2016
DOI: 10.1016/j.exppara.2016.07.012
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Role of H+-pyrophosphatase activity in the regulation of intracellular pH in a scuticociliate parasite of turbot: Physiological effects

Abstract: The scuticociliatosis is a very serious disease that affects the cultured turbot, and whose causal agent is the anphizoic and marine euryhaline ciliate Philasterides dicentrarchi. Several protozoans possess acidic organelles that contain high concentrations of pyrophosphate (PPi), Ca(2+) and other elements with essential roles in vesicular trafficking, pH homeostasis and osmoregulation. P. dicentrarchi possesses a pyrophosphatase (H(+)-PPase) that pumps H(+) through the membranes of vacuolar and alveolar sacs.… Show more

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Cited by 6 publications
(9 citation statements)
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“…For quantification of the expression of the TMPT2A by the trophonts incubated with turbot immune serum for 30 min and 6h, a FELISA was conducted as previously described [14]. Ciliate lysate (CL), prepared as previously described [20], was used as antigen in the assay.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…For quantification of the expression of the TMPT2A by the trophonts incubated with turbot immune serum for 30 min and 6h, a FELISA was conducted as previously described [14]. Ciliate lysate (CL), prepared as previously described [20], was used as antigen in the assay.…”
Section: Methodsmentioning
confidence: 99%
“…Philasterides dicentrarchi is an amphizoic scuticociliate, originally free-living, which under certain conditions can be transformed into an opportunistic histiophagous parasite in cultivated flat fish, causing a serious disease called scuticociliatosis and producing high mortality rates [11,12]. In order to produce the parasitic phase, the ciliate must develop various strategies of biochemical adaptation to its new habitat [13,14]. In addition, it must evade attack by the fish immune system, especially by lysis induced by soluble factors in the serum, such as complement.…”
Section: Introductionmentioning
confidence: 99%
“…For AOX immunolocalization in trophonts, an IIF immunoassay was performed, as previously described 46 . Briefly, ciliates were fixed in a solution of 4% formaldehyde in PBS, permeabilized in a solution containing 0.3% Triton X-100 in PBS and blocked with a solution of 1% BSA.…”
Section: Indirect Immunofluorescence (Iif)mentioning
confidence: 99%
“…For quantification of TMPT2A expression by the trophonts incubated with turbot immune serum for 30 min and 6h, a FELISA was conducted as previously described [14]. Ciliate lysate (CL), prepared as previously described [20], was used as antigen in the assay.…”
Section: Fluorescent Enzyme-linked Immunosorbent Assay (Felisa)mentioning
confidence: 99%
“…Philasterides dicentrarchi is an amphizoic scuticociliate, originally free-living, but which under certain conditions can be transformed into an opportunistic histiophagous parasite in cultivated flat fish, causing a serious disease called scuticociliatosis and causing high mortality rates [11,12]. In order to produce the parasitic phase, the ciliate must develop various strategies of biochemical adaptation to its new habitat [13,14]. In addition, it must evade attack by the fish immune system, especially by lysis induced by soluble factors in the serum, such as complement.…”
Section: Introductionmentioning
confidence: 99%