Role of HLA-G 14bp deletion/insertion and +3142C&gt;G polymorphisms in the production of sHLA-G molecules in relapsing-remitting multiple sclerosis

Rizzo, Roberta; Bortolotti, Daria; Fredj, Nadia Ben; Rotola, Antonella; Curá, Francesca Maria; Castellazzi, Massimiliano; Tamborino, Carmine; Seraceni, Silva; Baldi, Eleonora; Melchiorri, Loredana; Tola, Maria Rosaria; Granieri, Enrico; Baricordi, Olavio R.; Fainardi, Enrico

doi:10.1016/j.humimm.2012.08.005

Cited by 52 publications

(54 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…sHLA-G levels in epithelial cell culture supernatants were assayed in triplicate as previously reported [29–31] using, as capture antibody, the monoclonal antibody (MoAb) MEM-G9 (Exbio), which recognizes the HLA-G molecule, in β 2-microglobulin associated form. The intra-assay coefficient of variation (CV) was 1.4% and the interassay CV was 4.0%.…”

Section: Methodsmentioning

confidence: 99%

Infection and HLA-G Molecules in Nasal Polyposis

Rizzo

Mozzillo²,

Bortolotti

et al. 2014

Journal of Immunology Research

Self Cite

View full text Add to dashboard Cite

Sinonasal polyposis (SNP) is a chronic inflammatory pathology with an unclear aetiopathogenesis. Human papillomavirus (HPV) infection is one candidate for the development of SNP for its epithelial cell trophism, hyperproliferative effect, and the induction of immune-modulatory molecules as HLA-G. We enrolled 10 patients with SNP without concomitant allergic diseases (SNP-WoAD), 10 patients with SNP and suffering from allergic diseases (SNP-WAD), and 10 control subjects who underwent rhinoplasty. We analyzed the presence of high- and low-risk HPV DNA and the expression of membrane HLA-G (mHLA-G) and IL-10 receptor (IL-10R) and of soluble HLA-G (sHLA-G) and IL-10 by polyp epithelial cells. The results showed the presence of HPV-11 in 50% of SNP-WoAD patients (OR:5.5), all characterized by a relapsing disease. HPV-11 infection was absent in nonrelapsing SNP-WoAD patients, in SNP-WAD patients and in controls, supporting the hypothesis that HPV-11 increases risk of relapsing disease. HPV-11 positive SNP-WoAD patients presented with mHLA-G and IL-10R on epithelial cells from nasal polyps and showed secretion of sHLA-G and IL-10 in culture supernatants. No HLA-G expression was observed in HPV negative polyps. These data highlight new aspects of polyposis aetiopathogenesis and suggest HPV-11 and HLA-G/IL-10 presence as prognostic markers in the follow-up of SNP-WoAD.

show abstract

Section: Methodsmentioning

confidence: 99%

Infection and HLA-G Molecules in Nasal Polyposis

Rizzo

Mozzillo²,

Bortolotti

et al. 2014

Journal of Immunology Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…The HLA-G 14 bp polymorphism was genotyped by polymerase chain reaction. 23,24 Soluble HLA-G detection by enzyme-linked immunosorbent assay sHLA-G1 and HLA-G5 levels were measured as reported previously. 25 After both enzyme-linked immunosorbent assay (ELISA) measurements, the amount of sHLA-G1 was expressed as the difference between sHLA-G1/HLA-G5 and HLA-G5 concentrations.…”

Section: Typing the Hla-g 14 Base Pair Polymorphismmentioning

confidence: 99%

HLA-G is a component of the chronic lymphocytic leukemia escape repertoire to generate immune suppression: impact of the HLA-G 14 base pair (rs66554220) polymorphism

et al. 2013

View full text Add to dashboard Cite

show abstract

“…In the present study, the 3 0 UTR polymorphisms 14 bp insertion/deletion (rs1704) and C3142COG SNP (rs1063320) that control HLA-G expression (21,22) were studied for the first time in AITD. Indeed, because high levels of HLA-G expression seem to be crucial for successful implantation, we hypothesized that a high secretor genetic profile in the mother could be associated with a higher tolerance of fetal cells and is thus associated with higher levels of FMC.…”

Section: European Journal Of Endocrinologymentioning

confidence: 99%

“…Indeed, HLA-G molecules are non-classical HLA class I antigens that are expressed by villous trophoblasts and are involved in the reprogramming of the local maternal immune response because they inhibit the activation of decidual T and NK cells; they have been proposed to positively influence the outcome of pregnancy (19,20). The HLA-G expression profile can be modulated by genetic polymorphisms, mainly two variants at the 3 0 UTR, the 14 bp insertion/deletion (rs1704), and the C3142COG (rs1063320), which influence mRNA stability and protein production and thereby lead to increased/reduced HLA-G expression and soluble HLA-G protein amounts in body fluids (21,22,23). In particular, the 14 bp deletion in hetero-or homozygosis is associated with a higher HLA-G expression (high secretor profile), whereas both the 14 bp insertion and the C3142COG polymorphism in exon 8 decrease HLA-G expression (low secretor profile).…”

Section: Introductionmentioning

confidence: 99%

Fetal cell microchimerism: a protective role in autoimmune thyroid diseases

Cirello

Rizzo

Crippa

et al. 2015

European Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

Objective: The physiological persistence of fetal cells in the circulation and tissue of a previously pregnant woman is called fetal cell microchimerism (FCM). It has been hypothesized to play a role in systemic autoimmune disease; however, only limited data are available regarding its role in autoimmune thyroid disease (AITD). Design: Circulating FCM was analyzed in a large series of previously pregnant women with Graves' disease (GD), Hashimoto's thyroiditis (HT), or no disease (healthy controls (HCs)). To exclude the possible bias related to placental factors, the polymorphic pattern of human leukocyte antigen-G (HLA-G) gene, which is known to be involved in the tolerance of fetal cells by the maternal immune system, was investigated. Methods: FCM was evaluated by PCR in the peripheral blood, and the Y chromosome was identified by fluorescence in situ hybridization in some GD tissues. HLA-G polymorphism typing was assessed by real-time PCR. Results: FCM was significantly more frequent in HC (63.6%) than in GD (33.3%) or HT (27.8%) women (PZ0.0004 and PZ0.001 respectively). A quantitative analysis confirmed that circulating male DNA was more abundant in HC than it was in GD or HT. Microchimeric cells were documented in vessels and in thyroid follicles. In neither GD/HT patients nor HC women was the HLA-G typing different between FCM-positive and FCM-negative cases. Conclusion: The higher prevalence of FCM in HC as compared to GD and HT patients suggests that it plays a possible protective role in autoimmune thyroid disorders. Placental factors have been excluded as determinants of the differences found. The vascular and tissue localization of microchimeric cells further highlights the ability of those cells to migrate to damaged tissues.

show abstract

Role of HLA-G 14bp deletion/insertion and +3142C>G polymorphisms in the production of sHLA-G molecules in relapsing-remitting multiple sclerosis

Cited by 52 publications

References 34 publications

Infection and HLA-G Molecules in Nasal Polyposis

Infection and HLA-G Molecules in Nasal Polyposis

HLA-G is a component of the chronic lymphocytic leukemia escape repertoire to generate immune suppression: impact of the HLA-G 14 base pair (rs66554220) polymorphism

Fetal cell microchimerism: a protective role in autoimmune thyroid diseases

Contact Info

Product

Resources

About