Role of geometrical cues in bone marrow-derived mesenchymal stem cell survival, growth and osteogenic differentiation

Abstract

A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription. AbstractMesenchymal stem cells (MSCs) play a vital role in bone formation process by differentiating into osteoblasts, in a tissue that offers not a flat but a discontinuous threedimensional (3D) topography in vivo. In order to understand how geometry may be affecting MSCs, this study explored the influence of 3D geometry on MSC-fate by comparing cell growth, viability and osteogenic potential using monolayer (twodimensional, 2D) with microsphere (3D) culture systems normalised to surface area. The results suggested lower cell viability and reduced cell growth in 3D. Alkaline phosphatase activity was higher in 3D; however, both collagen and mineral deposition appeared significantly lower in 3D, even after osteogenic supplementation. Also, there were signs of patchy mineralisation in 3D with or without osteogenic supplementation as early as day 7. These results suggest that the convex surfaces on microspheres and interparticulate porosity may have led to variable cell morphology and fate within the 3D culture. This study provides deeper insights into geometrical regulation of MSC responses applicable for bone tissue engineering.

3D Culture of Bone Marrow-Derived Mesenchymal Stem Cells (BMSCs) Could Improve Bone Regeneration in 3D-Printed Porous Ti6Al4V Scaffolds

“…Moreover, 3D culture in Matrigel strongly mimics the physiological condition in vivo . Many studies have reported that 3D culture provides an optimal environment for cellular adhesion and communication [ 23 , 26 ]. The 3D constructs represent a completely different environment, due to different signaling pathway activity compared to 2D culture.…”

Rapid conversion of highly porous borate glass microspheres into hydroxyapatite

“…33 Low-adherent 48-well plates were prepared using a coating with 200 μl of 1% w/v solution of poly(2-hydroxyethyl methacrylate) (poly-HEMA) (Sigma-Aldrich, UK) and 95% ethanol. 36 Cells were cultured in standard medium (low glucose DMEM supplemented with 10% foetal calf serum, 1% penicillin and streptomycin, 1% L-glutamine, 1% of non-essential amino acid) at 37°C and 5% CO 2 for 12 days.…”

Three-Dimensional Printed Titanium Scaffolds Enhance Osteogenic Differentiation and New Bone Formation by Cultured Adipose Tissue-Derived Stem Cells Through the IGF-1R/AKT/Mammalian Target of Rapamycin Complex 1 (mTORC1) Pathway

“…A three-dimensional (3D) culture environment can promote the osteogenic differentiation of adipose tissue-derived stem cells (ADSCs) induced by dexamethasone in vitro [11]. However, traditional two-dimensional (2D) culture involves seeding cells into plastic plates, which greatly reduces their differentiation potential [12]. In the present study, Cellmatrix hydrogel was used to load ADSCs into the 3D culture medium.…”