Role of glutathione in adaptation and signalling during chilling and cold acclimation in plants

Kocsy, Gábor; Galiba, Gábor; Brunold, Christian

doi:10.1034/j.1399-3054.2001.1130202.x

Cited by 236 publications

(136 citation statements)

References 57 publications

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“…In AGB002 the increase of O 2 − radicals was 102% compared to control plants on day 7 of the experiment whereas, in AGB025, O 2 − increased by 140% compared to control plants. It was interesting to observe the correlation between O 2 • − and MDA concentration in AGB025 which was in agreement with the previous studies of Kocsy et al (1997Kocsy et al ( , 2001,). The reduction in peroxidation and cell viability towards the end of the cold exposure observed in AGB002 may be due to the better antioxidant enzyme machinery.…”

Section: Cell Damage and Oxidative Stresssupporting

confidence: 80%

“…2c and d). The increased activity and expression of antioxidant enzymes and levels of antioxidant molecules in W. somnifera and other plant species under various stresses has also been reported earlier (Kocsy et al, 1996(Kocsy et al, , 2001Jaleel et al, 2008Jaleel et al, , 2009Kaur et al, 2012;Sabir et al, 2012;Mishra et al, 2014). The combined action of CAT and SOD converts the O 2− and H 2 O 2 to water and O 2 , thus, prevent the cellular damage under unfavorable condition (Arora et al, 2014).…”

Section: Antioxidant Enzymesmentioning

confidence: 89%

“…The combined action of CAT and SOD converts the O 2− and H 2 O 2 to water and O 2 , thus, prevent the cellular damage under unfavorable condition (Arora et al, 2014). GR plays an important role in maintaining the cellular redox status, especially during low temperature induced photo-inhibition (Noctor and Foyer, 1998;Kocsy et al, 2001). Greater increase in GR would certainly help in maintaining a higher GSH/GSSG ratio, which is essential in ensuring the correct folding and appropriate activity of the proteins and redox signaling of various defense genes (Polidoros and Scandalios, 1999).…”

Section: Antioxidant Enzymesmentioning

confidence: 99%

“…In order to understand the correlation between increase in lipid peroxidation and ROS accumulation under cold stress (Kocsy et al, 1997(Kocsy et al, , 2001), we measured superoxide radical (O 2…”

Section: Cell Damage and Oxidative Stressmentioning

confidence: 99%

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Cold stress affects antioxidative response and accumulation of medicinally important withanolides in Withania somnifera (L.) Dunal

Mir

Khazir

et al. 2015

Industrial Crops and Products

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Withania somnifera (L.) Dunal (Indian ginseng) is a high value medicinal plant. It synthesizes a large array of biologically active withanolides. In this study, two month old seedlings of AGB002 (wild genotype) and AGB025 (cultivated genotype) of W. somnifera were subjected to cold stress (4 • C) under controlled envi-ronment. Plants were analyzed for three medicinally important secondary metabolites (withanolide A, withanone and withaferin A), lipid peroxidation (MDA), cell injury, superoxide radical (O 2 •− ) accumula-tion and anti-oxidative enzymes activities such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR). Increases in the titers of superoxide anion and MDA were observed from day 1 to day 7 in both genotypes, although the increase on the first day of exposure was significantly higher. Enzymatic activities of SOD, CAT, APX and GR also showed an increasing trend in both genotypes and reached a maximum on day 7 of the cold temperature exposure; however, this increase was higher in AGB002 than AGB025. Withanolide A (WS-1) in the roots of both genotypes sig-nificantly decreased on the first day of cold exposure and then showed a recovery until day 7. WS-1 was not detected in the leaves of either genotype. Withanone (WS-2) content in the leaves also decreased towards the end of the cold period in both genotypes. Cold stress also elicited the accumulation of WS-2 in AGB025, but was not detectable in control seedlings. At maturity, WS-2 was also detected in control plants. Furthermore, a significant increase in the leaf withaferin A (WS-3) content was recorded from day 1 to day 7 of the cold exposure in both the genotypes, suggesting the possible involvement of with-anolides in cold-protection. AGB002 showed comparatively higher accumulation of antioxidant enzymes and selected marker withanolides than AGB025, indicating that AGB002 is better adapted to cold than AGB025. It could be inferred from these observations that cold stress induces bioactive withanolide accu-mulation in W. somnifera as a mechanism for scavenging reactive oxygen species (ROS). These studies also provide an impetus for enhancing the withanolide accumulation in W. somnifera using controlled environment technology.

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Section: Cell Damage and Oxidative Stresssupporting

confidence: 80%

Section: Antioxidant Enzymesmentioning

confidence: 89%

Section: Antioxidant Enzymesmentioning

confidence: 99%

Section: Cell Damage and Oxidative Stressmentioning

confidence: 99%

See 2 more Smart Citations

Cold stress affects antioxidative response and accumulation of medicinally important withanolides in Withania somnifera (L.) Dunal

Mir

Khazir

et al. 2015

Industrial Crops and Products

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“…Both AsA and GSH are required in plants to maintain the intergrity of the photosynthetic membranes and an active AsA-GSH pathway under oxidative stress. 30,31,32 In addition, Kocsy et al 33 suggested that the change of AsA/DHA and GSH/GSSG was more important in cell resistance against ROS compared with AsA or GSH content. Under salt stress decline in AsA/DHA and GSH/GSSG ratios was reversed by SA application.…”

Section: Discussionmentioning

confidence: 99%

Exogenous salicylic acid improves photosynthesis and growth through increase in ascorbate-glutathione metabolism and S assimilation in mustard under salt stress

Nazar

Umar

Khan

2015

Plant Signaling & Behavior

204

114

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Ascorbate (AsA)-glutathione (GSH) cycle metabolism has been regarded as the most important defense mechanism for the resistance of plants under stress. In this study the influence of salicylic acid (SA) was studied on ascorbateglutathione pathway, S-assimilation, photosynthesis and growth of mustard (Brassica juncea L.) plants subjected to 100 mM NaCl. Treatment of SA (0.5 mM) alleviated the negative effects of salt stress and improved photosynthesis and growth through increase in enzymes of ascorbate-glutathione pathway which suggest that SA may participate in the redox balance under salt stress. The increase in leaf sulfur content through higher activity of ATP sulfurylase (ATPS) and serine acetyl transferase (SAT) by SA application was associated with the increased accumulation of glutathione (GSH) and lower levels of oxidative stress. These effects of SA were substantiated by the findings that application of SAanalog, 2,6, dichloro-isonicotinic acid (INA) and 1 mM GSH treatment produced similar results on rubisco, photosynthesis and growth of plants establishing that SA application alleviates the salt-induced decrease in photosynthesis mainly through inducing the enzyme activity of ascorbate-glutathione pathway and increased GSH production. Thus, SA/GSH could be a promising tool for alleviation of salt stress in mustard plants.

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Molecular Bases of Plant Adaptation to Abiotic Stress and Approaches to Enhance Tolerance to Hostile Environments

Coraggio

Tuberosa

2004

Handbook of Plant Biotechnology

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Abiotic stress has become the greatest threat to global food production and security, particularly in developing countries. Progress in increasing yield and its stability under adverse conditions through a direct selection has been hampered by the low heritability of yield, especially under drought. A better knowledge of the molecular and genetic basis of the mechanisms promoting tolerance to abiotic stress will enhance our capacity to more appropriately manipulate the genes limiting crop yield under hostile environments. This review describes and critically analyses the major advances in our understanding of the complex mechanisms underlying the adaptive response of the plant to abiotic stress, starting with the perception of the stress at the cellular level, its transduction through a cascade of signalling molecules and ending with the induction of genes whose products mitigate the harmful effects of the stress. Molecular markers and the ‘omics’ platforms (e.g., transcriptomics, proteomics, metabolomics etc.) offer unprecedented opportunities to identify and clone the genes governing plant adaptation to environmental stress and limiting yield under such conditions. The dissection of the genetic basis of quantitative traits into their single components, the so‐called QTLs (quantitative trait loci), allows us to deploy marker‐assisted selection (MAS) for enhancing crop performance under adverse conditions. Additional opportunities are also offered by genetic engineering, particularly through the expression of transcription factors regulating a suite of genes whose product is crucial to mitigate the effects of stress. Although the applicative results so far achieved with non‐conventional approaches have been limited, their integration with conventional breeding methodologies and other interdisciplinary approaches will greatly enhance our capacity to more effectively tailor crop genome to withstand harsh environmental conditions and to improve their sustainability under marginal conditions.

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Role of glutathione in adaptation and signalling during chilling and cold acclimation in plants

Cited by 236 publications

References 57 publications

Cold stress affects antioxidative response and accumulation of medicinally important withanolides in Withania somnifera (L.) Dunal

Cold stress affects antioxidative response and accumulation of medicinally important withanolides in Withania somnifera (L.) Dunal

Exogenous salicylic acid improves photosynthesis and growth through increase in ascorbate-glutathione metabolism and S assimilation in mustard under salt stress

Molecular Bases of Plant Adaptation to Abiotic Stress and Approaches to Enhance Tolerance to Hostile Environments

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