Role of hemocytin from Litopenaeus vannamei in immune response against microsporidian, Enterocytozoon hepatopenaei

Sukonthamarn, Pongsakorn; Nanakorn, Zittipong; Junprung, Wisarut; Supungul, Premruethai; Tassanakajon, Anchalee

doi:10.1016/j.fsi.2023.108710

Cited by 3 publications

(3 citation statements)

References 36 publications

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“…Therefore, we next focused on two marker genes from the Immune 1 hemocyte cluster: hemocytin and astakine (Figure 4A, Supplementary . Hemocytin is homologous to hemolectin in D. melanogaster and von Willebrand factors of mammals (24)(25)(26). Hemocytin encodes for a large multidomain adhesive protein involved in clotting, microbial agglutination and hemocyte aggregation (24)(25)(26).…”

Section: Hemocytin and Astakine Contribute To A Phagocytophilum Infec...mentioning

confidence: 99%

“…Hemocytin is homologous to hemolectin in D. melanogaster and von Willebrand factors of mammals (24)(25)(26). Hemocytin encodes for a large multidomain adhesive protein involved in clotting, microbial agglutination and hemocyte aggregation (24)(25)(26). Conversely, astakine is a cytokine-like molecule present in chelicerates and crustaceans and is homologous to vertebrate prokineticins (27)(28)(29)(30).…”

Section: Hemocytin and Astakine Contribute To A Phagocytophilum Infec...mentioning

confidence: 99%

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Tick hemocytes have pleiotropic roles in microbial infection and arthropod fitness

Rolandelli,

Laukaitis-Yousey,

Bogale

et al. 2023

Preprint

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Uncovering the complexity of systems in non-model organisms is critical for understanding arthropod immunology. Prior efforts have mostly focused on Dipteran insects, which only account for a subset of existing arthropod species in nature. Here, we describe immune cells or hemocytes from the clinically relevant tickIxodes scapularisusing bulk and single cell RNA sequencing combined with depletion via clodronate liposomes, RNA interference, Clustered Regularly Interspaced Short Palindromic Repeats activation (CRISPRa) and RNA-fluorescencein situhybridization (FISH). We observe molecular alterations in hemocytes upon tick infestation of mammals and infection with either the Lyme disease spirocheteBorrelia burgdorferior the rickettsial agentAnaplasma phagocytophilum. We predict distinct hemocyte lineages and reveal clusters exhibiting defined signatures for immunity, metabolism, and proliferation during hematophagy. Furthermore, we perform a mechanistic characterization of twoI. scapularishemocyte markers:hemocytinandastakine. Depletion of phagocytic hemocytes affectshemocytinandastakinelevels, which impacts blood feeding and molting behavior of ticks. Hemocytin specifically affects the c-Jun N-terminal kinase (JNK) signaling pathway, whereas astakine alters hemocyte proliferation inI. scapularis. Altogether, we uncover the heterogeneity and pleiotropic roles of hemocytes in ticks and provide a valuable resource for comparative biology in arthropods.

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Section: Hemocytin and Astakine Contribute To A Phagocytophilum Infec...mentioning

confidence: 99%

Section: Hemocytin and Astakine Contribute To A Phagocytophilum Infec...mentioning

confidence: 99%

Tick hemocytes have pleiotropic roles in microbial infection and arthropod fitness

Rolandelli,

Laukaitis-Yousey,

Bogale

et al. 2023

Preprint

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show abstract

“…In silkworm Bombyx mori , the Toll, IMD, and JAK/STAT signaling pathways are induced by microsporidian Nosema bombycis infection, which leads to the production of antimicrobial peptides including lebocin, gloverin, cecropin, and attacin families [ 32 , 33 ]. Similar to silkworm, these signaling pathways in shrimp are important in EHP clearance by increasing proPO activating cascade and several immune-related genes to combat EHP proliferation [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Immune signaling of Litopenaeus vannamei c-type lysozyme and its role during microsporidian Enterocytozoon hepatopenaei (EHP) infection

Sangklai,

Supungul,

Jaroenlak

et al. 2024

PLoS Pathog

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The microsporidian Enterocytozoon hepatopenaei (EHP) is a fungi-related, spore-forming parasite. EHP infection causes growth retardation and size variation in shrimp, resulting in severe economic losses. Studies on shrimp immune response have shown that several antimicrobial peptides (AMPs) were upregulated upon EHP infection. Among those highly upregulated AMPs is c-type lysozyme (LvLyz-c). However, the immune signaling pathway responsible for LvLyz-c production in shrimp as well as its function against the EHP infection are still poorly understood. Here, we characterized major shrimp immune signaling pathways and found that Toll and JAK/STAT pathways were up-regulated upon EHP infection. Knocking down of a Domeless (DOME) receptor in the JAK/STAT pathways resulted in a significant reduction of the LvLyz-c and the elevation of EHP copy number. We further elucidated the function of LvLyz-c by heterologously expressing a recombinant LvLyz-c (rLvLyz-c) in an Escherichia coli. rLvLyz-c exhibited antibacterial activity against several bacteria such as Bacillus subtilis and Vibrio parahaemolyticus. Interestingly, we found an antifungal activity of rLvLyz-c against Candida albican, which led us to further investigate the effects of rLvLyz-c on EHP spores. Incubation of the EHP spores with rLvLyz-c followed by a chitin staining showed that the signals were dramatically decreased in a dose-dependent manner, suggesting that rLvLyz-c possibly digest a chitin coat on the EHP spores. Transmission electron microscopy analysis revealed that an endospore layer, which is composed mainly of chitin, was digested by rLvLyz-c. Lastly, we observed that EHP spores that were treated with rLvLyz-c showed a significant reduction of the spore germination rate. We hypothesize that thinning of the endospore of EHP would result in altered permeability, hence affecting spore germination. This work provides insights into shrimp immune signaling pathways responsible for LvLyz-c production and its anti-EHP property. This knowledge will serve as important foundations for developing EHP control strategies.

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Revelation of benzo(a)pyrene-induced latent toxicity mechanism to intestinal histomorphology and oxidative status for common carp (Cyprinus carpio) through transcriptional and single-cell sequencing

Hao,

Chen,

Fan

et al. 2024

Aquaculture

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Role of hemocytin from Litopenaeus vannamei in immune response against microsporidian, Enterocytozoon hepatopenaei

Cited by 3 publications

References 36 publications

Tick hemocytes have pleiotropic roles in microbial infection and arthropod fitness

Tick hemocytes have pleiotropic roles in microbial infection and arthropod fitness

Immune signaling of Litopenaeus vannamei c-type lysozyme and its role during microsporidian Enterocytozoon hepatopenaei (EHP) infection

Revelation of benzo(a)pyrene-induced latent toxicity mechanism to intestinal histomorphology and oxidative status for common carp (Cyprinus carpio) through transcriptional and single-cell sequencing

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