Role of extracellular matrix components in biofilm formation and adaptation of Pseudomonas ogarae F113 to the rhizosphere environment

Blanco-Romero, Esther; Garrido-Sanz, Daniel; Durán, David; Rybtke, Morten; Tolker-Nielsen, Tim; Redondo-Nieto, Miguel; Rivilla, Rafael; Martín, Marta

doi:10.3389/fmicb.2024.1341728

Cited by 3 publications

(1 citation statement)

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“…Effective colonization of the rhizosphere environment by bacteria relies on key characteristics. Notably, motility and biofilm formation allow soil-dwelling bacteria to reach the roots, where they attach and colonize by developing microcolonies ( Turnbull et al, 2001 ; Naseem et al, 2018 ; Blanco-Romero et al, 2024 ). The genomes of the four novel species characterized in this study were functionally annotated ( Figure 3A , Supplementary Table 6 ), revealing that they harbor a complete set of genes encoding a single flagellar apparatus controlled by the flhDC master regulator operon ( Soutourina and Bertin, 2003 ).…”

Section: Resultsmentioning

confidence: 99%

Expanding the Pseudomonas diversity of the wheat rhizosphere: four novel species antagonizing fungal phytopathogens and with plant-beneficial properties

Poli,

Keel,

Garrido-Sanz

2024

Front. Microbiol.

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Plant-beneficial Pseudomonas bacteria hold the potential to be used as inoculants in agriculture to promote plant growth and health through various mechanisms. The discovery of new strains tailored to specific agricultural needs remains an open area of research. In this study, we report the isolation and characterization of four novel Pseudomonas species associated with the wheat rhizosphere. Comparative genomic analysis with all available Pseudomonas type strains revealed species-level differences, substantiated by both digital DNA-DNA hybridization and average nucleotide identity, underscoring their status as novel species. This was further validated by the phenotypic differences observed when compared to their closest relatives. Three of the novel species belong to the P. fluorescens species complex, with two representing a novel lineage in the Pseudomonas phylogeny. Functional genome annotation revealed the presence of specific features contributing to rhizosphere colonization, including flagella and components for biofilm formation. The novel species have the genetic potential to solubilize nutrients by acidifying the environment, releasing alkaline phosphatases and their metabolism of nitrogen species, indicating potential as biofertilizers. Additionally, the novel species possess traits that may facilitate direct promotion of plant growth through the modulation of the plant hormone balance, including the ACC deaminase enzyme and auxin metabolism. The presence of biosynthetic clusters for toxins such as hydrogen cyanide and non-ribosomal peptides suggests their ability to compete with other microorganisms, including plant pathogens. Direct inoculation of wheat roots significantly enhanced plant growth, with two strains doubling shoot biomass. Three of the strains effectively antagonized fungal phytopathogens (Thielaviopsis basicola, Fusarium oxysporum, and Botrytis cinerea), demonstrating their potential as biocontrol agents. Based on the observed genetic and phenotypic differences from closely related species, we propose the following names for the four novel species: Pseudomonas grandcourensis sp. nov., type strain DGS24T ( = DSM 117501T = CECT 31011T), Pseudomonas purpurea sp. nov., type strain DGS26T ( = DSM 117502T = CECT 31012T), Pseudomonas helvetica sp. nov., type strain DGS28T ( = DSM 117503T = CECT 31013T) and Pseudomonas aestiva sp. nov., type strain DGS32T ( = DSM 117504T = CECT 31014T).

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Section: Resultsmentioning

confidence: 99%

Expanding the Pseudomonas diversity of the wheat rhizosphere: four novel species antagonizing fungal phytopathogens and with plant-beneficial properties

Poli,

Keel,

Garrido-Sanz

2024

Front. Microbiol.

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Priority effects of heritable seed-borne bacteria drive early assembly of the wheat rhizosphere microbiome

Garrido-Sanz,

Keel

2024

Preprint

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Microbial communities play a critical role in supporting plant health and productivity, making the ability to obtain reproducible plant-associated microbiomes an essential asset for experimentally testing hypotheses related to microbiome manipulation and fundamental principles governing community dynamics. We used a sequential propagation strategy to generate a complex and reproducible wheat rhizosphere microbiome (RhizCom) that was shaped by host selection and periodic habitat resetting. Heritable seed-borne rhizosphere bacteria (SbRB) emerged as the dominant microbiome source after coalescing with the soil community, driven by priority effects and efficient niche exploitation during early habitat development. Functional analyses revealed that niche partitioning through the ability of SbRB to degrade specific saccharides and niche facilitation contributed to the assembly of the RhizCom. Our results advance our understanding of the principles governing microbial community dynamics in early plant development and provide strategies for future microbiome manipulation aimed at improving crop productivity and health.

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Carbon Source and Substrate Surface Affect Biofilm Formation by the Plant-Associated Bacterium Pseudomonas donghuensis P482

Rajewska,

Maciąg,

Narajczyk

et al. 2024

IJMS

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The ability of bacteria to colonize diverse environmental niches is often linked to their competence in biofilm formation. It depends on the individual characteristics of a strain, the nature of the colonized surface (abiotic or biotic), or the availability of certain nutrients. Pseudomonas donghuensis P482 efficiently colonizes the rhizosphere of various plant hosts, but a connection between plant tissue colonization and the biofilm formation ability of this strain has not yet been established. We demonstrate here that the potential of P482 to form biofilms on abiotic surfaces and the structural characteristics of the biofilm are influenced by the carbon source available to the bacterium, with glycerol promoting the process. Also, the type of substratum, polystyrene or glass, impacts the ability of P482 to attach to the surface. Moreover, P482 mutants in genes associated with motility or chemotaxis, the synthesis of polysaccharides, and encoding proteases or regulatory factors, which affect biofilm formation on glass, were fully capable of colonizing the root tissue of both tomato and maize hosts. Investigating the role of cellular factors in biofilm formation using these plant-associated bacteria shows that the ability of bacteria to form biofilm on abiotic surfaces does not necessarily mirror its ability to colonize plant tissues. Our research provides a broader perspective on the adaptation of these bacteria to various environments.

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Role of extracellular matrix components in biofilm formation and adaptation of Pseudomonas ogarae F113 to the rhizosphere environment

Cited by 3 publications

References 88 publications

Expanding the Pseudomonas diversity of the wheat rhizosphere: four novel species antagonizing fungal phytopathogens and with plant-beneficial properties

Expanding the Pseudomonas diversity of the wheat rhizosphere: four novel species antagonizing fungal phytopathogens and with plant-beneficial properties

Priority effects of heritable seed-borne bacteria drive early assembly of the wheat rhizosphere microbiome

Carbon Source and Substrate Surface Affect Biofilm Formation by the Plant-Associated Bacterium Pseudomonas donghuensis P482

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