Role of ganglioside biosynthesis genetic polymorphism in cervical cancer development

Danolić, Damir; Heffer, Marija; Wagner, Jasenka; Škrlec, Ivana; Alvir, Ilija; Mamić, Ivica; Šušnjar, Lucija; Banović, Marija; Puljiz, Mario

doi:10.1080/01443615.2019.1692801

Cited by 13 publications

(12 citation statements)

References 30 publications

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“…Abnormal expression of STsis involved in the regulation of the malignant progression of cancer (26). Accumulating evidence has demonstrated that ST8SIA1 can influence the growth and metastasis of a number of cancer types, including triple-negative breast cancer (18), colorectal cancer (27) and cervical cancer (28). In the present study, 13 differentially expressed glycosyltransferase genes were screened using TCGA; however, only 1 ST, ST8SIA1, was identified to exhibit low expression levels in BLCA.…”

Section: Discussionmentioning

confidence: 70%

ST8SIA1 inhibits the proliferation, migration and invasion of bladder cancer cells by blocking the JAK/STAT signaling pathway

Wang²,

Sun³

et al. 2021

Oncol Lett

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Bladder cancer (BLCA) is the most common malignant tumor of the urinary system, with distant metastasis of the tumor being the main cause of death. The identification of an effective biomarker may provide a novel direction for BLCA diagnosis and treatment. The aim of the present study was to screen the BLCA-related genes involved in sialyl transferase (ST) dysregulation and to investigate the functional mechanisms of α-2,8-ST1 (ST8SIA1) in BLCA cells. Data from The Cancer Genome Atlas and Gene Expression Profiling Interactive Analysis databases suggested that the mRNA expression levels of ST8SIA1 were decreased in BLCA tissues compared with normal tissues, which was also demonstrated using immunohistochemistry and western blot analysis. The expression levels of ST8SIA1 were negatively associated with the pathological grade and invasiveness of BLCA. Western blot analysis revealed that the expression levels of ST8SIA1 were lower in BLCA cell lines than in a normal urothelial cell line. CCK-8, flow cytometry, wound healing, colony formation and Transwell assays indicated that ST8SIA1 overexpression attenuated the proliferation, migration and invasion of T24 and 5637 BLCA cells. Further experiments revealed that ST8SIA1 could inhibit the phosphorylation of Janus kinase (JAK)2 and STAT3, as well as decrease the expression levels of JAK/STAT pathway-targeting signal molecules, including MMP2, proliferating cell nuclear antigen, cyclin D1 and Bcl2 in two BLCA cell lines. In conclusion, to the best of our knowledge, the present study was the first to indicate that the antitumor effect of ST8SIA1 in BLCA cells was mediated by the JAK/STAT signaling pathway, and the results provided a novel target for the diagnosis and treatment of BLCA.

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Section: Discussionmentioning

confidence: 70%

ST8SIA1 inhibits the proliferation, migration and invasion of bladder cancer cells by blocking the JAK/STAT signaling pathway

Wang²,

Sun³

et al. 2021

Oncol Lett

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“…miR-23a may affect the formation of Nglycochain branches on the cell surface through glucose transferase MGAT3, thereby increasing the metastasis potential of liver cancer, which provides a new idea for the mechanism of action of MGAT3 in tumor metastasis [23,24]. B4GalT5 participates in the synthesis of both N-linked oligosaccharides and various glycolipids, it involves in extraembryonic development and tumorigenesis in many kinds of tumors, such as astrocytoma, glioma, breast cancer and cervical cancer [25,9,26,27]. In fact, B4GalT5 has been reported to be one of the target genes connected to adverse clinical outcomes in patients with HCC [28].…”

Section: Discussionmentioning

confidence: 99%

B4GALT5 High Expression Associated With Poor Prognosis of Hepatocellular Carcinoma

Han

et al. 2021

Preprint

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Background: B4GALT5 is postulated to be an important protein in sugar metabolism that catalyzes the synthesis of lactosylceramide (LacCer). However, its role in hepatocellular carcinoma (HCC) remains unknown.Method: We characterized the expression of B4GALT5 in HCC tissue compared to normal tissue, and explored its function of B4GALT5 in HCC by enrichment analysis based on its co-expressed gene set. Next, we checked whether B4GALT5 expression is correlated to immune infiltration level and clinical prognosis in hepatocellular carcinoma. Finally, we verified the expression of B4GALT5 using clinical samples evaluated by RT-PCR, and conducted in vitro experiments with B4GALT5-knockdown HCC cells to investigate the function of B4GALT5 in the HCC cell proliferation, migration and invasion.Results: We found B4GALT5 mRNA and protein expression levels were significantly high in HCC tissue compared to normal tissue. The enrichment analysis of the gene sets that co-expressed with B4GALT5 showed specificity in HCC-related pathways and functions. Also, the expression pattern of B4GALT5 was significantly related to the immune infiltration level, especially CD4+ T cell and macrophage cells. B4GALT5 higher mRNA expression was associated with poor overall survival (OS) in HCC patients. Furthermore, In vitro experiments showed that depletion of B4GALT5 significantly inhibited HCC cell proliferation, migration and invasion. This study revealed the function and its mediated pathways of B4GALT5 in HCC, indicating that B4GALT5 may serve as a prognostic biomarker of HCC.

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“…Zhang et al (2009) found that Zinc finger transcription factor INSM1 interrupted cyclin D1 and CDK4 binding and induced cell cycle arrest. Besides, adjacent single-nucleotide polymorphisms (SNPs) to gene B4GALT1 could be associated with cervical cancer development (Danolic et al, 2020). Research revealed both SLC30A3 and GABRB2 had diagnostic and prognostic values for colon adenocarcinoma (Yan et al, 2020;Yin et al, 2020).…”

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confidence: 99%

Identification of a MicroRNA Signature Associated With Lymph Node Metastasis in Endometrial Endometrioid Cancer

Song

et al. 2021

Front. Genet.

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BackgroundLymph node metastasis (LNM) is an important prognostic factor in endometrial cancer. Anomalous microRNAs (miRNAs) are associated with cell functions and are becoming a powerful tool to characterize malignant transformation and metastasis. The aim of this study was to construct a miRNA signature to predict LNM in endometrial endometrioid carcinoma (EEC).MethodCandidate target miRNAs related to LNM in EEC were screened by three methods including differentially expressed miRNAs (DEmiRs), weighted gene co-expression network analysis (WGCNA), and decision tree algorithms. Samples were randomly divided into the training and validation cohorts. A miRNA signature was built using a logistic regression model and was evaluated by the area under the curve (AUC) of receiver operating characteristic curve (ROC) and decision curve analysis (DCA). We also conducted pathway enrichment analysis and miRNA–gene regulatory network to look for potential genes and pathways engaged in LNM progression. Survival analysis was performed, and the miRNAs were tested whether they expressed differently in another independent GEO database.ResultThirty-one candidate miRNAs were screened and a final 15-miRNA signature was constructed by logistic regression. The model showed good calibration in the training and validation cohorts, with AUC of 0.824 (95% CI, 0.739–0.912) and 0.821 (95% CI, 0.691–0.925), respectively. The DCA demonstrated the miRNA signature was clinically useful. Hub miRNAs in signature seemed to contribute to EEC progression via mitotic cell cycle, cellular protein modification process, and molecular function. MiR-34c was statistically significant in survival that a higher expression of miR-34c indicated a higher survival time. MiR-34c-3p, miR-34c-5p, and miR-34b-5p were expressed differentially in GSE75968.ConclusionThe miRNA signature could work as a noninvasive method to detect LNM in EEC with a high prediction accuracy. In addition, miR-34c cluster may be a key biomarker referring LNM in endometrial cancer.

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Role of ganglioside biosynthesis genetic polymorphism in cervical cancer development

Cited by 13 publications

References 30 publications

ST8SIA1 inhibits the proliferation, migration and invasion of bladder cancer cells by blocking the JAK/STAT signaling pathway

ST8SIA1 inhibits the proliferation, migration and invasion of bladder cancer cells by blocking the JAK/STAT signaling pathway

B4GALT5 High Expression Associated With Poor Prognosis of Hepatocellular Carcinoma

Identification of a MicroRNA Signature Associated With Lymph Node Metastasis in Endometrial Endometrioid Cancer

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