Role of histamine in the regulation of intestinal immunity in fish

Galindo-Villegas, Jorge; García‐García, Erick; Mulero, Víctoriano

doi:10.1016/j.dci.2016.02.013

Cited by 33 publications

(20 citation statements)

References 106 publications

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“…Records on contents of granules of fish MCs revealed that they have a panel of inflammatory mediators (Da Silva et al., ; Dezfuli, Lui, Giovinazzo, Boldrini, & Giari, ; Galindo‐Villegas et al., ; Silphaduang & Noga, ). MCs degranulation was observed after inoculation of Aeromonas salmonicida exotoxins (Ellis, ; Vallejo & Ellis, ), although degranulation of MCs was induced ex vivo by use of compound 48/80 on intestinal tissue of rainbow trout (Manera, Giammarino, Borreca, Giari, & Dezfuli, ).…”

Section: Discussionmentioning

confidence: 99%

“…Such barriers are comprised of mucous cells, epithelial cells, neuroendocrine cells and different types of immune cells (Dezfuli, Manera, Giari, DePasquale, & Bosi, ; Dezfuli et al., , ; Jutfelt, ). Among immune cells, mast cells (MCs) (Buchmann, ; Dezfuli, Bo, Lorenzoni, Shinn, & Giari, ; Dezfuli et al., , ; Galindo‐Villegas, Garcia‐Garcia, & Mulero, ; Secombes & Ellis, ; Silphaduang & Noga, ), neutrophils (Havixbeck, Riger, Wong, Hodgkinson, & Barreda, ; Von Gersdorff Jørgensen, ), macrophages (Agius & Roberts, ; Hodgkinson, Grayfer, & Belosevic, ) and rodlet cells (RCs) (Bosi, Shinn, Giari, & Dezfuli, ; Dezfuli et al., ; Dezfuli, Manera et al., ; Manera & Dezfuli, ; Reite, ; Reite & Evensen, ) are active in immune response against parasites.…”

Section: Introductionmentioning

confidence: 99%

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Histopathological and ultrastructural assessment of two mugilid species infected with myxozoans and helminths

Dezfuli

Castaldelli

Giari

2017

Journal of Fish Diseases

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The histopathology and ultrastructure of the intestine of mullets, Liza ramada and Liza saliens, from Comacchio lagoons (northern Italy) naturally infected with myxozoans and helminths were investigated and described. Sixty-two (80.5%) of 77 mullets harboured one or more of the following parasites species: Myxobolus mugchelo (Myxozoa), Neoechinorhynchus agilis (Acanthocephala), Haplosplanchnus pachysomus and Dicrogaster contractus (Digenea). Co-occurrence of helminths with myxozoans was common. The main damage caused by digeneans was destruction of the mucosal epithelium of the villi, necrosis and degeneration of intestinal epithelial cells. More severe intestinal damage was caused by acanthocephalans which reach the submucosa layer with their proboscis. At the site of helminths infection, several mast cells (MCs), rodlet cells (RCs), mucous cells and few neutrophils and macrophages were observed in the epithelium. RCs and mucous cells exhibited discharge activity in close vicinity to the worm's tegument. M. mugchelo conspicuous plasmodia were encysted mainly in muscle and submucosa layers of the intestine. Indeed, spores of M. mugchelo were documented within the epithelial cells of host intestine and in proximity to MCs. Degranulation of the MCs near the myxozoans was very frequent.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Histopathological and ultrastructural assessment of two mugilid species infected with myxozoans and helminths

Dezfuli

Castaldelli

Giari

2017

Journal of Fish Diseases

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“…MCs are motile [25, 48] and are often strategically positioned at perivascular sites to regulate inflammation, to encounter invading organisms and to orchestrate a response [27, 42, 48–51]. MCs degranulate in response to a variety of pathogens [50, 52] and known degranulating agents [42, 53]. Degranulation of fish MCs close to the tegument of helminths in intestine and other organs was reported in Dezfuli et al [50, 54].…”

Section: Discussionmentioning

confidence: 99%

Nematode infection in liver of the fish Gymnotus inaequilabiatus (Gymnotiformes: Gymnotidae) from the Pantanal Region in Brazil: pathobiology and inflammatory response

et al. 2016

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BackgroundA survey on endoparasitic helminths from freshwater fishes in the Pantanal Region (Mato Grosso do Sul, Brazil) revealed the occurrence of third-larval stage of the nematode Brevimulticaecum sp. (Heterocheilidae) in most organs of Gymnotus inaequilabiatus (Gymnotidae) also known by the local name tuvira. The aim of the present study was to examine Brevimulticaecum sp.-infected tuvira liver at the ultrastructural level and clarify the nature of granulomas and the cellular elements involved in the immune response to nematode larvae.MethodsThirty-eight adult specimens of tuvira from Porto Morrinho, were acquired in January and March 2016. Infected and uninfected liver tissues were fixed and prepared for histological and ultrastructure investigations.ResultsThe prevalence of infection of tuvira liver by the nematode larvae was 95 %, with an intensity of infection ranging from 4 to 343 larvae (mean ± SD: 55.31 ± 73.94 larvae per liver). In livers with high numbers of nematode larvae, almost entire hepatic tissue was occupied by the parasites. Hepatocytes showed slight to mild degenerative changes and accumulation of pigments. Parasite larvae were surrounded by round to oval granulomas, the result of focal host tissue response to the infection. Each granuloma was typically formed by three concentric layers: an outer layer of fibrous connective tissue with thin elongated fibroblasts; a middle layer of mast cells entrapped in a thin fibroblast-connective mesh; and an inner layer of densely packed epithelioid cells, displaying numerous desmosomes between each other. Numerous macrophage aggregates occurred in the granulomas and in the parenchyma.ConclusionsOur results in tuvira showed that the larvae were efficiently sequestered within the granulomas, most of the inflammatory components were confined within the thickness of the granuloma, and the parenchyma was relatively free of immune cells and without fibrosis. Presumably this focal encapsulation of the parasites permits uninfected portions of liver to maintain its functions and allows the survival of the host.

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“…In the human intestine, several functions such as fluid and ion transport are influenced by histamine [ 56 ]. Nonetheless, the most studied aspect of histamine function is its involvement in inflammation [ 57 – 59 ]. It seems that one of the most controversial aspects of the biology of MCs of early vertebrates is the presence of histamine in their granules [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Pike intestinal reaction to Acanthocephalus lucii (Acanthocephala): immunohistochemical and ultrastructural surveys

et al. 2018

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BackgroundThe Northern pike, Esox lucius, is a large, long-lived, top-predator fish species and occupies a broad range of aquatic environments. This species is on its way to becoming an important model organism and has the potential to contribute new knowledge and a better understanding of ecology and evolutionary biology. Very few studies have been done on the intestinal pathology of pike infected with helminths. The present study details the first Italian record of adult Acanthocephalus lucii reported in the intestine of E. lucius.ResultsA total of 22 pike from Lake Piediluco (Central Italy) were examined, of which 16 (72.7%) were infected with A. lucii. The most affected areas of gastrointestinal tract were the medium and distal intestine. The intensity of infection ranged from 1 to 18 parasites per host. Acanthocephalus lucii penetrated mucosal and submucosal layers which had a high number of mast cells (MCs) with an intense degranulation. The cellular elements involved in the immune response within the intestine of pike were assessed by ultrastructural techniques and immunohistochemistry using antibodies against met-enkephalin, immunoglobulin E (IgE)-like receptor (FCεRIγ), histamine, interleukin-6, interleukin-1β, substance P, lysozyme, serotonin, inducible-nitric oxide synthase (i-NOS), tumor necrosis factor-α (TNF-α) and the antimicrobial peptide piscidin 3 (P3). In intestines of the pike, several MCs were immunopositive to 9 out of the 11 aforementioned antibodies and infected fish had a higher number of positive MCs when compared to uninfected fish.ConclusionsPike intestinal tissue response to A. lucii was documented. Numerous MCs were seen throughout the mucosa and submucosal layers. In infected and uninfected intestines of pike, MCs were the dominant immune cell type encountered; they are the most common granulocyte type involved in several fish-helminth systems. Immunopositivity of MCs to 9 out of 11 antibodies is of great interest and these cells could play an important key role in the host response to an enteric helminth. This is the first report of A. lucii in an Italian population of E. lucius and the first account on positivity of MCs to piscidin 3 and histamine in a non-perciform fish.

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Role of histamine in the regulation of intestinal immunity in fish

Cited by 33 publications

References 106 publications

Histopathological and ultrastructural assessment of two mugilid species infected with myxozoans and helminths

Histopathological and ultrastructural assessment of two mugilid species infected with myxozoans and helminths

Nematode infection in liver of the fish Gymnotus inaequilabiatus (Gymnotiformes: Gymnotidae) from the Pantanal Region in Brazil: pathobiology and inflammatory response

Pike intestinal reaction to Acanthocephalus lucii (Acanthocephala): immunohistochemical and ultrastructural surveys

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