Role of horizontally transferred copper resistance genes in Staphylococcus aureus and Listeria monocytogenes

Kaur, Inderpreet; Purves, Joanne; Harwood, Matthew; Ketley, Julian M.; Andrew, Peter W.; Waldron, Kevin J.; Morrissey, Julie A.

doi:10.1099/mic.0.001162

Cited by 8 publications

(10 citation statements)

References 94 publications

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“…The TolC efflux pump was shown to be important in mitigating copper sensitivity. The use of copper can lead to resistance, with horizontal transfer of genes encoding for copper resistance reported in S. aureus and Listeria monocytogenes 150 .…”

Section: Antibacterial Strategies Based On Metal Uptakementioning

confidence: 99%

Metals to combat antimicrobial resistance

et al. 2023

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Bacteria, similar to most organisms, have a love–hate relationship with metals: a specific metal may be essential for survival yet toxic in certain forms and concentrations. Metal ions have a long history of antimicrobial activity and have received increasing attention in recent years owing to the rise of antimicrobial resistance. The search for antibacterial agents now encompasses metal ions, nanoparticles and metal complexes with antimicrobial activity (‘metalloantibiotics’). Although yet to be advanced to the clinic, metalloantibiotics are a vast and underexplored group of compounds that could lead to a much-needed new class of antibiotics. This Review summarizes recent developments in this growing field, focusing on advances in the development of metalloantibiotics, in particular, those for which the mechanism of action has been investigated. We also provide an overview of alternative uses of metal complexes to combat bacterial infections, including antimicrobial photodynamic therapy and radionuclide diagnosis of bacterial infections.

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Section: Antibacterial Strategies Based On Metal Uptakementioning

confidence: 99%

Metals to combat antimicrobial resistance

et al. 2023

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“…Resistance to heavy metals in S. aureus and other Staphylococcus spp. has been frequently reported [ 110 , 111 , 112 ]. All 66 heavy metal resistance plasmids in this study harbored cadmium resistance genes with the majority being cadAC ( n = 46) and cadDX ( n = 26), while only two plasmids encoded the czcD gene ( Figure 7 ), which has also been implicated in resistance towards cobalt and zinc ions [ 113 ].…”

Section: Resultsmentioning

confidence: 99%

The Plasmidomic Landscape of Clinical Methicillin-Resistant Staphylococcus aureus Isolates from Malaysia

et al. 2023

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Methicillin-resistant Staphylococcus aureus (MRSA) is a priority nosocomial pathogen with plasmids playing a crucial role in its genetic adaptability, particularly in the acquisition and spread of antimicrobial resistance. In this study, the genome sequences of 79 MSRA clinical isolates from Terengganu, Malaysia, (obtained between 2016 and 2020) along with an additional 15 Malaysian MRSA genomes from GenBank were analyzed for their plasmid content. The majority (90%, 85/94) of the Malaysian MRSA isolates harbored 1–4 plasmids each. In total, 189 plasmid sequences were identified ranging in size from 2.3 kb to ca. 58 kb, spanning all seven distinctive plasmid replication initiator (replicase) types. Resistance genes (either to antimicrobials, heavy metals, and/or biocides) were found in 74% (140/189) of these plasmids. Small plasmids (<5 kb) were predominant (63.5%, 120/189) with a RepL replicase plasmid harboring the ermC gene that confers resistance to macrolides, lincosamides, and streptogramin B (MLSB) identified in 63 MRSA isolates. A low carriage of conjugative plasmids was observed (n = 2), but the majority (64.5%, 122/189) of the non-conjugative plasmids have mobilizable potential. The results obtained enabled us to gain a rare view of the plasmidomic landscape of Malaysian MRSA isolates and reinforces their importance in the evolution of this pathogen.

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“…Heavy metals (arsenic, copper) are used as different biocides like herbicides, insecticides and fungicides, where they were used as plant growth regulators (arsenic) or fertilisers (cadmium, copper), and can be general feed contaminants (mercury, cadmium, tellurium) [77][78][79]. Industrialization, agriculture, and human and veterinary medicine have led to further widespread environmental contamination by heavy metals [80]. Pathogenic bacteria also encounter heavy metals as a host defence mechanism against invading microorganisms.…”

Section: Discussionmentioning

confidence: 99%

Plasmid-encoded lactose metabolism and mobilized colistin resistance (mcr-9) genes in Salmonella enterica serovars isolated from dairy facilities in the 1980s

Kröger,

Lerminiaux,

Ershova

et al. 2023

Microbial Genomics

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Horizontal gene transfer by plasmids can confer metabolic capabilities that expand a host cell’s niche. Yet, it is less understood whether the coalescence of specialized catabolic functions, antibiotic resistances and metal resistances on plasmids provides synergistic benefits. In this study, we report whole-genome assembly and phenotypic analysis of five Salmonella enterica strains isolated in the 1980s from milk powder in Munich, Germany. All strains exhibited the unusual phenotype of lactose-fermentation and encoded either of two variants of the lac operon. Surprisingly, all strains encoded the mobilized colistin resistance gene 9 (mcr-9), long before the first report of this gene in the literature. In two cases, the mcr-9 gene and the lac locus were linked within a large gene island that formed an IncHI2A-type plasmid in one strain but was chromosomally integrated in the other strain. In two other strains, the mcr-9 gene was found on a large IncHI1B/IncP-type plasmid, whereas the lac locus was encoded on a separate chromosomally integrated plasmidic island. The mcr-9 sequences were identical and genomic contexts could not explain the wide range of colistin resistances exhibited by the Salmonella strains. Nucleotide variants did explain phenotypic differences in motility and exopolysaccharide production. The observed linkage of mcr-9 to lactose metabolism, an array of heavy-metal detoxification systems, and other antibiotic resistance genes may reflect a coalescence of specialized phenotypes that improve the spread of colistin resistance in dairy facilities, much earlier than previously suspected.

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Role of horizontally transferred copper resistance genes in Staphylococcus aureus and Listeria monocytogenes

Cited by 8 publications

References 94 publications

Metals to combat antimicrobial resistance

Metals to combat antimicrobial resistance

The Plasmidomic Landscape of Clinical Methicillin-Resistant Staphylococcus aureus Isolates from Malaysia

Plasmid-encoded lactose metabolism and mobilized colistin resistance (mcr-9) genes in Salmonella enterica serovars isolated from dairy facilities in the 1980s

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