Role of hesperetin (a natural flavonoid) and its analogue on apoptosis in HT-29 human colon adenocarcinoma cell line – A comparative study

Gunasekaran, S.; Rajamanickam, Vinothkumar; Preethy, Christo Paul; Riyasdeen, Anvarbatcha; Akbarsha, Mohammad Abdulkader; Menon, Venugopal Padmanaban; Nalini, Namasivayam

doi:10.1016/j.fct.2011.11.038

Cited by 69 publications

(34 citation statements)

References 35 publications

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“…AO/EB are vital dyes that distinguish live, apoptotic and dead cells. AO could stain the nuclei of viable and apoptotic cells, however, EB stains only cells that have lost membrane integrity (Sivagami et al, 2012). The results of staining showed in our study live cells appeared uniformly green and apoptotic cells stained yellow-green with bright dot in the nuclei as a consequence of chromatin condensation ( Figure 4A).…”

Section: Discussionmentioning

confidence: 55%

Luteolin Induced-growth Inhibition and Apoptosis of Human Esophageal Squamous Carcinoma Cell Line Eca109 Cells in vitro

Wang¹,

Wang²,

Huang³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Luteolin is a plant flavonoid which exhibits anti-oxidative, anti-inflammatory and anti-tumor effects. However, the antiproliferative potential of luteolin is not fully understood. In this study, we investigated the effect of luteolin on cell cycling and apoptosis in human esophageal squamous carcinoma cell line Eca109 cells. MTT assays showed that luteolin had obvious cytotoxicity on Eca109 with an IC 50 of 70.7±1.72μM at 24h. Luteolin arrested cell cycle progression in the G0/G1 phase and prevented entry into S phase in a dose-and time-dependent manner. as assessed by FCM. Luteolin induced apoptosis of Eca109 cells was demonstrated by AO/EB staining assay and annexin V-FITC/PI staining. Moreover, luteolin downregulated the expression of cyclin D1, survivin and c-myc, and it also upregulated the expression of p53, in line with the fact that luteolin was able to inhibit Eca109 cell proliferation.

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Section: Discussionmentioning

confidence: 55%

Luteolin Induced-growth Inhibition and Apoptosis of Human Esophageal Squamous Carcinoma Cell Line Eca109 Cells in vitro

Wang¹,

Wang²,

Huang³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…The ability to induce tumor cell apoptosis is an important property of a candidate anticancer drug, which discriminates between anticancer drugs and toxic compounds. Much effort has been directed towards searching for compounds that influence apoptosis and understanding their mechanism of action 22 .…”

Section: Discussionmentioning

confidence: 99%

EFFECT OF PLGAâ€“PEG NANOENCAPSULATION OF ACTIVE CONSTITUENTS FROM CARALLUMA ADSCENDENS (ROXB.) ON HT-29 COLON CANCER CELL

Gowri¹,

Taj²

2016

Int. J. Res. Ayurveda Pharm.

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Colon cancer is the most commonly diagnosed cancer disease in westernized countries its management remains a challenge in everyday oncology practice. Thus, advanced therapeutic strategies are required to treat colon cancer patients. Caralluma adscendens (Roxb.) (CAR) is a promising anticancer agent for various cancer types. The main objective of this study was to develop a polymeric drug delivery system for active components from Caralluma adscendens (Roxb.) intended to be intravenously administered, capable of improving the therapeutic index of the extract. To achieve this goal Caralluma adscendens (Roxb loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles (PEG-PLGA-Nps) were prepared by singleemulsion solvent-evaporation technique. The nanoparticles were characterized by SEM and TEM analysis. The in vitro cytotoxic activity of CAR-PLGA-Nps developed was assessed using a human colon cancer cell line (HT-29) in MTT assay and DNA fragmentation assay and compared to the in vitro anti-tumoral activity of the Caralluma adscendens (Roxb.) extract.

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“…Fisetin and hesperetin, plant-derived flavonoids, have been shown to produce a wide range of pharmacological effects including antioxidant, anti-inflammatory, and anticancer effects and found to induce apoptosis in various tumor cells by causing alterations in various cellular processes [7,13]. The potential anticarcinogenic effects of fisetin and hesperetin, naturally occurring flavonoids, have been considered in various cancer types especially solid tumors including lung, colon, and breast cancers through several different mechanisms [11,12,16,17]. However, to our knowledge, there is no detailed study investigating their mechanisms of action in APL.…”

Section: Discussionmentioning

confidence: 99%

“…Promising results derived from a noticeable body of evidence displayed that hesperetin could mediate its anticancer effects in several cancer types by affecting several cellular targets. Some of these mechanisms include modulation of apoptotic proteins [15], increased activity of several caspases like caspase-3 and caspase-9 [16], and arrest of cell cycle progression [17]. On the other hand, the mechanism(s) responsible for the biological effects of hesperetin in APL cells remain unidentified yet.…”

Section: Introductionmentioning

confidence: 99%

The pleiotropic effects of fisetin and hesperetin on human acute promyelocytic leukemia cells are mediated through apoptosis, cell cycle arrest, and alterations in signaling networks

Adan

Baran

2015

Tumor Biol.

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Fisetin and hesperetin, flavonoids from various plants, have several pharmaceutical activities including antioxidative, anti-inflammatory, and anticancer effects. However, studies elucidating the role and the mechanism(s) of action of fisetin and hesperetin in acute promyelocytic leukemia are absent. In this study, we investigated the mechanism of the antiproliferative and apoptotic actions exerted by fisetin and hesperetin on human HL60 acute promyelocytic leukemia cells. The viability of HL60 cells was evaluated using the MTT assay, apoptosis by annexin V/propidium iodide (PI) staining and cell cycle distribution using flow cytometry, and changes in caspase-3 enzyme activity and mitochondrial transmembrane potential. Moreover, we performed wholegenome microarray gene expression analysis to reveal genes affected by fisetin and hesperetin that can be important for developing of future targeted therapy. Based on data obtained from microarray analysis, we also described biological networks modulated after fisetin and hesperetin treatment by KEGG and IPA analysis. Fisetin and hesperetin treatment showed a concentration-and time-dependent inhibition of proliferation and induced G2/M arrest for both agents and G0/G1 arrest for hesperetin at only the highest concentrations. There was a disruption of mitochondrial membrane potential together with increased caspase-3 activity. Furthermore, fisetin-and hesperetin-triggered apoptosis was confirmed by annexin V/PI analysis. The microarray gene profiling analysis revealed some important biological pathways including mitogen-activated protein kinases (MAPK) and inhibitor of DNA binding (ID) signaling pathways altered by fisetin and hesperetin treatment as well as gave a list of genes modulated ≥2-fold involved in cell proliferation, cell division, and apoptosis. Altogether, data suggested that fisetin and hesperetin have anticancer properties and deserve further investigation.

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Role of hesperetin (a natural flavonoid) and its analogue on apoptosis in HT-29 human colon adenocarcinoma cell line – A comparative study

Cited by 69 publications

References 35 publications

Luteolin Induced-growth Inhibition and Apoptosis of Human Esophageal Squamous Carcinoma Cell Line Eca109 Cells in vitro

Luteolin Induced-growth Inhibition and Apoptosis of Human Esophageal Squamous Carcinoma Cell Line Eca109 Cells in vitro

EFFECT OF PLGAâ€“PEG NANOENCAPSULATION OF ACTIVE CONSTITUENTS FROM CARALLUMA ADSCENDENS (ROXB.) ON HT-29 COLON CANCER CELL

The pleiotropic effects of fisetin and hesperetin on human acute promyelocytic leukemia cells are mediated through apoptosis, cell cycle arrest, and alterations in signaling networks

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