Background
The biofloc system (BFS) provides a sustainable aquaculture system through its efficient in situ water quality maintenance by the microbial biomass, besides continuous availability of these protein-rich microbes as feed to enhance growth and immunity of the reared organism. This study explores the gill architecture, growth performance, digestive enzyme activity, intestinal microbial composition, and histology of three freshwater fish species,
Puntius gonionotus
,
Pangasianodon hypophthalmus
, and
Heteropneustus fossilis
reared in biofloc based polyculture system.
Results
The three species in T2 showed significantly higher WG and SGR, followed by T1 and T3. The wet mount of gill architecture showed smaller inter-filament gaps in gill arches of silver barb followed by stinging catfish and stripped catfish, but showed no correlation with the weight gain. However, silver barb being an omnivore and filter-feeder, accumulated a more diverse microbial community, both in T1 and BFS (T2 and T3), while the bottom feeder
H. fossilis
exhibited unique gut bacterial adaptability. The presence of floc in T2 and T3 enhanced bacterial abundance in water and fish gut, but their microbial diversities significantly reduced compared to T1 receiving only feed. Next-generation sequencing revealed that the
Pseudomonas
dominated in gut of
P. gonionotus
and
P. hypophthalmus
in T1,
Enterobacterales
and
Fusobacterium
prevailed in those of T2 and T3, respectively. In contrast, gut of
H. fossilis
had the highest proportion of
Clostridium
in T1, while
Rhizobiaceae
dominated in T3. Similarly in floc samples,
Enterococcus
dominated in T1 while
Micrococcales
and
Rhizobiaceae
dominated in T2 and T3, respectively. A positive correlation of enterobacteria, with the digestive enzyme activities and growth patterns was observed in all treatments.
Conclusion
The present study revealed feeding behaviour to play crucial role in distinguishing the gut microbial composition patterns in fishes reared in Biofloc System. Further it revealed the requirement of supplementary feed along with floc in these three species for higher growth in the biofloc system.
Supplementary Information
The online version contains supplementary material available at 10.1186/s12866-024-03473-4.