Role of Enzymes in Biofuel Production: Recent Developments and Challenges

Bangaru, Arvind; Sree, Kamasani Aarya; Kruthiventi, Chandana; Banala, Meenakshi; Shreya, Vadapalli; Vineetha, Y.; Shalini, A.; Mishra, Bishwambhar; Yadavalli, Rajasri; Chandrasekhar, K.; Reddy, C. Nagendranatha

doi:10.1007/978-981-16-8090-8_4

Cited by 8 publications

(2 citation statements)

References 105 publications

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“…Strong predictive capabilities may be more easily obtained with machine learning, [41] which can efficiently handle non-linearities, in particular physics-informed machine learning approaches [42] which can incorporate the physical insights revealed here. The beneficial impact of these insights on future predictions will be critical to guide the modification of biotechnologically useful enzymes [43][44][45][46] so they can remain functional in saline solutions thus reducing dependency on our fresh water resources, to obtain improved understanding of the connection between salt-dependent protein dynamics and protein-protein interactions in the course of evolution, [47][48][49][50] and to foster improved strategies for formulations of protein pharmaceutics and in structural biology.…”

Section: Discussionmentioning

confidence: 99%

Nonlinear Impact of Electrolyte Solutions on Protein Dynamics

Daronkola,

Söldner,

Singh

et al. 2024

ChemBioChem

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Halophilic organisms have adapted to multi‐molar salt concentrations, their cytoplasmic proteins functioning despite stronger attraction between hydrophobic groups. These proteins, of interest in biotechnology because of decreasing fresh‐water resources, have excess acidic amino acids. It has been suggested that conformational fluctuations ‐‐ critical for protein function ‐‐ decrease in the presence of a stronger hydrophobic effect, and that an acidic proteome would counteract this decrease. However, our understanding of the salt‐ and acidic amino acid dependency of enzymatic activity is limited. Here, using solution NMR relaxation and molecular dynamics simulations for in total 14 proteins, we show that salt concentration has a limited and moreover non‐monotonic impact on protein dynamics. The results speak against the conformational‐fluctuations model, instead indicating that maintaining protein dynamics to ensure protein function is not an evolutionary driving force behind the acidic proteome of halophilic proteins.

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Section: Discussionmentioning

confidence: 99%

Nonlinear Impact of Electrolyte Solutions on Protein Dynamics

Daronkola,

Söldner,

Singh

et al. 2024

ChemBioChem

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“…As they reduce the negative impacts on the environment, the use of lignocellulolytic enzymes in pre-treatment and hydrolysis stages has increased significantly [ 303 ]. Efficient enzyme cocktails for biofuel production include cellulases, hemicellulases, and ligninases, as well as pectinases and accessory proteins such as AA9 and swolenins [ 56 ].…”

Section: Application Of Lignocellulolytic Enzymesmentioning

confidence: 99%

Lignocellulolytic Biocatalysts: The Main Players Involved in Multiple Biotechnological Processes for Biomass Valorization

Benatti

Polizeli

2023

Microorganisms

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Human population growth, industrialization, and globalization have caused several pressures on the planet’s natural resources, culminating in the severe climate and environmental crisis which we are facing. Aiming to remedy and mitigate the impact of human activities on the environment, the use of lignocellulolytic enzymes for biofuel production, food, bioremediation, and other various industries, is presented as a more sustainable alternative. These enzymes are characterized as a group of enzymes capable of breaking down lignocellulosic biomass into its different monomer units, making it accessible for bioconversion into various products and applications in the most diverse industries. Among all the organisms that produce lignocellulolytic enzymes, microorganisms are seen as the primary sources for obtaining them. Therefore, this review proposes to discuss the fundamental aspects of the enzymes forming lignocellulolytic systems and the main microorganisms used to obtain them. In addition, different possible industrial applications for these enzymes will be discussed, as well as information about their production modes and considerations about recent advances and future perspectives in research in pursuit of expanding lignocellulolytic enzyme uses at an industrial scale.

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