Role of Golgi Apparatus in Sorogenesis by the Cellular Slime Mold
            <i>Fonticula alba</i>

Deasey, Mary C.; Olive, Lindsay S.

doi:10.1126/science.213.4507.561

Cited by 10 publications

(11 citation statements)

References 9 publications

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“…Parvularia and Fonticula are both exclusively bacterivorous and part of nanoplankton, the first never reaches more than 6 µm [20] and the latter no more than 12 µm [5,39] in size. Fonticula alba, which seems to evolve faster than other nucleariids (see branch lengths in figures 1 and 2), grows better in agar plates than in liquid medium (D. López-Escardó 2017, personal communication), and uses its mucilaginous coat to aggregate cells and form fruiting bodies [74]. Hence, F. alba looks more adapted to soil environments than to the water column preferred by other nucleariids.…”

Section: (D) Evolutionary Implicationsmentioning

confidence: 98%

Combined cultivation and single-cell approaches to the phylogenomics of nucleariid amoebae, close relatives of fungi

Galindo

Torruella

Moreira

et al. 2019

Phil. Trans. R. Soc. B

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Nucleariid amoebae (Opisthokonta) have been known since the nineteenth century but their diversity and evolutionary history remain poorly understood. To overcome this limitation, we have obtained genomic and transcriptomic data from three Nuclearia , two Pompholyxophrys and one Lithocolla species using traditional culturing and single-cell genome (SCG) and single-cell transcriptome amplification methods. The phylogeny of the complete 18S rRNA sequences of Pompholyxophrys and Lithocolla confirmed their suggested evolutionary relatedness to nucleariid amoebae, although with moderate support for internal splits. SCG amplification techniques also led to the identification of probable bacterial endosymbionts belonging to Chlamydiales and Rickettsiales in Pompholyxophrys . To improve the phylogenetic framework of nucleariids, we carried out phylogenomic analyses based on two datasets of, respectively, 264 conserved proteins and 74 single-copy protein domains. We obtained full support for the monophyly of the nucleariid amoebae, which comprise two major clades: (i) Parvularia–Fonticula and (ii) Nuclearia with the scaled genera Pompholyxophrys and Lithocolla . Based on these findings, the evolution of some traits of the earliest-diverging lineage of Holomycota can be inferred. Our results suggest that the last common ancestor of nucleariids was a freshwater, bacterivorous, non-flagellated filose and mucilaginous amoeba. From the ancestor, two groups evolved to reach smaller ( Parvularia–Fonticula ) and larger ( Nuclearia and related scaled genera) cell sizes, leading to different ecological specialization. The Lithocolla + Pompholyxophrys clade developed exogenous or endogenous cell coverings from a Nuclearia -like ancestor. This article is part of a discussion meeting issue ‘Single cell ecology’.

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Section: (D) Evolutionary Implicationsmentioning

confidence: 98%

Combined cultivation and single-cell approaches to the phylogenomics of nucleariid amoebae, close relatives of fungi

Galindo

Torruella

Moreira

et al. 2019

Phil. Trans. R. Soc. B

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“…Fonticula alba (Fig. 3A-F) was isolated once from dog dung (Deasey and Olive 1981;Worley et al 1979). It is a small amoeba, spherical to elongated (5 to 12 µm), with filose or monopodial lobose pseudopodia (Fig.…”

Section: Morphology and Life Historymentioning

confidence: 99%

On the Biology, Diversity and Evolution of Nucleariid Amoebae (Amorphea, Obazoa, Opisthokonta1

Gabaldón

Völcker²,

Torruella

2022

Protist

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“…Although this increased density of labeling in the blebs is small, it is statistically significant . Such blebs might correspond to structures that have been observed morphologically at the nuclear envelope (17)(18)(19) and at the RER (20), which have been postulated to represent so-called "transitional elements" on the pathway from the RER to the Golgi complex . Such transitional elements might then pinch off to form vesicles to deliver the G-protein to the Golgi complex .…”

Section: Events In the Rough Endoplasmic Reticulummentioning

confidence: 99%

“…Little is known about the ability of these pore complexes to restrict the passage of integral membrane proteins from the outer to the inner nuclear membrane . Artifacts have plagued both immunocyto- BERGMANN chemical and biochemical approaches to this problem (18) .…”

Section: Contiguity Of the Inner And Outer Nuclear Membranesmentioning

confidence: 99%

Immunoelectron microscopic studies of the intracellular transport of the membrane glycoprotein (G) of vesicular stomatitis virus in infected Chinese hamster ovary cells.

Bergmann¹,

Singer²

1983

The Journal of Cell Biology

145

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An immunoelectron microscopic study was undertaken to survey the intracellular pathway taken by the integral membrane protein (G-protein) of vesicular stomatitis virus from its site of synthesis in the rough endoplasmic reticulum to the plasma membrane of virusinfected Chinese hamster ovary cells. Intracellular transport of the G-protein was synchronized by using a temperature-sensitive mutant of the virus (0-45) . At the nonpermissive temperature (39 .8°C), the G-protein is synthesized in the cell infected with 0-45, but does not leave the rough endoplasmic reticulum . Upon shifting the temperature to 32°C, the G-protein moves by stages to the plasma membrane . Ultrathin frozen sections of 0-45-infected cells were prepared and indirectly immunolabeied for the G-protein at different times after the temperature shift. By 3 min, the G-protein was seen at high density in saccules at one face of the Golgi apparatus . No large accumulation of G-protein-containing vesicles were observed near this entry face, but a few 50-70-mm electron-dense vesicular structures labeled for G-protein were observed that might be transfer vesicles between the rough endoplasmic reticulum and the Golgi complex . At blebbed sites on the nuclear envelope at these early times there was a suggestion that the G-protein was concentrated, these sites perhaps serving as some of the transitional elements for subsequent transfer of the G-protein from the rough endoplasmic reticulum to the Golgi complex . By 3 min after its initial asymmetric entry into the Golgi complex, the G-protein was uniformly distributed throughout all the saccules of the complex . At later times, after the G-protein left the Golgi complex and was on its way to the plasma membrane, a new class of G-protein-containing vesicles of -200-nm diameter was observed that are probably involved in this stage of the transport process . These data are discussed, and the further prospects of this experimental approach are assessed .Integral proteins ofthe plasma membrane are of great importance in cellular physiology . They play key roles in cell-cell interactions, intercellular communication, hormone reception and signal transmission, ion and metabolite transport, and many other functions. Despite the importance of these proteins, little is known yet about their biogenesis and the molecular details of their intracellular transport to the cell surface . While the presence ofseveral integral protein enzymes and receptors of the plasma membrane in the Golgi apparatus (1-4) has shown that organelle to be involved in the intracellular traffic of these proteins, the traffic from within the cell to the outside has not been clearly differentiated from the THE JOURNAL OF CELL BIOLOGY " VOLUME 97 December 1983 1777-1787 0 The Rockefeller University Press -0021-9525/83/12/1777/11 $1 .00 traffic in the reverse direction, and other elements of the pathway to the cell exterior are unknown . In delineating the details of these intracellular pathways there are limits to the information that can...

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Role of Golgi Apparatus in Sorogenesis by the Cellular Slime Mold Fonticula alba

Abstract: Aggregating cells of the cellular slime mold Fonticula alba form a volcano-shaped fruiting structure which at maturity bears its spores apically in a globose mucous mass. Numerous dictyosomes forming in the sorogenic cells are involved in the accumulation and deposition of stalk material.

Cited by 10 publications

References 9 publications

Combined cultivation and single-cell approaches to the phylogenomics of nucleariid amoebae, close relatives of fungi

Combined cultivation and single-cell approaches to the phylogenomics of nucleariid amoebae, close relatives of fungi

On the Biology, Diversity and Evolution of Nucleariid Amoebae (Amorphea, Obazoa, Opisthokonta1

Immunoelectron microscopic studies of the intracellular transport of the membrane glycoprotein (G) of vesicular stomatitis virus in infected Chinese hamster ovary cells.

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