Role of Hydration State and Thiol-Disulfide Status in the Control of Thermal Stability and Protein Synthesis in Wheat Embryo

Fahey, Robert C.; Stefano, Deena L. Di; Meier, Gregor; Bryan, R. Nick

doi:10.1104/pp.65.6.1062

Cited by 53 publications

(23 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result reveals the function and efficiency of the GR enzyme (Fig. This relationship is consistent with the changes in the GSH levels found in aged tomato (De Vos et al 1994) and sunflower seeds (De Paula et al 1996), with rapid reduction of GSSG upon imbibition of isolated wheat embryos (Fahey et al 1980) and in imbibing pea seeds (Kranner and Grill 1993), and with the changes detected in GR activity (Klapheck et al 1990, Cakmak et al 1993, Bailly et al 1996. Thus, activation of the GSSG-reducing system, together with the removal of MDA, generates a more reduced environment that promotes normal cell function.…”

supporting

confidence: 85%

Ageing-induced changes in glutathione system of sunflower seeds

Torres¹,

Paula²,

Perez-Otaola³

et al. 1997

Physiol Plant

View full text Add to dashboard Cite

Ageing-induced changes in glutathione system of sunflower seeds. -Physiol. Plant. 101: 807-814.The glutathione system is thought to be involved in defence mechanisms present in plant tissues. The efficacy of this system was evaluated in large seeds of sunflower {Helianthus annuus L. cv. Peredovik) in response to accelerated ageing (43°C/75% relative humidity from 1 to 11 days). Differences between the embryo axis and cotyledons in relation to the glutathione system were also investigated. Additionally, lipid peroxidation was determined by measuring the malondialdehyde (MDA) content. All assays were performed using dry seeds and seeds subsequently hydrated by imbibition in distilled water for 12 h at 25°C. Accelerated ageing caused a marked decrease in seed viability, accompanied by an increase in mean germination time. There were no changes in total glutathione in dry seeds. However, the distribution in its reduced (GSH) and oxidized (GSSG) forms revealed that ageing produced a slow conversion from GSH to GSSG. As the ageing period increased, this effect was accompanied by a decrease in glutathione reductase (GR, EC 1.6.4.2) activity. The results also indicated that the GSH system exerts a different response in the embryo axis as compared with the cotyledon: (1) the GSH levels decreased less in the cotyledons than in axes of aged seeds, and (2) the GSSG level in cotyledons was independent of ageing, while its amount increased in aged embryo axes. These different responses, in conjunction with the lower MDA levels in large as compared with small seeds, indicate a possible protective role of the reserve lipids. The efficacy of the GSH system in aged seeds was associated with seed viability, as revealed by multiple regression analysis. Upon imbibition, aged seeds were able to restore their GSH levels, reaching values approximating those of unaged seeds.

show abstract

supporting

confidence: 85%

Ageing-induced changes in glutathione system of sunflower seeds

Torres¹,

Paula²,

Perez-Otaola³

et al. 1997

Physiol Plant

View full text Add to dashboard Cite

show abstract

“…Higher amounts of GSH and GSSG in dry embryos compared to germinating seeds were also noted which prevents further germination process (Tommasi et al 2001). Fahey et al (1980) also suggested that presence of GSSG in dormant wheat embryos block protein synthesis. These reports strongly supports our results that higher amounts of GSH and GSSG in non-embryogenic callus may be due to protein synthesis blockage preventing further development leading to yellowish brown callus.…”

Section: Resultsmentioning

confidence: 95%

Glutathione metabolism and antioxidant responses during Eleutherococcus senticosus somatic embryo development in a bioreactor

Shohael

Ali²,

Hahn³

et al. 2007

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Compared to non-embryogenic callus, proembryonic mass, globular, and heart-shaped embryos of Eleutherococcus senticosus had higher levels of endogenous reduced glutathione (GSH). GSH content declined during the course of the embryo development (torpedo and cotyledon). Similarly, glutathione reductase that is involved in the recycling of GSH providing a constant intracellular level of GSH was also higher in globular and heartshaped embryos. The transient increase in GSH contents also correlated with the changes in measured c-glutamylcysteine synthetase activity over the same period. The endogenous levels of oxidized glutathione showed similar trend during development of the somatic embryos, whereas it declined in maturing somatic embryos. A pronounced increase in glutathione-S-transferase, glutathione peroxidase, catalase, and guaiacol peroxidase activity was observed during somatic embryo maturation. Ascorbate-glutathione cycle enzymes (ascorbate peroxidase; dehydroascorbate reductase and monodehydroascorbate reductase) activities also induced indicated that antioxidant enzymes played an important role during embryo development. These results suggested that the coordinated up-regulations of the antioxidant enzymes and glutathione redox system provide protection during somatic embryo development in E. senticosus. Antioxidant responses through alterations of the glutathione redox systems, have been described in the present studies have a significant role in somatic embryo development.

show abstract

“…Mixed disulphide formation was also observed during desiccation of wheat grains, along with a decrease in the GSH/GSSG ratio and GR activity (Rhazi et al 2003). Rehydration of wheat embryos resulted in rapid reduction of PSSG within the first 10 mins of imbibition (Fahey et al 1980).…”

Section: S-glutathionylation In Stressed Plantsmentioning

confidence: 95%

Desiccation tolerant plants as model systems to study redox regulation of protein thiols

Colville

Kranner

2010

Plant Growth Regul

View full text Add to dashboard Cite

While the majority of plants and animals succumb to water loss, desiccation tolerant organisms can lose almost all of their intracellular water and revive upon rehydration. Only about 300 'resurrection' angiosperms and very few animals are desiccation tolerant. By contrast, many bryophytes and most lichens are desiccation tolerant and so are the seeds and pollen grains of most flowering plants. The current literature reveals that the extreme fluctuations in water content experienced by desiccation tolerant organisms are accompanied by equally extreme changes in cellular redox state. Strongly oxidizing conditions upon desiccation can cause irreversible oxidation of free cysteine residues of proteins, which can change protein structure and function, and contribute to protein denaturation. It appears likely that reversible formation of disulphide bonds, in particular through protein glutathionylation, contributes to the set of protection mechanisms that confer desiccation tolerance. Upon rehydration, de-glutathionylation can be catalyzed by glutaredoxins (GRXs) and protein disulphide bonds can be reduced through NADPH-dependent thioredoxins (TRXs). Due to their ability to survive severe oxidative stress, desiccation tolerant plants and seeds are excellent models to study protein redox regulation, which may provide tools for enhancing tolerance to drought and more generally, to oxidative stress, in crops.

show abstract

Role of Hydration State and Thiol-Disulfide Status in the Control of Thermal Stability and Protein Synthesis in Wheat Embryo

Cited by 53 publications

References 13 publications

Ageing-induced changes in glutathione system of sunflower seeds

Ageing-induced changes in glutathione system of sunflower seeds

Glutathione metabolism and antioxidant responses during Eleutherococcus senticosus somatic embryo development in a bioreactor

Desiccation tolerant plants as model systems to study redox regulation of protein thiols

Contact Info

Product

Resources

About