Role of Gram stain in microbiological laboratories with limited resources

Abstract

Despite recent advances in automated laboratory systems, the Gram stain is still one of the most important tools in clinical microbiology, especially for laboratories with limited resources. With the development of molecular techniques and rapid immunological methods, it now seems that less attention has been paid to the Gram stain compared with before. This review describes the role that the Gram stain plays in the rapid presumptive diagnosis, quality evaluation of clinical samples, and antimicrobial management. Some discussion is also presented in relation to the interpretation of Gram stain and the quality control of Gram staining method.

How to obtain and isolate equine sternal bone marrow mononuclear cells with limited resources

“…The use of laminar flow hood to reduce risk of contamination is advised, however it does not ensure septic complications will not happen. Besides, in this project Gram staining, an important tool in microbiology (Beveridge 2001, Su RJ andPei 2011), resulted negative for all samples suggesting the absence of Gram + and Gram -bacterial contamination. Gram staining plays an important role in the rapid presumptive diagnosis, quality evaluation and antimicrobial management of clinical samples in facilities were no cell culture is available (Su R-J and Pei 2011).…”