Role of H<sub>2</sub>O<sub>2</sub>in pea seed germination

Barba‐Espín, Gregorio; Hernández, José Antonio; Díaz‐Vivancos, Pedro

doi:10.4161/psb.18881

Cited by 83 publications

(65 citation statements)

References 14 publications

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“…Although ROS have been considered as detrimental to seeds, advances in plant physiology evaluated them as messengers of various signal transduction pathways in plants. ROS are suggested as being beneicial for seed germination, seedling growth, protection against pathogens and controlling the cell redox status [28][29][30][31][32][33][34][35][36][37][38][39][40]. H 2 O 2 is shown to be involved in the tolerance to various abiotic stresses acting as a secondary messenger [71], in cellular defense mechanisms against pathogens [72].…”

Section: The Roles Of Ros: Cell Signalingmentioning

confidence: 99%

“…ROS are also proved to act as a positive signal in seed dormancy release [30][31][32][33]. The dual function of ROS in plants depends on the levels of antioxidant compounds, and enzyme activities release [34][35][36][37][38]. By this way, plants can eliminate potentially harmful ROS that is produced under stress conditions, or control ROS concentrations in order to regulate various signaling pathways [34][35][36][37][38].…”

Section: Introductionmentioning

confidence: 99%

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Free Radicals and Antioxidant System in Seed Biology

Pehlivan¹

2017

Advances in Seed Biology

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Reactive oxygen species (ROS) are involved in various development stages of seed biology. During seed desiccation, germination and aging, oxidative stress may increase in higher levels, leading to cellular damage and seed deterioration. Plant cells have antioxidant system, detoxifying enzymes and antioxidant compounds, that scavenge ROS, participating in seed survival. This antioxidant system has various roles in desiccation and germination of developing seeds, seed storability, and seed aging. On the other hand, ROS are accepted as molecules involving in cellular signaling, and having regulatory functions in seed development. ROS are also found to have roles in gene expression in early embryogenesis, dormancy and germination. Abscisic acid is a plant hormone and a signaling molecule in seed development and that is reported to have relationships with ROS. The objective of this article is to review the roles of ROS and the importance of antioxidant system in orthodox seeds, and to emphasize the dual efects of ROS in seed biology.

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Section: The Roles Of Ros: Cell Signalingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Free Radicals and Antioxidant System in Seed Biology

Pehlivan¹

2017

Advances in Seed Biology

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“…The germination of the seeds increased progressively from 2 minutes (16.17%) pre-treatment period to 15 minutes (24%) and thereafter, decreased to 10.0% for 30 minutes duration. In contrast to other studies, H 2 O 2 was reported to increase germination of pea seeds (Gregorio et al, 2012); in cereals (Yushi et al, 2008); effectively improve germination of Zinnia elegans (Jacq) seeds (Dorota, 2014), and stimulated germination in seeds of Fagus orientalis (Afsaneh, Farshd, www.ccsenet.org/ijb International Journal of Biology Vol. 8, No.…”

Section: Pretreatment With Hydrogen Peroxide (H 2 O 2 )mentioning

confidence: 55%

“…The germination of the seeds of S. obtusifolia has continued to be problematic and the seeds may require different scarification methods and exogenous treatments with chemical to break dormancy and enhance germination, as have been reported for other species (Tambari & Aminu, 2015;Al-Menaie, 2010;Ramamoorthy, 2005;Rolston, 1978;Nalawadi, 1977;Asghar, Ali, & Mozhgan, 2014;Emongor, Mathowa, & Kabelo, 2004;Irfan et al, 2013;Ajiboye, 2010;Idu, Omonhinmin, & Onyibe, 2007;Salehi, 2008;Corbineau, Gouble, Lecat, & Come, 1991;Asghar et al, 2014;Mohammad , Faezeh, & Vajihe, 2014;Gregorio, José, & Pedro, 2012;Yushi, Kouhei, Tomoya, Takashi, & Mari, 2008). Studies on pre-treatment methods to break dormancy in S. obtusifolia seeds have not received desired attention.…”

Section: Introductionmentioning

confidence: 99%

Effects of Different Pretreatments and Seed Coat on Dormancy and Germination of Seeds of Senna obtusifolia (L.) H.S. Irwin & Barneby (Fabaceae)

Mensah¹,

Ekeke²

2016

IJB

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The seed dormancy of Senna obtusifolia was investigated through various methods, namely pretreatments in concentrated sulfuric acid, 2% potassium nitrate (KNO 3 ), 99% ethanol, 99% methanol, and in hydrogen perioxide; examination of the seed coverings; and the determination of water uptake by the seeds in order to ascertain the most effective technique for breaking dormancy and also determine the dormancy type. The results showed that sulfuric acid treatment recorded the highest germination (100%); followed by 2% hydrogen peroxide treatment (24%) in 15minutes immersion. The methanol and ethanol pretreatments gave 18.33% and 16.5% germinations respectively. Pretreatment in 2% potassium nitrate gave the lowest germination (8.50%), while the intact seeds of S. obtusifiolia (control) gave 0% germination. The anatomy of the seed coat indicated the presence of hard, thickened and specialized cells of cuticle, macrosclereids, osteoscereids, and disintegrated parenchyma layers. The water uptake of intact seeds was low (13.5%) after 24 hr imbibitions. These findings revealed that the seed coat acts as barrier to germination by preventing water absorption, possibly gaseous diffusion in and out of the seed and conferring mechanical resistance to the protrusion of embryo. Pretreatments, such as immersion in H 2 SO 4 will soften the seed coat and permit germination. Seed dormancy in S. obtusifolia can be considered of physical nature and classified as physical dormancy. The results obtained in this study may serve as useful information in the production and improvement of S. obtusifolia seedlings, as knowledge on seed dormancy and germination is a critical factor and requirements to the understanding of the propagation of this plant either in situ or ex-situ, in view of the economic potentials/attributes of this species.

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“…H 2 O 2 has been designated as a ''priming factor'' that coordinates the initiation of seed germination in pea by altering proteome, transcriptome and hormone levels. It has been postulated to induce a (Mitogen Activated Protein Kinase) MAPK-dependent decrease in abscisic acid level in the seed along with carbonylation of seed storage proteins (Barba-Espin et al 2012). The increase in the hydrogen peroxide levels in the initial stage of germination (30 h) in magnetically primed seeds could be explained by 66% increase in NADH peroxidase activity in the primed seeds (Fig.…”

Section: Free Radical Production and Scavenging By Antioxidant Systemmentioning

confidence: 98%

Pulsed magnetic field improves seed quality of aged green pea seeds by homeostasis of free radical content

et al. 2016

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To elucidate the mechanism responsible for magnetic field induced seed invigoration in aged seeds an experiment was conducted on six year old garden pea seeds stored under controlled (20°C and 40% RH) condition. Aged seeds were magnetoprimed by exposing to pulsed magnetic field (PMF) of 100 mT for 1 h in three pulsed modes. The 6 min on and off PMF showed significant improvement in germination (7.6%) and vigor (84.8%) over aged seeds. Superoxide and hydrogen peroxide production increased in germinating primed seeds by 27 and 52%, respectively, over aged seeds. Nicotinamide adenine dinucleotide (reduced) (NADH) peroxidase and superoxide dismutase involved in generation of hydrogen peroxide showed increased activity in PMF primed seeds. Increase in catalase, ascorbate peroxidase and glutathione reductase activity after 36 h of imbibition in primed seeds demonstrated its involvement in seed recovery during magnetopriming. An increase in total antioxidants also helped in maintaining the level of free radicals for promoting germination of magnetoprimed seeds. A 44% increase in level of protein carbonyls after 36 h indicated involvement of protein oxidation for counteracting and/or utilizing the production of ROS and faster mobilization of reserve proteins. Higher production of free radicals in primed seeds did not cause lipid peroxidation as malondialdehyde content was low. Lipoxygenase was involved in the germination associated events as the magnitude of activity was higher in primed aged seeds compared to aged seeds. Our study elucidated that PMF mediated improvement in seed quality of aged pea seeds was facilitated by fine tuning of free radicals by the antioxidant defense system and protein oxidation.

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Role of H₂O₂in pea seed germination

Cited by 83 publications

References 14 publications

Free Radicals and Antioxidant System in Seed Biology

Free Radicals and Antioxidant System in Seed Biology

Effects of Different Pretreatments and Seed Coat on Dormancy and Germination of Seeds of Senna obtusifolia (L.) H.S. Irwin & Barneby (Fabaceae)

Pulsed magnetic field improves seed quality of aged green pea seeds by homeostasis of free radical content

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Role of H2O2in pea seed germination

Cited by 83 publications

References 14 publications

Free Radicals and Antioxidant System in Seed Biology

Free Radicals and Antioxidant System in Seed Biology

Effects of Different Pretreatments and Seed Coat on Dormancy and Germination of Seeds of Senna obtusifolia (L.) H.S. Irwin & Barneby (Fabaceae)

Pulsed magnetic field improves seed quality of aged green pea seeds by homeostasis of free radical content

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Role of H₂O₂in pea seed germination