Nowadays, operational costs of culturing Spirulina, notably the costs of the culture media and the regulation of inhibitors in the medium, are the main barriers for scaling up this bioprocess. Hence, the present study was aimed to investigate the effect of different vermicomposts incorporated with Zarrouk Medium (ZM) for the cultivation of Spirulina. In this study, experiments consists of 8 groups. Group 1 is vermimedia (VM) prepared from vermicompost using Eudrilus eugeniae with ZM (10 ml/l); group 2 is VM prepared from decomposed materials of Jasminum sambac using E. eugeniae worms with ZM (10 ml/l); group 3 is VM prepared from decomposed materials of Celosia argentea using E. eugeniae worms with ZM (10 ml/l); group 4 is VM prepared from decomposed materials of Penaeus indicus compost using E. eugeniae worms with ZM (10 ml/l); group 5 is VM prepared from vermicompost using Eisenia fetida with ZM (10 ml/l); group 6 is VM prepared from decomposed materials of J. sambac using E. fetida worms with ZM (10 ml/l); group 7 is VM prepared from decomposed materials of C. argentea using E. fetida worms with ZM (10 ml/l); and group 8 is VM prepared from decomposed materials of P. indicus compost using E. fetida worms with ZM (10 ml/l). The total biomass, chlorophyll and major biochemical profile contents were estimated using standard methods. For this study, the P. indicus compost using E. eugeniae worms with ZM has resulted in highest biomass concentration when compared to other VM materials (2.2±0.3 g/l) and the highest protein and chlorophyll content was also recorded in group 3. The biochemical profiles such as nitrogen (7.3±0.32), calcium (316.60±36.97), chlorophyll (1408.987±7.505), potassium (796.2533±6.35), magnesium (796.2533±6.35), iron (20.63±1.7) and total organic carbon (28.12±3.46) were also significantly higher in the group 3 when compared to other VM. Based on our experimental results, it is concluded that modified organic media could be used for better biomass yield.