2002
DOI: 10.1126/science.1076997
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Role of Histone H3 Lysine 27 Methylation in Polycomb-Group Silencing

Abstract: Polycomb group (PcG) proteins play important roles in maintaining the silent state of HOX genes. Recent studies have implicated histone methylation in long-term gene silencing. However, a connection between PcG-mediated gene silencing and histone methylation has not been established. Here we report the purification and characterization of an EED-EZH2 complex, the human counterpart of the Drosophila ESC-E(Z) complex. We demonstrate that the complex specifically methylates nucleosomal histone H3 at lysine 27 (H3… Show more

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Cited by 3,349 publications
(2,881 citation statements)
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References 35 publications
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“…The supernatant comprised the sheared chromatin for immune-precipitation and a fraction (10%) set aside as input chromatin prior to antibody affinity manipulations and subsequent reversal of crosslinking. The histone modification assessed on the basis of their antibody affinity were those specific for active gene expression, H3K9Ac [30] and for DNA methylation-independent gene silencing H3K27Me3 [31]. Antibodies were obtained from Abcam (Cambridge, UK) and have been described previously [19].…”
Section: Bisulphite Conversion and Pyrosequencingmentioning
confidence: 99%
“…The supernatant comprised the sheared chromatin for immune-precipitation and a fraction (10%) set aside as input chromatin prior to antibody affinity manipulations and subsequent reversal of crosslinking. The histone modification assessed on the basis of their antibody affinity were those specific for active gene expression, H3K9Ac [30] and for DNA methylation-independent gene silencing H3K27Me3 [31]. Antibodies were obtained from Abcam (Cambridge, UK) and have been described previously [19].…”
Section: Bisulphite Conversion and Pyrosequencingmentioning
confidence: 99%
“…Such caused the stabilization of Snail via activation of PI3K/AKT signaling and ultimately inhibited E-cadherin expression; EZH2 was also detected at the PTEN promoter locus (Song et al, 2009). In addition, the repressive function of PRC2 is required for PRC1 recruitment to target genes (Cao et al, 2002;Boyer et al, 2006;Simon and Lange, 2008), and PRC2 inactivation by Suz12 siRNA was found to increase mRNA levels of PTEN (Herranz et al, 2008;Song et al, 2009). These results suggest that EZH2 might also downregulate PTEN and consequently involve the PI3K/AKT/Snail pathway to repress E-cadherin.…”
Section: Ezh2 Promotes Npc Cell Aggressiveness Z-t Tong Et Almentioning
confidence: 99%
“…Les études initiales ont ainsi montré que la triméthylation de l'histone H3K27, via la protéine EZH2, pouvait servir de plateforme de recrutement à certaines protéines du complexe PRC1 (les protéines PC [polycomb] chez la drosophile et Cbx [chromobox] chez les mammifères) [16]. Par la suite, d'autres études ont montré que la perte de l'activité enzymatique d'EZH2 entraînait une importante diminution de liaison de PRC1 à l'ADN [16,17]. Un modèle hiérarchique a ainsi été Modèle hiérarchique selon lequel PRC2 est recruté à la chromatine et catalyse, via EZH2, l'apposition de la marque H3K27me3 qui conduit au recrutement de PRC1.…”
Section: Mécanismes De Recrutement Des Protéines Pcg à La Chromatineunclassified