic liquids,evidently advancing the development and understanding of porous liquids.

show abstract

Role of electrostatic repulsion on colloidal stability of Bacillus halmapalus alpha-amylase

Cited by 47 publications

References 51 publications

The effect of charge mutations on the stability and aggregation of a human single chain Fv fragment

The effect of charge mutations on the stability and aggregation of a human single chain Fv fragment

Molecular Modeling for Protein Aggregation and Formulation

Electrostatic‐Assisted Liquefaction of Porous Carbons

Contact Info

Product

Resources

About