Role of epigenetics in zebrafish development

Balasubramanian, Satheeswaran; Raghunath, Azhwar

doi:10.1016/j.gene.2019.144049

Cited by 28 publications

(16 citation statements)

References 232 publications

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“…A possible explanation could be that uhrf1 mRNA is maternally provided and in sufficient amounts to allow the development during the first 24 hpf [21,23], thus masking the possible uhrf1 gene regulation at the 5 prim 24 h stage. Additionally, several factors are actively contributing to the methylation process during zebrafish early development [37].…”

Section: Plos Onementioning

confidence: 99%

Hmx1 regulates urfh1 expression in the craniofacial region in zebrafish

2021

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H6 family homeobox 1 (HMX1) regulates multiple aspects of craniofacial development as it is widely expressed in the eye, peripheral ganglia and branchial arches. Mutations in HMX1 are linked to an ocular defect termed Oculo-auricular syndrome of Schorderet-Munier-Franceschetti (MIM #612109). We identified UHRF1 as a target of HMX1 during development. UHRF1 and its partner proteins actively regulate chromatin modifications and cellular proliferation. Luciferase assays and in situ hybridization analyses showed that HMX1 exerts a transcriptional inhibitory effect on UHRF1 and a modification of its expression pattern. Overexpression of hmx1 in hsp70-hmx1 zebrafish increased uhrf1 expression in the cranial region, while mutations in the hmx1 dimerization domains reduced uhrf1 expression. Moreover, the expression level of uhrf1 and its partner dnmt1 was increased in the eye field in response to hmx1 overexpression. These results indicate that hmx1 regulates uhrf1 expression and, potentially through regulating the expression of factors involved in DNA methylation, contribute to the development of the craniofacial region of zebrafish.

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Section: Plos Onementioning

confidence: 99%

Hmx1 regulates urfh1 expression in the craniofacial region in zebrafish

2021

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“…The zebrafish has already served as a model to investigate epigenetic changes in hearing loss [31], development [82], and cancer [78,83,84] and in propagating secondary complications observed in diabetes mellitus [85]. However, though the zebrafish model has been used abundantly in kidney research, so far only few studies have focused on the epigenetic contribution to renal diseases in this versatile in vivo model [47,57,58,86].…”

Section: Using the Zebrafish Model To Study Epigenetics In Renal Diseasesmentioning

confidence: 99%

The Zebrafish Model to Understand Epigenetics in Renal Diseases

Sopel

Müller-Deile

2021

IJMS

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Epigenetic modifications are able to alter gene expression and include DNA methylation, different histone variants, and post-transcriptional modifications (PTMs), such as acetylation or phosphorylation, and through short/long RNAs, respectively. In this review, we focus on current knowledge concerning epigenetic modifications in gene regulation. We describe different forms of epigenetic modifications and explain how epigenetic changes can be detected. The relevance of epigenetics in renal diseases is highlighted with multiple examples and the use of the zebrafish model to study glomerular diseases in general and epigenetics in renal diseases in particular is discussed. We end with an outlook on how to use epigenetic modifications as a therapeutic target for different diseases. Here, the zebrafish model can be employed as a high-throughput screening tool not only to discover epigenetic alterations contributing to disease, but also to test novel substances that change epigenetic signatures in vivo. Therefore, the zebrafish model harbors the opportunity to find novel pathogenic pathways allowing a pre-selection of potential targets and compounds to be tested for renal diseases.

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“…Zebrafish (D. rerio), a freshwater fish, is largely employed as a model organism in scientific research for its intrinsic properties, such as abundance of offspring, external fertilization and development, remarkable optical accessibility of embryo at any developmental stage from fertilization. In addition, D. rerio shares extensive genetic, physiological and molecular similarities with mammals, features that make it an excellent model for developmental studies (Balasubramanian et al 2019), drug discovery (MacRae & Peterson 2015), cancer biology (Lobert et al 2016), ecotoxicology (Bambino & Chu 2017), as well as endocrinology and GC/Gr research (McGonnell & Fowkes 2006, Schaaf et al 2009, Löhr & Hammerschmidt 2011.…”

Section: The Study Of Gc/gr Signaling In Danio Reriomentioning

confidence: 99%

Glucocorticoid receptor activities in the zebrafish model: a review

Dinarello

Licciardello

Fontana

et al. 2020

Journal of Endocrinology

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Glucocorticoids (GCs) are steroid hormones that contribute to the regulation of many physiological processes, such as inflammation, metabolism and stress response, mainly through binding to their cognate receptor, GR, which works as a ligand-activated transcription factor. Due to their pleiotropy and the common medical use of these steroids to treat patients affected by different pathologies, the investigation of their mechanisms of action is extremely important in biology and clinical research. The evolutionary conservation of GCs’ physiological functions, biosynthesis pathways as well as sequence and structure of their nuclear receptor, in the last 20 years has stimulated the use of zebrafish (a teleost of Ciprinidae family) as a reliable model organism to investigate the topic. In this review, we wanted to collect many of the most important discoveries that the scientific community has obtained using zebrafish to study GCs and their receptors. The paper begins by describing the experiments with transient knockdown of zebrafish gr to gain information mainly during development and continues with the discoveries provided by the generation of transgenic reporter lines. Finally, we discuss how the generation of mutant lines for either gr or the enzymes involved in GCs’ synthesis has significantly advanced our knowledge on GCs’ biology.

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Role of epigenetics in zebrafish development

Cited by 28 publications

References 232 publications

Hmx1 regulates urfh1 expression in the craniofacial region in zebrafish

Hmx1 regulates urfh1 expression in the craniofacial region in zebrafish

The Zebrafish Model to Understand Epigenetics in Renal Diseases

Glucocorticoid receptor activities in the zebrafish model: a review

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