Role of extracellular matrix molecules in the development of the sodium current in quail mesencephalic neural crest cells

Distasi, Carla; Lovisolo, Davide; Alonzo, F.; Tolosano, Emanuela; Fasolo, Aldo

doi:10.1007/bf02118419

Cited by 2 publications

(1 citation statement)

References 23 publications

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“…Spitzer et al, 1994); our findings demonstrate the existence in postmitotic neural crest neurons of a different pathway. All Fura-2 and single-channel measurements and 79% of whole-cell experiments were performed on cells cultured for 1 or 2 days; at this stage of development, voltage-activated calcium channels are not yet expressed (Bader et al, 1983;Distasi et al, 1992). so a direct effect of acute stimulation with bFGF on this class of channels can be ruled out.…”

Section: Discussionmentioning

confidence: 99%

Basic Fibroblast Growth Factor Opens Calcium‐Permeable Channels in Quail Mesencephalic Neural Crest Neurons

Distasi¹,

Munaron²,

Laezza³

et al. 1995

Eur J of Neuroscience

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In order to investigate the action of basic fibroblast growth factor (bFGF) in the nervous system, we have studied the ionic signals elicited by this peptide in cultured quail mesencephalic neural crest neurons using patch-clamp and cytofluorimetric techniques. In this preparation stimulation with bFGF induced, with a delay of some tens of seconds, an inward cationic current. Single-channel experiments provided evidence for the activation of a calcium-permeable channel. In single-cell cytofluorimetric measurements, a sustained rise in [Ca2+]i was observed, which was dependent on the presence of external calcium. These events may play a role in the developmental effects of bFGF.

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Section: Discussionmentioning

confidence: 99%

Basic Fibroblast Growth Factor Opens Calcium‐Permeable Channels in Quail Mesencephalic Neural Crest Neurons

Distasi¹,

Munaron²,

Laezza³

et al. 1995

Eur J of Neuroscience

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Electrophysiological characterization of voltage-gated Na+ current expressed in the highly metastatic Mat-LyLu cell line of rat prostate cancer

Grimes

Djamgoz

1998

J. Cell. Physiol.

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Voltage-gated Na+ channels, classically associated with impulse conduction in excitable tissues, are also found in a variety of epithelial cell types where their possible functions are not known so well. We have previously reported expression of a voltage-gated Na+ channel specifically in the highly metastatic Mat-LyLu rat prostate cancer cell line; blockage of the current with tetrodotoxin (TTX) significantly reduced the invasiveness of the cells in vitro, suggesting that the channel may have a functional role in metastasis. The aim of the present study was to characterize this current using the whole-cell patch clamp recording technique, and compare it to Na+ currents found in various other tissues. The inward current of the Mat-LyLu cells was abolished completely, but reversibly, in Na+-free solution, confirming that Na+ was indeed the permeant ion. Activation occurred at -40 mV and currents reached a maximal amplitude at around 6 mV. Boltzmann fits to current activation and steady-state inactivation revealed that the currents were half activated at about -15 mV and half inactivated at -80 mV. Both current inactivation and recovery from inactivation followed a double-exponential time course with fast and slow components. The Na+ currents were highly sensitive to block by TTX (IC50 approximately 18 nM), whilst 1 microM mu-conotoxin GIIIA mostly had no effect. 100 microM Cd2+ also had no effect on the current, whilst 2.5 mM Cd2+, Mn2+, and Co2+ each caused a depolarizing shift in activation and a reduction in peak conductance of around 20%. In conclusion, the Na+ channel expressed in the highly metastatic Mat-LyLu cell line appeared to have electrophysiological and pharmacological properties of TTX-sensitive channels. Further work is needed, however, to elucidate the exact nature of the channel protein and the mechanism(s) of its involvement in cellular invasiveness.

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Role of extracellular matrix molecules in the development of the sodium current in quail mesencephalic neural crest cells

Cited by 2 publications

References 23 publications

Basic Fibroblast Growth Factor Opens Calcium‐Permeable Channels in Quail Mesencephalic Neural Crest Neurons

Basic Fibroblast Growth Factor Opens Calcium‐Permeable Channels in Quail Mesencephalic Neural Crest Neurons

Electrophysiological characterization of voltage-gated Na+ current expressed in the highly metastatic Mat-LyLu cell line of rat prostate cancer

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