Role of Growth Temperature in Freeze-Thaw Tolerance of
            <i>Listeria</i>
            spp

Azizoglu, Reha Onur; Osborne, Jason A.; Wilson, Sandra L.; Kathariou, Sophia

doi:10.1128/aem.00458-09

Cited by 39 publications

(38 citation statements)

References 43 publications

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“…The altitudinal gradient of temperature may affect the bacterial community since the chance of freezing on the glacier increases with altitude. Anti-freezing resistance is likely to differ among bacteria (2,49). Thus, the upper-zone type may include bacteria that are more resistant to freezing on the glacier.…”

Section: Discussionmentioning

confidence: 99%

Altitudinal Changes in a Bacterial Community on Gulkana Glacier in Alaska

et al. 2010

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To clarify altitudinal changes in the bacterial community on Gulkana Glacier in Alaska, we analyzed bacterial 16S rRNA gene by low-cycle PCR amplification, denaturing gradient gel electrophoresis (DGGE), and culturing in a snowmelt medium at 4°C. Low-cycle PCR-based cloning revealed the presence of 100 bacterial OTUs; however, 41 OTUs were identified only in a single clone, suggesting that their abundance was limited because of difficulty in predominating on the glacier. In contrast, 17 major OTUs accounted for 57-87% of the clone library at each site, suggesting that they accounted for the major part of the bacteria on the glacier. In addition, five of the 17 OTUs were included in the 21 OTUs cultured in the snowmelt medium. Based on the dominant phylotypes and DGGE results, the bacterial community on the glacier could be divided into three types, corresponding to the snow-covered, snow-and icecovered, and bare-ice areas of the glacier. Our results suggest that a relatively limited number of bacteria predominate and that each phylotype is adapted to a distinct set of conditions on the glacier.

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Section: Discussionmentioning

confidence: 99%

Altitudinal Changes in a Bacterial Community on Gulkana Glacier in Alaska

et al. 2010

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“…ROA3 and the wild-type parental strain F2365 were grown in TSBYE medium at 25°C (48 h) or 37°C (36 h), and samples (1.5 ml) were subjected to 18 cycles of repeated freezing (Ϫ20°C) and thawing, as described previously (1). Cryotolerance was assessed by enumeration of cells surviving following freezing and thawing, as described previously (1).…”

Section: Methodsmentioning

confidence: 99%

Temperature-Dependent Requirement for Catalase in Aerobic Growth of Listeria monocytogenes F2365

Azizoglu

Kathariou

2010

Appl Environ Microbiol

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Listeria monocytogenes is a Gram-positive, psychrotrophic, facultative intracellular food-borne pathogen responsible for severe illness (listeriosis). The bacteria can grow in a wide range of temperatures (1 to 45°C), and low-temperature growth contributes to the food safety hazards associated with contamination of readyto-eat foods with this pathogen. To assess the impact of oxidative stress responses on the ability of L. monocytogenes to grow at low temperatures and to tolerate repeated freeze-thaw stress (cryotolerance), we generated and characterized a catalase-deficient mutant of L. monocytogenes F2365 harboring a mariner-based transposon insertion in the catalase gene (kat). When grown aerobically on blood-free solid medium, the kat mutant exhibited impaired growth, with the extent of impairment increasing with decreasing temperature, and no growth was detected at 4°C. Aerobic growth in liquid was impaired at 4°C, especially under aeration, but not at higher temperatures (10, 25, or 37°C). Genetic complementation of the mutant with the intact kat restored normal growth, confirming that inactivation of this gene was responsible for the growth impairment. In spite of the expected impact of oxidative stress responses on cryotolerance, cryotolerance of the kat mutant was not affected.

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“…Rapid and reliable detection methods of this pathogenic, toxin-producing bacterium are required since it is able to survive and grow at low temperatures [22] and because the mortality rate for infected individuals is much higher than for other common foodborne pathogens [23–27]. …”

Section: Introductionmentioning

confidence: 99%

An Internalin A Probe-Based Genosensor forListeria monocytogenesDetection and Differentiation

Bifulco

Ingianni

Pompei

2013

BioMed Research International

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Internalin A (InlA), a protein required for Listeria monocytogenes virulence, is encoded by the inlA gene, which is only found in pathogenic strains of this genus. One of the best ways to detect and confirm the pathogenicity of the strain is the detection of one of the virulence factors produced by the microorganism. This paper focuses on the design of an electrochemical genosensor used to detect the inlA gene in Listeria strains without labelling the target DNA. The electrochemical sensor was obtained by immobilising an inlA gene probe (single-stranded oligonucleotide) on the surfaces of screen-printed gold electrodes (Au-SPEs) by means of a mercaptan-activated self-assembled monolayer (SAM). The hybridisation reaction occurring on the electrode surface was electrochemically transduced by differential pulse voltammetry (DPV) using methylene blue (MB) as an indicator. The covalently immobilised single-stranded DNA was able to selectively hybridise to its complementary DNA sequences in solution to form double-stranded DNA on the gold surface. A significant decrease of the peak current of the voltammogram (DPV) upon hybridisation of immobilised ssDNA was recorded. Whole DNA samples of L. monocytogenes strains could be discriminated from other nonpathogenic Listeria species DNA with the inlA gene DNA probe genosensor.

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Role of Growth Temperature in Freeze-Thaw Tolerance of Listeria spp

Cited by 39 publications

References 43 publications

Altitudinal Changes in a Bacterial Community on Gulkana Glacier in Alaska

Altitudinal Changes in a Bacterial Community on Gulkana Glacier in Alaska

Temperature-Dependent Requirement for Catalase in Aerobic Growth of Listeria monocytogenes F2365

An Internalin A Probe-Based Genosensor forListeria monocytogenesDetection and Differentiation

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