Role of Herpes Virus in Periodontal Diseases

Varghese, Sheenu

doi:10.9790/0853-13117173

Cited by 2 publications

(2 citation statements)

References 34 publications

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“…Based on the hypothesis that the presence of herpes viruses would be significantly higher among patients with periodontitis, our study showed that its presence was high in aggressive periodontitis (55%), although this was not statistically significant (Fisher's exact test, P = 0.147). This seems to have been the conclusion for several other studies, which found that herpes viruses target host cells by attaching onto the cell surface through glycoproteins present in the viral envelope, stimulating local cytokine production through macrophages and other inflammatory cells. This phenomenon coincides with the state of elevated localized inflammatory burden due to increased gingival crevicular fluid cytokine levels in aggressive periodontitis, which leads to the impairment of local periodontal immune defense .…”

Section: Discussionsupporting

confidence: 54%

Detection of herpes viruses in Ghanaian patients with periodontitis

Blankson

Obeng‐Nkrumah

et al. 2019

J of Invest & Clin Dent

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Aim The complexity of periodontitis in both etiology and progression has raised many questions, necessitating enormous research in recent years. The aim of the present study was to detect the presence of herpes viruses in Ghanaian patients diagnosed with periodontitis. Methods Thirty‐one patients were included in the study; 21 with periodontitis classified into localized chronic, generalized, and aggressive periodontitis, and 10 without the disease were used as controls. Subgingival samples were collected, followed by DNA extraction. Multiplex polymerase chain reaction was used to amplify viral DNA for the detection of herpes viruses. Data was analyzed using Stata 14. Results The mean age for patients with aggressive periodontitis was 32.2 years (standard deviation [SD]: 8.50), while those for localized chronic periodontitis and generalized chronic periodontitis were 40.6 years (SD: 7.83) and 46.3 years (SD: 12.12), respectively. Viruses were detected only among patients clinically diagnosed with aggressive periodontitis. Of the total number of aggressive periodontitis patients, herpes simplex virus 1 (HSV‐1) and Epstein‐Barr virus (HBV) were found in four (44%) and one (11%), respectively. The mean age for patients found to have HSV‐1 or EBV was 29 years (SD: 6.93). Conclusion We found HSV‐1 and EBV in the subgingival plaque samples of Ghanaian patients clinically diagnosed with aggressive periodontitis. While our finding requires further investigation, the role of HSV in periodontitis, if elucidated, could transform and inform the clinical management of the condition.

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Section: Discussionsupporting

confidence: 54%

Detection of herpes viruses in Ghanaian patients with periodontitis

Blankson

Obeng‐Nkrumah

et al. 2019

J of Invest & Clin Dent

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“…Another PCR technique, the nested PCR, was implemented to increase the specificity of EBV DNA amplification and reduce the non-specific amplification by the involvement of two sets of primers (outer and inner pairs) for the same target [ 37 , 38 , 39 , 40 , 41 ]. Additionally, the nested PCR is more efficient in detecting low viral loads [ 42 ]. Typically, the amplicons generated via these PCR methods are subsequently size-fractioned and detected by agarose (AGE) or polyacrylamide gel electrophoresis (PAGE) ( Table 1 ), or else, restriction fragment length polymorphism analysis (RFLP) with endonucleases followed by AGE can be applied.…”

Section: Polymerase Chain Reaction (Pcr)-based Detection Methodsmentioning

confidence: 99%

Detection of Epstein–Barr Virus in Periodontitis: A Review of Methodological Approaches

et al. 2020

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Periodontitis, an inflammatory condition that affects the structures surrounding the tooth eventually leading to tooth loss, is one of the two biggest threats to oral health. Beyond oral health, it is associated with systemic diseases and even with cancer risk. Obviously, periodontitis represents a major global health problem with significant social and economic impact. Recently, a new paradigm was proposed in the etiopathogenesis of periodontitis involving a herpesviral–bacterial combination to promote long-term chronic inflammatory disease. Periodontitis as a risk factor for other systemic diseases can also be better explained based on viral–bacterial etiology. Significant efforts have brought numerous advances in revealing the links between periodontitis and Epstein–Barr virus (EBV), a gamma herpesvirus ubiquitous in the adult human population. The strong evidence from these studies may contribute to the advancement of periodontitis research and the ultimate control of the disease. Advancing the periodontitis research will require implementing suitable methods to establish EBV involvement in periodontitis. This review evaluates and summarizes the existing methods that allow the detection and diagnosis of EBV in periodontitis (also applicable in a more general way to other EBV-related diseases), and discusses the feasibility of the application of innovative emerging technologies.

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Role of Herpes Virus in Periodontal Diseases

Cited by 2 publications

References 34 publications

Detection of herpes viruses in Ghanaian patients with periodontitis

Detection of herpes viruses in Ghanaian patients with periodontitis

Detection of Epstein–Barr Virus in Periodontitis: A Review of Methodological Approaches

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