Role of Halotolerant Microbes in Plant Growth Promotion Under Salt Stress Conditions

Zahir, Zahir Ahmad; Nadeem, Sajid; Khan, Muhammad Yahya; Binyamin, Rana; Waqas, Muhammad

doi:10.1007/978-981-13-8335-9_10

Cited by 19 publications

(16 citation statements)

References 230 publications

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“…In addition, increased proline content protects membrane proteins and enzymes from oxidative burst (Szabados and Savouré, 2010). Several studies have confirmed the ability of microbes to mitigate the effects of oxidative bursts by increasing the activity of osmolyte contents and antioxidant enzymes (Khanna et al, 2019;Rajput et al, 2019;Vaishnav et al, 2019;Zahir et al, 2019). Interestingly, BHU-AV3-inoculated plant leaves exhibited less proline content compared to un-inoculated plants.…”

Section: Discussionmentioning

confidence: 98%

Sphingobacterium sp. BHU-AV3 Induces Salt Tolerance in Tomato by Enhancing Antioxidant Activities and Energy Metabolism

Vaishnav

Singh

et al. 2020

Front. Microbiol.

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Salt tolerant bacteria can be helpful in improving a plant's tolerance to salinity. Although plant-bacteria interactions in response to salt stress have been characterized, the precise molecular mechanisms by which bacterial inoculation alleviates salt stress in plants are still poorly explored. In the present study, we aimed to determine the role of a salt-tolerant plant growth-promoting rhizobacteria (PGPR) Sphingobacterium BHU-AV3 for improving salt tolerance in tomato through investigating the physiological responses of tomato roots and leaves under salinity stress. Tomato plants inoculated with BHU-AV3 and challenged with 200 mM NaCl exhibited less senescence, positively correlated with the maintenance of ion balance, lowered reactive oxygen species (ROS), and increased proline content compared to the non-inoculated plants. BHU-AV3-inoculated plant leaves were less affected by oxidative stress, as evident from a reduction in superoxide contents, cell death, and lipid peroxidation. The reduction in ROS level was associated with the increased antioxidant enzyme activities along with multiple-isoform expression [peroxidase (POD), polyphenol oxidase (PPO), and superoxide dismutase (SOD)] in plant roots. Additionally, BHU-AV3 inoculation induced the expression of proteins involved in (i) energy production [ATP synthase], (ii) carbohydrate metabolism (enolase), (iii) thiamine biosynthesis protein, (iv) translation protein (elongation factor 1 alpha), and the antioxidant defense system (catalase) in tomato roots. These findings have provided insight into the molecular mechanisms of bacteria-mediated alleviation of salt stress in plants. From the study, we can conclude that BHU-AV3 inoculation effectively induces antioxidant systems and energy metabolism in tomato roots, which leads to whole plant protection during salt stress through induced systemic tolerance.

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Section: Discussionmentioning

confidence: 98%

Sphingobacterium sp. BHU-AV3 Induces Salt Tolerance in Tomato by Enhancing Antioxidant Activities and Energy Metabolism

Vaishnav

Singh

et al. 2020

Front. Microbiol.

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“…The IST mechanisms induced by PGPB includes [1, 17,18]: solubilization of nutrients; activation of stress-responsive genes; regulation of phytohormone synthesis (e.g., 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity); promotion of antioxidant defenses; regulation of osmotic balance (e.g., proline accumulation), turgor pressure, ion homeostasis, and production of volatile organic compounds. Moreover, halophilic/halotolerant PGPB can survive in the presence of high salt concentrations [19]. Halophily/halotolerance and IST mechanisms induced by PGPB allow plants to survive under high salt concentrations [2,[19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, halophilic/halotolerant PGPB can survive in the presence of high salt concentrations [19]. Halophily/halotolerance and IST mechanisms induced by PGPB allow plants to survive under high salt concentrations [2,[19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Plant Growth-Promoting Traits and Inoculation Effects on Triticum durum of Actinomycetes Isolates under Salt Stress Conditions

et al. 2021

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This study aimed to characterize the halotolerant capability, in vitro, of selected actinomycetes strains and to evaluate their competence in promoting halo stress tolerance in durum wheat in a greenhouse experiment. Fourteen isolates were tested for phosphate solubilization, indole acetic acid, hydrocyanic acid, and ammonia production under different salt concentrations (i.e., 0, 0.25, 0.5, 0.75, 1, 1.25, and 1.5 M NaCl). The presence of 1-aminocyclopropane-1-carboxylate deaminase activity was also investigated. Salinity tolerance was evaluated in durum wheat through plant growth and development parameters: shoot and root length, dry and ash-free dry weight, and the total chlorophyll content, as well as proline accumulation. In vitro assays have shown that the strains can solubilize inorganic phosphate and produce indole acetic acid, hydrocyanic acid, and ammonia under different salt concentrations. Most of the strains (86%) had 1-aminocyclopropane-1-carboxylate deaminase activity, with significant amounts of α-ketobutyric acid. In the greenhouse experiment, inoculation with actinomycetes strains improved the morpho-biochemical parameters of durum wheat plants, which also recorded significantly higher content of chlorophylls and proline than those uninoculated, both under normal and stressed conditions. Our results suggest that inoculation of halotolerant actinomycetes can mitigate the negative effects of salt stress and allow normal growth and development of durum wheat plants.

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“…ROS plays a significant role as signaling molecules at low concentration, but at higher level they cause oxidative damage and affect membranes, lipids, nucleic acids and proteins ( Del Rio, 2015 ). Several reports have confirmed that the ability of plant cell and microbes to mitigate the effects of oxidative burst occurs by improving the accumulation of osmolytes and antioxidant enzymes ( Egamberdieva et al, 2017 ; Singh and Jha, 2017 ; Saikia et al, 2018 ; Khan and Bano, 2019 ; Khanna et al, 2019 ; Rajput et al, 2019 ; Vaishnav et al, 2019 ; Zahir et al, 2019 ; Nagpal et al, 2020 ). Our study illustrated that dual inoculants of LSMR-32 + LSMRS-3 reduced osmotic and oxidative stress in spring mungbean plants by inducing accumulation of proline and antioxidant enzymes (CAT, POX and SOD) in roots, exposed to salt stress.…”

Section: Discussionmentioning

confidence: 95%

Dual Microbial Inoculation, a Game Changer? – Bacterial Biostimulants With Multifunctional Growth Promoting Traits to Mitigate Salinity Stress in Spring Mungbean

et al. 2021

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Soil microbes play a vital role in improving plant growth, soil health, ameliorate biotic/abiotic stress and enhance crop productivity. The present study was aimed to investigate a coordinated effect of compatible consortium [salt tolerating Rhizobium and rhizobacterium with 1-aminocyclopropane-1-carboxylate (ACC) deaminase] in enhancing plant growth promoting (PGP) traits, symbiotic efficiency, nutrient acquisition, anti-oxidative enzymes, grain yield and associated profitability in spring mungbean. We identified a non-pathogenic compatible Rhizobium sp. LSMR-32 (MH644039.1) and Enterococcus mundtii LSMRS-3 (MH644178.1) from salt affected areas of Punjab, India and the same were assessed to develop consortium biofertilizer based on salt tolerance, multifarious PGP traits, antagonistic defense activities and presence of nifH, acds, pqq, and ipdc genes. Indole Acetic acid (IAA), P-solubilization, biofilm formation, exo-polysaccharides, siderophore, salt tolerance, ACC deaminase activities were all found highly significant in dual inoculant (LSMR-32 + LSMRS-3) treatment compared to LSMR-32 alone. Under saline soil conditions, dual inoculant showed a higher seed germination, plant height, biomass, chlorophyll content and macro and micro-nutrient uptake, than un-inoculated control. However, symbiotic (nodulation, nodule biomass and leghaemoglobin content) and soil quality parameters (phosphatase and soil dehydrogenase enzymes) increased numerically with LSMR-32 + LSMRS-3 over Rhizobium sp. LSMR-32 alone. Dual bacterial inoculation (LSMR-32 + LSMRS-3) increased the proline content (2.05 fold), anti-oxidative enzymes viz., superoxide dismutase (1.50 fold), catalase (1.43 fold) and peroxidase (3.88 folds) in contrast to control treatment. Decreased Na+ accumulation and increased K+ uptake resulted in favorable K+/Na+ ratio through ion homeostasis. Co-inoculation of Rhizobium sp. LSMR-32 and Enterococcus mundtii LSMRS-3 significantly improved the grain yield by 8.92% and led to superior B: C ratio over Rhizobium sp. alone under salt stress. To best of our knowledge this is perhaps the first field report from Indian soils that largely describes dual inoculation of Rhizobium sp. LSMR-32 and Enterococcus mundtii LSMRS-3 and the same can be considered as a game-changer approach to simultaneously induce salt tolerance and improve productivity in spring mungbean under saline stress conditions.

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Role of Halotolerant Microbes in Plant Growth Promotion Under Salt Stress Conditions

Cited by 19 publications

References 230 publications

Sphingobacterium sp. BHU-AV3 Induces Salt Tolerance in Tomato by Enhancing Antioxidant Activities and Energy Metabolism

Sphingobacterium sp. BHU-AV3 Induces Salt Tolerance in Tomato by Enhancing Antioxidant Activities and Energy Metabolism

Characterization of Plant Growth-Promoting Traits and Inoculation Effects on Triticum durum of Actinomycetes Isolates under Salt Stress Conditions

Dual Microbial Inoculation, a Game Changer? – Bacterial Biostimulants With Multifunctional Growth Promoting Traits to Mitigate Salinity Stress in Spring Mungbean

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