Role of Glutathione-S-Transferase Family Genes in Male Infertility

Колесникова, Л. И.; Курашова, Н. А.; Bairova, T. A.; Долгих, М. И.; Ершова, О. А.; Dashiev, B. G.; Korytov, L. I.; Koroleva, N. V.

doi:10.1007/s10517-017-3869-9

Cited by 18 publications

(17 citation statements)

References 10 publications

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“…Первичные продукты процесса ПОЛ, как правило, являются очень неустойчивыми веществами и легко подвергаются дальнейшим превращениям с образованием более стабильных компонентов окисления: альдегидов, кетонов, низкомолекулярных кислот, -вследствие чего отмечается широкий диапазон их изменений. Снижение значений ТБК-АП у мужчин с олигозооспермией может свидетельствовать об активации ферментативного звена антиоксидантной защиты, в частности ферментов супероксиддисмутазы, окисленного и восстановленного глутатиона [1,17,19].…”

Section: результаты и обсуждениеunclassified

“…На сегодняшний день бесплодный брак представляет собой очень сложную и серьёзную медико-социальную проблему [1,2,3,4]. Несмотря на совершенствование клинико-лабораторных обследований и методов и внедрение в широкую клиническую практику вспомогательных репродуктивных технологий, частота бесплодия в браке колеблется в широких пределах (7-28 %) и не имеет тенденции к снижению как в России, так и за рубежом [5].…”

Section: Introductionunclassified

“…Для преодоления этих нежелательных последствий АФК естественным образом стабилизируются компонентами антиоксидантной защиты организма. Однако в случаях, когда наблюдается избыточное количество АФК и система антиоксидантной защиты не справляется, развивается состояние ОС [1,9,10]. Сперматозоиды особенно уязвимы для ОС, поскольку не обладают необходимыми цитоплазматическими антиоксидантными системами восстановления.…”

Section: Introductionunclassified

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Parameters of Lipid Peroxidation and Antioxidant Protection in Men with Pathological Spermogram Variants

Курашова

Кудеярова²,

Кузнецова

2019

Acta biomedica scientifica

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Background.Today infertile marriage is not only a serious medical, but also a socio-demographic and economic problem. Male factor contributes averagely to half of the cases of the disease in couples. Such factors as high levels of reactive oxygen species and oxidative stress have been reported to compromise the process of spermatogenesis and sperm function in men. Oxidative stress is a significant risk factor for male infertility. A pro-oxidant testicular environment may alter the expression profile of functional sperm proteins and result in poor sperm quality.Aims.To study the characteristics of the intensity of the processes of lipoperoxidation and antioxidant status in the ejaculate of men with different variants of spermograms.Materials and methods.We examined 69 men with primary infertility and 155 fertile men. The content of lipid peroxidation components and antioxidant protection was determined by spectrophotometric method.Results.The results of the study in men with infertility and asthenozoospermia showed decreased total antioxidant activity of sperm by 50 % and α-tocopherol by 52 %, and in men with infertility and oligozoospermia, decreased total antioxidant activity of sperm by 47 % and α-tocopherol by 41 %.Conclusions.The analysis indicates a change in the parameters of the system of lipid peroxidation – antioxidant defense system and confirms the development of oxidative stress in them. Depending on the pathological state of the ejaculate in men of reproductive age, lipid peroxidation processes have their own characteristics. In men with oligozoospermia, peroxidation processes occur more intensively. Activation of lipid peroxidation – antioxidant defense system processes can be both a consequence and a cause of various metabolic changes in the human body.

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Section: результаты и обсуждениеunclassified

Section: Introductionunclassified

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Parameters of Lipid Peroxidation and Antioxidant Protection in Men with Pathological Spermogram Variants

Курашова

Кудеярова²,

Кузнецова

2019

Acta biomedica scientifica

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“…Currently, infertile couples represent a very complex and serious medical and social problem. (1)(2)(3)(4) Despite the improvement of clinical-laboratory examinations and methods, and the introduction of auxiliary reproductive technologies into the wide clinical practice, the incidence of infertility in the marriage varies widely (7-28%) and does not tend to decrease. (5) In 40%-60% of cases, the reason for the absence of children in the family is the male factor.…”

Section: Introductionmentioning

confidence: 99%

“…(4)(5)(6)(7) According to the literature, many factors affect the quality of the ejaculate, including an unfavorable ecological situation, inadequate and unbalanced nutrition, smoking, alcohol, inflammatory diseases of the genitourinary system, varicocele, and food products. (1,4) OS has been identified as one of the many mediators of male infertility by causing sperm dysfunction. Active forms *Corresponding author: Nadezhda A. Kurashova, PhD, ScD.…”

Section: Introductionmentioning

confidence: 99%

The Processes of Lipoperoxidation and Antioxidant Protection in Men with Different Variants of Spermograms

Курашова¹,

Dashiev²,

Долгих³

et al. 2019

IJBM

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Background: Currently, infertile couples represent a very complex and serious medical and social problem. In 40%-60% of cases, the reason for the absence of children in the family is the male factor. Oxidative stress (OS) has been identified as one of the many mediators of male infertility by causing sperm dysfunction. The purpose of this study was to identify the characteristics of the intensity indices of lipid peroxidation (LPO) and antioxidant system (AOS) in the ejaculate of men with different spermogram variants. Materials and Methods: We performed a retrospective analysis of the results of examinations of 69 men of infertile couples with various disorders of the ejaculate and 155 healthy men with proven fertility. In the semen of the examined men, the level of CD (conjugated dienes), TBARS (thiobarbituric acid reactive substances), α-tocopherol, retinol and total antioxidant activity (AOA) was evaluated by spectrophotometric method. Results: We found that the antioxidant protection system in patients with asthenozoospermia was characterized by a decrease in the level of total AOA in the semen by 50% and the α-tocopherol concentration by 52%, compared to healthy men in the control group. In patients with oligozoospermia, an increase in the CD concentration by 39% and a decrease in the TBARS concentration by 26% was found; the level of total AOA decreased by 37% and the concentration of α-tocopherol by 41%, compared to healthy men. Conclusion: In general, the analysis of the obtained data of the survey of men with different variants of spermograms indicates a change in the parameters of the LPO-AOS and confirms the OS development. Thus, it can be noted that depending on the pathological state of the ejaculate in men of reproductive age, LPO processes have their own characteristics, and in men with oligozoospermia, LPO processes occur more intensively. Activation of LPO-AOS processes can be both a consequence and a cause of various metabolic changes in the human body.

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Glutathione S‐transferase kappa 1 is positively related with sperm quality of porcine sperm

Zeng

Huang

et al. 2021

Molecular Reproduction Devel

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The glutathione S-transferase (GST) superfamily members play an important role in the male reproductive tract and sperm physiology. However, the expression profiles of some members of this protein family and their effect on sperm quality remain unclear. In this study, we found that GST kappa 1 (GSTK1) encoded protein is abundant in the testes and capacitated sperm acrosome. Western blot analysis revealed that the decreased abundance of GSTK1 was observed in low motile spermatozoa; moreover, GSTK1 expression decreased in sperm stored at 17°C under a long preservation time. In vitro analyses revealed that GSTK1 had no significant effect on sperm motility, capacitation, or acrosome reaction. Notably, after capacitated sperm were incubated with 4 and 8 μg/ml anti-GSTK1 antibodies, the fertilization rate significantly decreased in vitro fertilization assay. The current study demonstrates that GSTK1 is correlated with sperm quality and is a promising marker for the assessment of sperm quality and provides a basis for understanding the potential molecular mechanism for targeting pathogenic factors in male infertility.

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Role of Glutathione-S-Transferase Family Genes in Male Infertility

Cited by 18 publications

References 10 publications

Parameters of Lipid Peroxidation and Antioxidant Protection in Men with Pathological Spermogram Variants

Parameters of Lipid Peroxidation and Antioxidant Protection in Men with Pathological Spermogram Variants

The Processes of Lipoperoxidation and Antioxidant Protection in Men with Different Variants of Spermograms

Glutathione S‐transferase kappa 1 is positively related with sperm quality of porcine sperm

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