Role of glycerol 3-phosphate dehydrogenase in glyceride metabolism. Effect of diet on enzyme activities in chicken liver

Harding, Joseph W.; Pyeritz, E A; Em, Copeland; White, Harold B.

doi:10.1042/bj1460223

Cited by 67 publications

(45 citation statements)

References 32 publications

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“…Glycerol-3-phosphate dehydrogenase (Gpd) plays a major role in lipid biosynthesis. Through the reduction of dihydroxyacetone phosphate into glycerol 3-phosphate, Gpd allows for the dephosphorylation of glycerol 3-phosphate into glycerol (Harding et al, 1975). The increase in Gpd1 expression is consistent with the increased number of lipid droplets observed in the ultrastructural analysis.…”

Section: Proteins Related To Liver Metabolismsupporting

confidence: 67%

A proteomic study on liver impairment in rat pups induced by maternal microcystin-LR exposure

Zhao

Xie

Chen

et al. 2016

Environmental Pollution

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Section: Proteins Related To Liver Metabolismsupporting

confidence: 67%

A proteomic study on liver impairment in rat pups induced by maternal microcystin-LR exposure

Zhao

Xie

Chen

et al. 2016

Environmental Pollution

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“…Gpd1 encodes glycerol-3-phosphate dehydrogenase 1 that has an important role in the synthesis of triacylglycerol and in the transport of reducing equivalents from the cytosol to mitochondria. 58 Hk2 encodes hexokinase 2 that phosphorylates glucose to produce glucose-6-phosphate, the preliminary step of intracellular glucose metabolism. 59 Pklr encodes pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP.…”

Section: Evidence For Liver Programmingmentioning

confidence: 99%

Effect of intrauterine growth retardation on liver and long-term metabolic risk

Cianfarani

Agostoni

Bedogni³

et al. 2012

Int J Obes

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Intrauterine growth retardation predisposes toward long-term morbidity from type 2 diabetes and cardiovascular disease. To explain this association, the concept of programming was introduced to indicate a process whereby a stimulus or insult at a critical period of development has lasting or lifelong consequences on key endocrine and metabolic pathways. Subtle changes in cell composition of tissues, induced by suboptimal conditions in utero, can influence postnatal physiological functions. There is increasing evidence, suggesting that liver may represent one of the candidate organs targeted by programming, undergoing structural, functional and epigenetic changes following exposure to an unfavorable intrauterine environment. The aim of this review is to provide insights into the molecular mechanisms underlying liver programming that contribute to increase the cardiometabolic risk in subjects with intrauterine growth restriction.

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“…Glycerol-3-phosphate dehydrogenase activity was determined in adipose tissue-soluble fraction as described by Harding et al (15). The decrease in absorbance at 340 nm was followed for 5 min.…”

Section: Enzyme Assaysmentioning

confidence: 99%

Differences in transport of fatty acids and expression of fatty acid transporting proteins in adipose tissue of obese black and white women

Bower

Davis

Hao

et al. 2006

American Journal of Physiology-Endocrinology and Metabolism

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. Differences in transport of fatty acids and expression of fatty acid transporting proteins in adipose tissue of obese black and white women. Am J Physiol Endocrinol Metab 290: E87-E91, 2006; doi:10.1152/ajpendo.00194.2005.-We have reported that the rate of de novo triglyceride (TG) synthesis by omental, but not subcutaneous, adipose tissue was higher in African-American women (AAW) than in Caucasian women (CAW). The purpose of this study was to explore the potential mechanisms underlying this increase. Toward that end, we determined the activities of key enzymes in the pathway of TG synthesis, the rates of uptake of fatty acids by adipocytes, mRNA and protein levels of the fatty acid-transporting proteins FAT/CD36 and FATP, and mRNA and protein levels of PPAR␥ in omental fat of AAW and CAW. The results showed 1) no difference in the activity of phosphofructokinase, glycerol-3-phosphate dehydrogenase, or diacylglycerol acyltransferase; 2) a higher rate of fatty acid uptake by adipocytes of the AAW; 3) an increase in the mRNA and protein levels of CD36 and FATP4 in the fat of the AAW; and 4) an increase in the mRNA and protein levels of PPAR␥, which can stimulate the expression of CD36 and FATP. These results suggest that the increase in the transport of fatty acid, which is mediated by the overexpression of the transport proteins in the omental adipose tissue of the AAW, might contribute to the higher prevalence of obesity in AAW.ethnicity; lipid synthesis; substrate transport; omental fat; subcutaneous fat OBESITY IS MORE PROMINENT among African-American women (AAW) than among Caucasian women (CAW) (21). Nearly 50% of AAW are overweight compared with 33% of CAW (20). Obese AAW suffer more from obesity-related comorbidities than CAW (12,25,26). Among older women, the incidence of obesity in AAW is almost twice that of CAW (25). These data suggest that there are biological differences that contribute to the obesity of AAW that have not been defined.We have reported several differences in lipid and lipoprotein metabolism between 16,18,23,24,27,28). In a previous study (8), we reported differences in de novo triglyceride (TG) synthesis by adipose tissue preparations from AAW and CAW. We found no differences in the rate of synthesis of either TG or diglyceride (DG) in subcutaneous adipose tissue of the two groups of women. However, the rate of TG synthesis in omental adipose tissue was higher in AAW than in CAW. This increase was not due to differences in cell size or rates of reesterification (8).This study was initiated to examine the underlying causes of the higher rate of fatty acid incorporation into TG in adipose tissue of the AAW. This higher rate could be due to a higher expression of the enzymes of the pathway of TG synthesis, a higher rate of substrate uptake, or both. In the current study, we sought to determine whether there were differences in these two processes between the AAW and the CAW. To that end, we measured the activities of several key enzymes of the pathway of TG synthesis, the rate of upta...

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Role of glycerol 3-phosphate dehydrogenase in glyceride metabolism. Effect of diet on enzyme activities in chicken liver

Cited by 67 publications

References 32 publications

A proteomic study on liver impairment in rat pups induced by maternal microcystin-LR exposure

A proteomic study on liver impairment in rat pups induced by maternal microcystin-LR exposure

Effect of intrauterine growth retardation on liver and long-term metabolic risk

Differences in transport of fatty acids and expression of fatty acid transporting proteins in adipose tissue of obese black and white women

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