Role of epithelial-mesenchymal transition markers E-cadherin, N-cadherin, β-catenin and ZEB2 in laryngeal squamous cell carcinoma

Zhu, Guanghao; Song, Panpan; Zhou, Han; Shen, Xiaohui; Wang, Junguo; Ma, Xiaofeng; Gu, Yajun; Liu, Ding‐Ding; Feng, An‐Ning; Qian, Xiaoyun; Gao, Xia

doi:10.3892/ol.2018.7751

Cited by 51 publications

(55 citation statements)

References 40 publications

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“…[15] Zhu et al in his systematic review reported that E-Cadherin could be a critical factor in predicting the prognosis of Laryngeal Squamous Cell Carcinoma (LSCC). [27] A downregulation in the E-Cadherin expression in patients with LSCC was reported by Nardi et al and signifies its prognostic importance in cervical metastasis. [28] A comparison of main characteristics of the studies included in literature review with the present study are highlighted in Table 3.…”

Section: Fig 2 Low Expression Of E-cadherinmentioning

confidence: 83%

E-Cadherin as a Marker for Nodal Metastasis in Head and Neck Squamous Cell Carcinoma

Butt

Ali

Avesi

et al. 2019

JAMMR

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Aim: Head and neck cancers, all over the world, contribute greatly to the number of deaths, despite the advancements in the therapeutic strategies. It is characterized by locoregional disease with a tendency for metastasis to the cervical lymph nodes. The pre-operative detection of lymph node metastasis is critical for the effective treatment of patients with head and neck squamous cell carcinoma. Therefore the objective of this study was to identify E-cadherin as a marker for prediction of lymph node metastasis in head and neck squamous cell carcinoma (HNSCC). Study Design: Cross-sectional study. Place and Duration of Study: Dow University of Health Sciences, Karachi. 1 Year duration. Methodology: Cross-sectional analysis of 54 subjects with HNSCC, who underwent neck dissections, was carried out. Expression of E-cadherin was evaluated using immunohistochemical analysis and traditional histological parameters, and correlation of E-Cadherin with histologically verified presence of regional metastases was determined. Data was subjected to descriptive statistics and chi-square using Spss v.16.0. Results: 54 patients included 33 males (61.1%) and 21 females (38.9%) aged from 18 to 73 (mean 44.8±12.7). A statistically significant relationship between the Downregulation of E-cadherin and histologically verified presence of nodal metastasis was established. (p value= 0.01). Conclusion: This study shows that low E-cadherin expression is useful for predicting lymph node metastases in cases of head and neck carcinoma.

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Section: Fig 2 Low Expression Of E-cadherinmentioning

confidence: 83%

E-Cadherin as a Marker for Nodal Metastasis in Head and Neck Squamous Cell Carcinoma

Butt

Ali

Avesi

et al. 2019

JAMMR

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“…EMT serves a key role in the metastasis of LUAD . Tumor progression is often associated with the loss of E‐cadherin and β‐catenin, and an increase in the expression levels of vimentin and N‐cadherin, which result in more motile and invasive characteristics . To investigate the effect of lenti‐shACTL8 on E‐cadherin, β‐catenin, vimentin and N‐cadherin, A549 and NCI‐H1975 cells were infected with lenti‐shACTL8 for three days and evaluated by western blotting.…”

Section: Resultsmentioning

confidence: 99%

“…14 Tumor progression is often associated with the loss of E-cadherin and β-catenin, and an increase in the expression levels of vimentin and N-cadherin, which result in more motile and invasive characteristics. 15 To investigate the effect of lenti-shACTL8 on E-cadherin, β-catenin, vimentin and Ncadherin, A549 and NCI-H1975 cells were infected with lenti-shACTL8 for three days and evaluated by western blotting. It was revealed that shACTL8 significantly increased the expression level of N-cadherin and β-catenin, and decreased that of vimentin in A549 cells.…”

Section: Shrna Inhibition Of Actl8 Reduces Emt In Human Luad Cellsmentioning

confidence: 99%

Actin‐like protein 8 promotes cell proliferation, colony‐formation, proangiogenesis, migration and invasion in lung adenocarcinoma cells

Wang

Zhang

et al. 2020

Thoracic Cancer

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Background Non‐small cell lung cancer (NSCLC) is the leading cause of cancer‐associated mortality worldwide of which lung adenocarcinoma (LUAD) is the most common. The identification of oncogenes and effective drug targets is the key to individualized LUAD treatment. Actin‐like protein 8 (ACTL8), a member of the cancer/testis antigen family, is associated with tumor growth and patient prognosis in various types of cancer. However, whether ACTL8 is involved in the development of LUAD remains unknown. The aim of the present study was to demonstrate the role of ACTL8 in human LUAD cells. Methods The expression of ACTL8 in LUAD tissues and cell lines was assessed using immunohistochemistry and western blotting. Additionally, plasmids expressing ACTL8‐specific short hairpin RNAs were used to generate lentiviruses which were subsequently used to infect A549 and NCI‐H1975 human LUAD cells. Cell proliferation, migration, invasion and apoptosis, as well as cell cycle progression and the expression of protein markers of epithelial to mesenchymal transition were investigated. A549 cell tumor growth in nude mice was also examined. Results The results showed that ACTL8 was highly expressed in A549 and NCI‐H1975 LUAD cell lines. Additionally, ACTL8‐knockdown inhibited proliferation, colony formation, cell cycle progression, migration and invasion, and increased apoptosis in both cell lines. Furthermore, in vivo experiments in nude mice revealed that ACTL8‐knockdown inhibited A549 cell tumor growth. Conclusion These results suggest that ACTL8 serves an oncogenic role in human LUAD cells, and that ACTL8 may represent a potential therapeutic target for LUAD. Key points Our results suggest that ACTL8 serves an oncogenic role in human LUAD cells, and that ACTL8 may represent a potential therapeutic target for LUAD.

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“…Authors have shown that adhesion proteins like E-cadherin are actively involved in mesenchymal to epithelial (MET) and epithelial to mesenchymal (EMT) transitions, which play an important role in cancer progression and tissue fibrosis 48 . The current immunohistochemistry results from kidney sections showed that E-cadherin expression appeared lower in the FB group and higher in the naïve and BBT-059 administered groups at six months.…”

Section: Discussionmentioning

confidence: 99%

Delayed effects of acute whole body lethal radiation exposure in mice pre-treated with BBT-059

Sharma

Holmes-Hampton

Kumar

et al. 2020

Sci Rep

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The threat of nuclear exposure is heightened and it is imperative to identify potential countermeasures for acute radiation syndrome. Currently no countermeasures have been approved for prophylactic administration. Effective countermeasures should function to increase survival in the short term as well as to increase the overall prognosis of an exposed individual long term. Here we describe the use of a promising radiation countermeasure, BBT-059, and the results of a long term mouse study (up to 12 months) in the male CD2F1 strain using 60 Co gamma irradiation (~0.6 Gy/min, 7.5-12.5 Gy). We report the dose reduction factor of 1.28 for BBT-059 (0.3 mg/kg) compared to control administered 24 h prior to irradiation. In the long term study animals showed accelerated recovery in peripheral blood cell counts, bone marrow colony forming units, sternal cellularity and megakaryocyte numbers in drug treated mice compared to formulation buffer. In addition, increased senescence was observed in the kidneys of animals administered control or drug and exposed to the highest doses of radiation. Decreased levels of E-cadherin, LaminB1 and increased levels of Cyc-D and p21 in spleen lysates were observed in animals administered control. Taken together the results indicate a high level of protection following BBT-059 administration in mice exposed to lethal and supralethal doses of total body gamma-radiation.

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Role of epithelial-mesenchymal transition markers E-cadherin, N-cadherin, β-catenin and ZEB2 in laryngeal squamous cell carcinoma

Cited by 51 publications

References 40 publications

E-Cadherin as a Marker for Nodal Metastasis in Head and Neck Squamous Cell Carcinoma

E-Cadherin as a Marker for Nodal Metastasis in Head and Neck Squamous Cell Carcinoma

Actin‐like protein 8 promotes cell proliferation, colony‐formation, proangiogenesis, migration and invasion in lung adenocarcinoma cells

Delayed effects of acute whole body lethal radiation exposure in mice pre-treated with BBT-059

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