Role of habitats and sampling techniques on macroinvertebrate descriptors and ecological indicators: An experiment in a protected Mediterranean lagoon

Pinna, Maurizio; Janzen, Sally; Franco, Antonio; Specchia, Valeria; Marini, Gabriele

doi:10.1016/j.ecolind.2017.08.022

Cited by 16 publications

(11 citation statements)

References 48 publications

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“…260/10). In fact, benthic macroinvertebrates possess many of the hallmark traits of good bioindicators: poor mobility, high number of species and functional groups, long life cycles and their important role in aquatic trophic networks [2][3][4][5]. Moreover, any natural or anthropogenic perturbation causes a change in the structure and composition of their communities [42][43][44][45][46].…”

Section: Discussionmentioning

confidence: 99%

“…The DNA extraction process was completed with DNeasy PowerSoil kit (Qiagen) according to the manufacturer's protocol. For sampling macroinvertebrates, we used a Reineck box-corer covering a surface of 17 × 17 cm [2]. Afterwards, the samples collected were screened using a 1-mm square mesh sieve to eliminate interstitial water, fine sediments and debris.…”

Section: Single Species Dna-barcodingmentioning

confidence: 99%

“…Anthropogenic influences are causing unprecedented changes to the rate of biodiversity loss and, consequently, on ecosystem function [1]. The monitoring of biodiversity, as well as the analysis of occurrence and abundance of target taxonomic groups,is essential for evaluating the health of ecosystems and environmental change in marine coastal waters, transitional waters and freshwaters [2][3][4][5]. However, there is a lack of information regarding the biodiversity of the planet, due to monitoring difficulties.…”

Section: Introductionmentioning

confidence: 99%

“…There is a worldwide effort to enact legislation with the purpose of promoting ecological quality assessment of aquatic ecosystems [2]. In Europe, there are two main directives focusing on the conservation and protection of marine, transitional and coastal waters.…”

Section: Introductionmentioning

confidence: 99%

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Gap Analysis for DNA Barcode Reference Libraries for Aquatic Macroinvertebrate Species in the Apulia Region (Southeast of Italy)

Specchia

Tzafesta

Marini

et al. 2020

JMSE

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The use of molecular tools (DNA barcoding and metabarcoding) for the identification of species and ecosystem biomonitoring is a promising innovative approach. The effectiveness of these tools is, however, highly dependent on the reliability and coverage of the DNA sequence reference libraries and it also depends on the identification of primer sets that work on the broadest range of taxa. In this study, a gap analysis of available DNA barcodes in the international libraries was conducted using the aquatic macroinvertebrate species checklist of the Apulia region in the southeast of Italy. Our analyses show that 42% of the 1546 examined species do not have representative DNA barcodes in the reference libraries, indicating the importance of working toward their completeness and addressing this effort toward specific taxonomic groups. We also analyzed the DNA barcode reference libraries for the primer set used to barcode species. Only for 52% of the examined barcoded species were the primers reported, indicating the importance of uploading this information in the databases for a more effective DNA barcode implementation effort and extensive use of the metabarcoding method. In this paper, a new combination of primers has revealed its experimental effectiveness at least on the species belonging to the three most represented taxa in the aquatic ecosystems of the Apulia region, highlighting the opportunity to develop combinations of primers useful at the regional level and the importance of studying DNA barcode gaps at the local/regional level. The DNA barcode coverage also varies among different taxonomic groups and aquatic ecosystem types in which a large number of species are rare. We tested the application of the DNA barcoding single species to a lagoon ecosystem (the lagoon named “Acquatina di Frigole” in the Apulia region) and we sampled two macroinvertebrate species lacking DNA barcodes from “Aquatina di Frigole” NATURA 2000 Site IT9150003, Fabulina fabula and Tritia nitida, generated two new CO1 barcodes and added them to a DNA barcode reference library.

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Section: Discussionmentioning

confidence: 99%

Section: Single Species Dna-barcodingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Gap Analysis for DNA Barcode Reference Libraries for Aquatic Macroinvertebrate Species in the Apulia Region (Southeast of Italy)

Specchia

Tzafesta

Marini

et al. 2020

JMSE

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“…Since the 2000s, a globally-shared challenge is to maintain the supply of ecosystem services -commonly defined as the benefits to humans deriving from the ecosystems' dynamics -without impacting the biodiversity, processes and quality of an ecosystem (Birk et al 2012, Lin and Egerer 2020, Pinna et al 2016, Russo et al 2018. In order to protect ecosystems from damage and to restore degraded ones to a "good status", extensive regional, national, international and transnational regulations have been adopted.…”

Section: Introductionmentioning

confidence: 99%

Building a transnational biodiversity geo-database of the protected areas in the Adriatic-Ionian Macro-Region: approaches and results from the IMPRECO Project

Zangaro¹,

Marini²,

Specchia³

et al. 2021

BDJ

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The main objective of the project Common strategies and best practices to IMprove the transnational PRotection of ECOsystem integrity and services - IMPRECO is to enhance the safeguarding of ecosystems and ecosystem services. Additionally, the aim of this project is to tackle their environmental vulnerability by strengthening the potential of the Protected Areas in biodiversity, ecosystems and ecosystem services conservation. This is expected to be addressed by maintaining it through their transnational networking located in the European Adriatic-Ionian Macro-Region. The aim of this research is: 1) to characterise the habitats and ecosystems involved in the coastal-marine protected areas considered; 2) to set a biodiversity baseline; 3) to understand what current ecosystems’ conditions are; 4) to build up a transnational biomonitoring programme of target species and habitats and 5) to assess their response to pilot actions. To do so, a transnational inventory of species, habitats, ecosystems and ecosystem services was established, starting with the seven coastal-marine protected areas involved in the project. Data collection was carried out using different sources of information: scientific literature, officially available data from NATURA 2000 Standard Data Forms, checklists from local biomonitoring programmes, personal observations and citizen science, historical maps and data from new in-field analyses. Data were filled in the transnational biodiversity geo-databases according to the NATURA 2000 standards about habitat features, species protection level and species features. The presence of alien species (non-indigenous species, NIS) was also acknowledged and references about data collection were provided in the databases according to the Darwin Core standards.

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Reproductive biology of Ophelia barquii (Annelida, Opheliidae) along the Salento Peninsula (Mediterranean Sea, South Italy)

et al. 2020

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Role of habitats and sampling techniques on macroinvertebrate descriptors and ecological indicators: An experiment in a protected Mediterranean lagoon

Cited by 16 publications

References 48 publications

Gap Analysis for DNA Barcode Reference Libraries for Aquatic Macroinvertebrate Species in the Apulia Region (Southeast of Italy)

Gap Analysis for DNA Barcode Reference Libraries for Aquatic Macroinvertebrate Species in the Apulia Region (Southeast of Italy)

Building a transnational biodiversity geo-database of the protected areas in the Adriatic-Ionian Macro-Region: approaches and results from the IMPRECO Project

Reproductive biology of Ophelia barquii (Annelida, Opheliidae) along the Salento Peninsula (Mediterranean Sea, South Italy)

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