Role of glutamate and nitric oxide in onset of motor neuron degeneration in neurolathyrism

Khandare, Arjun L.; Ankulu, M; Aparna, N

doi:10.1016/j.neuro.2012.11.004

Cited by 12 publications

(9 citation statements)

References 65 publications

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“…For instance, administration of arginine or nitric oxide synthase inhibitors at cerebral infarction may cause opposite physiological outcomes [1]. The glutamate-induced excitotoxicity could be either aggravated [2] or alleviated [1] by nitric oxide signaling. Obviously, one should take into account that generation of nitric oxide involves an intercept between metabolism of lysine and arginine [3], which, in their turn, are tightly linked to other amino acids through multiple intercepts 2 of 16 in the amino acid metabolism.…”

Section: Introductionmentioning

confidence: 99%

Hypoxic Adaptation of Mitochondrial Metabolism in Rat Cerebellum Decreases in Pregnancy

Граф

Trofimova

Ksenofontov

et al. 2020

Cells

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Function of brain amino acids as neurotransmitters or their precursors implies changes in the amino acid levels and/or metabolism in response to physiological and environmental challenges. Modelling such challenges by pregnancy and/or hypoxia, we characterize the amino acid pool in the rat cerebellum, quantifying the levels and correlations of 15 amino acids and activity of 2-oxoglutarate dehydrogenase complex (OGDHC). The parameters are systemic indicators of metabolism because OGDHC limits the flux through mitochondrial TCA cycle, where amino acids are degraded and their precursors synthesized. Compared to non-pregnant state, pregnancy increases the cerebellar content of glutamate and tryptophan, decreasing interdependence between the quantified components of amino acid metabolism. In response to hypoxia, the dependence of cerebellar amino acid pool on OGDHC and the average levels of arginine, glutamate, lysine, methionine, serine, phenylalanine, and tryptophan increase in non-pregnant rats only. This is accompanied by a higher hypoxic resistance of the non-pregnant vs. pregnant rats, pointing to adaptive significance of the hypoxia-induced changes in the cerebellar amino acid metabolism. These adaptive mechanisms are not effective in the pregnancy-changed metabolic network. Thus, the cerebellar amino acid levels and OGDHC activity provide sensitive markers of the physiology-dependent organization of metabolic network and its stress adaptations.

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Section: Introductionmentioning

confidence: 99%

Hypoxic Adaptation of Mitochondrial Metabolism in Rat Cerebellum Decreases in Pregnancy

Граф

Trofimova

Ksenofontov

et al. 2020

Cells

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“…One of the major drawbacks of grass pea is the presence of the neuroexcitatory, non-protein amino acid, b-N-oxalyl-L-a, b-diaminopropionic acid (b-ODAP) considered the causative agent of lathyrism in animals and humans (Rao et al 1964;Van Moorhem et al 2011), a disorder of the sympathetic nerves in muscles that induces atrophy and irreversible paralysis in the lower limbs of humans and hind limbs of animals (Van Moorhem et al 2011;Khandare et al 2013). Previous studies have shown that the concentration of b-ODAP increased in grass pea seeds exposed to drought (Hussain et al 1997;Yan et al 2006), and the increase in the b-ODAP concentration in various tissues of grass pea has been considered an important physiological response to drought stress (Xing et al 2001;Zhou et al 2001;Haque et al 2011).…”

Section: Introductionmentioning

confidence: 99%

24-epibrassinolide increases growth, grain yield and β-ODAP production in seeds of well-watered and moderately water-stressed grass pea

et al. 2015

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24-epibrassinolide (EBL) is a growth regulator that promotes crop growth and yield, especially under water stress, but its effect on grass pea (Lathyrus sativus L.), a legume crop widely recognized as adapted to rainfed environments, is unknown. The aim of the present study was to investigate the effect of exogenously-applied EBL on growth, yield and accumulation of b-N-oxalyl-L-a, bdiaminopropionic acid (b-ODAP)-a neurotoxin that can induce lathyrism in animals and humans-in grass pea under well-watered (WW) and water-stressed conditions. The first experiment conducted in a growth chamber showed that EBL application increased plant height and leaf area of well-watered [WW, 85 % field capacity (FC)] grass pea seedlings and in those in which the soil dried from 85 to 30 % FC. In two pot experiments conducted under a rainout shelter, three water regimes-soil water contents maintained at (1) 85 or 80 % FC, (2) 50 % FC, and (3) 35 % FC-were imposed. In the first rainout shelter experiment, root/soil drenching with EBL significantly increased aboveground dry weight (DW) and water use efficiency (WUE) compared with plants without EBL at 85 and 50 % FC, but did not significantly affect grain yield. In a second rainout shelter experiment, water treatments were imposed from the vegetative phase to maturity and EBL treatment significantly increased aboveground DW, grain yield, b-ODAP concentration and amount, and WUE in the 80 and 50 % FC water regimes. It is concluded that exogenously-applied EBL can stimulate growth and WUE in grass pea when WW and moderately water stressed, and also stimulates b-ODAP production such that the concentration in the grain is similar or even higher.

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“…It has been reported that among several isoforms of these enzymes—iNOS (inducible form) and nNOS (neuronal form); and HO-1 (inducible form) and HO-2 (constitutive form)—are associated with various types of neuronal cells [ 2 , 5 , 6 , 7 ]. Glutamate is one of major excitatory neurotransmitters in the nervous system [ 8 , 9 ]. Synaptically released glutamate activates the post-synaptic N -methyl- D -aspartate (NMDA) receptors, mediating signal transmission in the neural system.…”

Section: Introductionmentioning

confidence: 99%

“…One of many effects of glutamate-evoked neural responses is the upregulation of NOS for releasing NO [ 10 , 11 , 12 ]. Excessive amounts of glutamate may induce neurotoxicity, which causes cell death [ 8 , 9 , 13 ], therefore NO and CO may play roles in glutamate-induced neurotoxicity and/or neuroprotection [ 1 , 8 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Amperometric Microsensors Monitoring Glutamate-Evoked In Situ Responses of Nitric Oxide and Carbon Monoxide from Live Human Neuroblastoma Cells

Heo

Woo

et al. 2017

Sensors

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In the brain, nitric oxide (NO) and carbon monoxide (CO) are important signaling gases which have multifaceted roles, such as neurotransmitters, neuromodulators, and vasodilators. Even though it is difficult to measure NO and CO in a living system due to their high diffusibility and extremely low release levels, electrochemical sensors are promising tools to measure in vivo and in vitro NO and CO gases. In this paper, using amperometric dual and septuple NO/CO microsensors, real-time NO and CO changes evoked by glutamate were monitored simultaneously for human neuroblastoma (SH-SY5Y) cells. In cultures, the cells were differentiated and matured into functional neurons by retinoic acid and brain-derived neurotrophic factor. When glutamate was administrated to the cells, both NO and CO increases and subsequent decreases returning to the basal levels were observed with a dual NO/CO microsensor. In order to facilitate sensor’s measurement, a flower-type septuple NO/CO microsensor was newly developed and confirmed in terms of the sensitivity and selectivity. The septuple microsensor was employed for the measurements of NO and CO changes as a function of distances from the position of glutamate injection. Our sensor measurements revealed that only functionally differentiated cells responded to glutamate and released NO and CO.

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Role of glutamate and nitric oxide in onset of motor neuron degeneration in neurolathyrism

Cited by 12 publications

References 65 publications

Hypoxic Adaptation of Mitochondrial Metabolism in Rat Cerebellum Decreases in Pregnancy

Hypoxic Adaptation of Mitochondrial Metabolism in Rat Cerebellum Decreases in Pregnancy

24-epibrassinolide increases growth, grain yield and β-ODAP production in seeds of well-watered and moderately water-stressed grass pea

Amperometric Microsensors Monitoring Glutamate-Evoked In Situ Responses of Nitric Oxide and Carbon Monoxide from Live Human Neuroblastoma Cells

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