Role of Glycoproteins Isolated from &lt;i&gt;Epicoccum purpurascens&lt;/i&gt; in Host-Pathogen Interaction

Kukreja, Neetu; Arora, Naveen; Singh, Bhanu; Das, Hasi R.; Sridhara, S.

doi:10.1159/000103378

Cited by 4 publications

(2 citation statements)

References 46 publications

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“…ratti [16]. Epi p 1 activates the mannose binding lectin‐mediated complement pathway and may play a role in the initiation of fungal infection [19]. The present study was aimed to assess the role of proteolytic activity of Epi p 1 in inducing allergic inflammation in mice.…”

Section: Introductionmentioning

confidence: 98%

Effect of proteolytic activity ofEpicoccum purpurascensmajor allergen, Epi p 1 in allergic inflammation

Kukreja

Sridhara

Singh

et al. 2008

Clinical and Experimental Immunology

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SummaryEnzymes play an important role in inducing airway inflammation, but knowledge is limited to few proteins. This study was carried out to assess the role of Epi p 1, a serine protease of Epicoccum purpurascens, in inducing allergy and inflammation in a murine model. Balb/c mice were sensitized with Epi p 1 active protease (EAP) or Epicoccum extract. Subsequently, Epi p 1 sensitized mice were boosted on day 14 with EAP or inactivated protease (EIAP). Three intranasal challenges were given and mice were killed to obtain blood, bronchoalveolar lavage fluid (BALF), spleen and lung tissues. Cellular airways infiltration, immunoglobulin E (Ig)E titres and cytokine levels in BALF and splenocyte culture supernatant were compared. Mice immunized with EAP had higher Epi p 1-specific serum IgE and IgG1 than EIAP immunized mice (P < 0·01). There was a twofold difference in the number of eosinophils in BALF of EAP mice and EIAP mice (P < 0·01). A similar trend was recorded for eosinophil peroxidase activity (P < 0·05), indicating the role of proteolytic activity in inducing inflammation. Further, lung histology revealed increased leucocyte infiltration and airway narrowing, with higher inflammation scores in the EAP group than in the EIAP group. The lungs of EAP mice showed increased mucus and goblet cell metaplasia. Interleukin (IL)-4 and IL-5 levels were higher in BALF and splenocyte culture supernatant of EAP mice than in EIAP mice (P < 0·05), indicating a T helper 2 response. Proteolytic activity of Epi p 1 plays an important role in inducing allergic inflammation. The enzymatically inactive form may be investigated for immunotherapy.

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Section: Introductionmentioning

confidence: 98%

Effect of proteolytic activity ofEpicoccum purpurascensmajor allergen, Epi p 1 in allergic inflammation

Kukreja

Sridhara

Singh

et al. 2008

Clinical and Experimental Immunology

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“…Fax: +27-21-8093260. surfaces has survival value and may be a requirement for colonisation (Peñalver et al, 1996;Kukreja et al, 2007;Tronchin et al, 2008). This deposition of spores may impact on health since these spores may result in respiratory tract infection (Supparatpinyo et al, 1994;Denning et al, 2006;Bellanger et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Hyphomycetous fungi spore release induced by air currents and aqueous solution

Hart¹,

Calitz²,

Botha³

2014

Afr. J. Microbiol. Res.

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Hyphomycetous fungi originating from South Africa were morphologically characterised and ascribed to the genera Acremonium, Aspergillus and Penicillium, respectively. The primary means of spore dispersal employed by these isolates was investigated by quantifying colony forming units released into the air and into an aqueous solution. Measurement of spore liberation during humid aeration, revealed significant (P < 0.0001) differences among the hyphomycetous taxa investigated. Isolates of the genus Penicillium were more successful in releasing their spores than the Aspergilli and the Acremonium isolate. Spore liberation during desiccated aeration also showed a significant (P < 0.0001) difference between the respective isolates. Overall, isolates belonging to the genus Penicillium released more viable spores than Aspergillus spp., which in turn released more spores than Acremonium. In support of the theory that splashing rain may dislodge and disperse microfungal propagules, washing respective cultures with physiological salt solution resulted in an immediate massive spore release. However, the taxa investigated did not differ. These differences in airborne spore release that were observed between the hyphomycetous genera may be a result of different strategies to disperse their spores in nature. This phenomenon should be investigated further in future and the challenge now is to find correlations between the conidiophore morphology of each fungus and characteristics of their niche.

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The Black Gap: Understanding the Potential Roles of Black Fungal-Derived Enzymes in Malting and Brewing Quality: A Review

Bretträger,

Sacher,

Gastl

et al. 2023

Journal of the American Society of Brewing Chemists

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Role of Glycoproteins Isolated from <i>Epicoccum purpurascens</i> in Host-Pathogen Interaction

Cited by 4 publications

References 46 publications

Effect of proteolytic activity ofEpicoccum purpurascensmajor allergen, Epi p 1 in allergic inflammation

Effect of proteolytic activity ofEpicoccum purpurascensmajor allergen, Epi p 1 in allergic inflammation

Hyphomycetous fungi spore release induced by air currents and aqueous solution

The Black Gap: Understanding the Potential Roles of Black Fungal-Derived Enzymes in Malting and Brewing Quality: A Review

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