Role of extracellular polymeric substance in adsorption of quinolone antibiotics by microbial cells in excess sludge

Cao, Da-Qi; Yang, Wenyu; Wang, Zhen; Hao, Xiaodi

doi:10.1016/j.cej.2019.03.230

Cited by 82 publications

(28 citation statements)

References 57 publications

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“…In general terms, at the beginning of experimentation, the effluent concentration was very low, removing 30 to 70% of the influent concentration. This fact could be explained by the adsorption in the external layers of the AGS caused by the superficial charges of EPS [ 33 , 34 ]. However, over operational time, the concentration measured in the effluent showed lower values of biodegradation, biotransformation, and/or bioadsorption by the granular sludge.…”

Section: Resultsmentioning

confidence: 99%

Total and Metabolically Active Microbial Community of Aerobic Granular Sludge Systems Operated in Sequential Batch Reactors: Effect of Pharmaceutical Compounds

Muñoz-Palazon

Rosa-Masegosa

Hurtado-Martinez

et al. 2021

Toxics

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Two aerobic granular sludge (AGS) sequential batch reactors were operated at a mild (15 °C) temperature for 180 days. One of those bioreactors was exposed to a mixture of diclofenac, naproxen, trimethoprim, and carbamazepine. The AGS system, operating under pressure from emerging contaminants, showed a decrease in COD, BOD5, and TN removal capacity, mainly observed during the first 100 days, in comparison with the removal ratios detected in the control bioreactor. After an acclimatisation period, the removal reached high-quality effluent for COD and TN, close to 95% and 90%, respectively. In the steady-state period, trimethoprim and diclofenac were successfully removed with values around 50%, while carbamazepine and naproxen were more recalcitrant. The dominant bacterial OTUs were affected by the presence of a mixture of pharmaceutical compounds, under which the dominant phylotypes changed to OTUs classified among the Pseudomonas, Gemmobacter, and Comamonadaceae. The RT-qPCR and qPCR results showed the deep effects of pharmaceutical compounds on the number of copies of target genes. Statistical analyses allowed for linking the total and active microbial communities with the physico-chemical performance, describing the effects of pharmaceutical compounds in pollution degradation, as well as the successful adaptation of the system to treat wastewater in the presence of toxic compounds.

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Section: Resultsmentioning

confidence: 99%

Total and Metabolically Active Microbial Community of Aerobic Granular Sludge Systems Operated in Sequential Batch Reactors: Effect of Pharmaceutical Compounds

Muñoz-Palazon

Rosa-Masegosa

Hurtado-Martinez

et al. 2021

Toxics

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“…For example, a wide range of microbes in activated sludge can secrete cysteinerich proteins, which can bind strongly with metals such Cu 2+ , Au + , and Ag + via the sulfhydryl groups. 38 In addition, many types of organic micropollutants (e.g., antibiotics, dyes) can also be efficiently adsorbed by the proteinaceous components in EPS via the amine and carboxyl groups 8,39,40 or some EPS-bounded metal ions 41 (Figure 2). Notably, the species and structures of these functional groups and hence their pollutant-binding affinity would vary significantly with the associated EPS molecules and the external environment.…”

Section: Molecular Interactions Between Sludgementioning

confidence: 99%

“…For example, a wide range of microbes in activated sludge can secrete cysteine-rich proteins, which can bind strongly with metals such Cu 2+ , Au + , and Ag + via the sulfhydryl groups . In addition, many types of organic micropollutants (e.g., antibiotics, dyes) can also be efficiently adsorbed by the proteinaceous components in EPS via the amine and carboxyl groups ,, or some EPS-bounded metal ions (Figure ).…”

Section: Molecular Interactions Between Sludge Eps and Pollutantsmentioning

confidence: 99%

Molecular Insights into Extracellular Polymeric Substances in Activated Sludge

2020

Environ. Sci. Technol.

301

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Extracellular polymeric substances (EPS) are known to crucially affect the properties and performance of activated sludge, but the detailed influential mechanisms and the pertinence to specific compositional, structural properties of EPS are still elusive. Such knowledge gaps have severely limited our ability in optimizing biological wastewater treatment processes, for which long-term robust and efficient sludge performance remains one of the main bottlenecks. Here, we overview the new knowledge on the molecular structure of sludge EPS gained over the past few years and discuss the future challenges and opportunities for further advancing EPS study and engineering. The structural and functional features of several macromolecules in sludge EPS and their important structural roles in granular sludge are analyzed in detail. The EPS−pollutant interactions and environment-dependent regulation machinery on EPS production are deciphered. Lastly, the remaining knowledge gaps are identified, and the future research needs that may lead to molecular-level understanding and precise engineering of sludge EPS are highlighted.

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“…The pH value is an important parameter in the adsorption process. The pH not only affects the functional groups of MFX but also affects the surface charge of carbamazepine [ 24 , 25 ]. The removal rate of carbamazepine by MFX was tested at pH = 5–10, and the results are shown in Figure 1(a) .…”

Section: Resultsmentioning

confidence: 99%

Flocculation Efficiency and Mechanism of Carbamazepine by Microbial Flocculant Extracted from Klebsiella pneumoniae J1

Xing

Song

Chen

et al. 2020

Archaea

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The microbial flocculant (MFX) extracted from Klebsiella pneumoniae J1 was used to remove carbamazepine in prepared wastewater and domestic sewage. The influence factors and flocculation mechanism were studied. The optimal carbamazepine removal conditions for MFX were pH of 7-8, 7 mL of flocculant, 0.1 mL of coagulant, and 35°C, and the removal rate reached 81.75%. MFX was efficient in the removal of carbamazepine in both domestic sewage (75.03%) and secondary sedimentation tank effluent (69.76%). The pseudo-first-order kinetic equation fitted the adsorption process better than the pseudo-second-order kinetic equation, which suggested that the adsorption was not pure chemical adsorption. The analysis of floc size suggested that the repulsive force between carbamazepine and MFX was weakened under alkalescent conditions, which can help the growth and coherence of flocs and increase the carbamazepine removal efficiency. Enough dosage of MFX can generate larger flocs, but excessive dosage of MFX will decrease the carbamazepine removal rate because of increase in electrostatic repulsion. The analysis of 3D-EEM and FTIR suggested that hydroxyl, amino, and carboxyl in MFX played an important role in the removal of carbamazepine. As an eco-friendly and highly efficient microbial flocculant, MFX has potential for practical applications in carbamazepine removal.

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Role of extracellular polymeric substance in adsorption of quinolone antibiotics by microbial cells in excess sludge

Cited by 82 publications

References 57 publications

Total and Metabolically Active Microbial Community of Aerobic Granular Sludge Systems Operated in Sequential Batch Reactors: Effect of Pharmaceutical Compounds

Total and Metabolically Active Microbial Community of Aerobic Granular Sludge Systems Operated in Sequential Batch Reactors: Effect of Pharmaceutical Compounds

Molecular Insights into Extracellular Polymeric Substances in Activated Sludge

Flocculation Efficiency and Mechanism of Carbamazepine by Microbial Flocculant Extracted from Klebsiella pneumoniae J1

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