Role of Hydrogen Sulfide in Inflammatory Bowel Disease

Stummer, Nathalie; Feichtinger, René G.; Weghuber, Daniel; Kofler, Barbara; Schneider, Anna-Maria

doi:10.3390/antiox12081570

Cited by 20 publications

(6 citation statements)

References 221 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We identified six categories of amino acid degradation pathways that are relevant to host health because they produce compounds that are toxic, anti-inflammatory, neurotransmitters, or otherwise related to disease. The toxic compounds include ammonia produced by deaminating enzymes and urease 29,30 , as well as hydrogen sulfide produced by cysteine desulfurase 31 . The neurotransmitters include γ-aminobutyric acid (GABA) produced by glutamate decarboxylase 32 , and indoles produced by tryptophanase 33 ).…”

Section: Resultsmentioning

confidence: 99%

Dietary protein source strongly alters gut microbiota composition and function

Blakeley-Ruiz,

Bartlett,

McMillan

et al. 2024

Preprint

View full text Add to dashboard Cite

The source of protein in a persons diet affects their total life expectancy. However, the mechanisms by which dietary protein sources differentially impact human health and life expectancy are poorly understood. Dietary choices have major impacts on the composition and function of the intestinal microbiota that ultimately mediate host health. This raises the possibility that health outcomes based on dietary protein sources might be driven by interactions between dietary protein and the gut microbiota. In this study, we determine the effects of seven different sources of dietary protein on the gut microbiota in mice. We apply an integrated metagenomics-metaproteomics approach to simultaneously investigate the effects of these dietary protein sources on the gut microbiotas composition and function. The protein abundances measured by metaproteomics can provide microbial species abundances, and evidence for the phenotype of microbiota members on the molecular level because measured proteins allow us to infer the metabolic and physiological processes used by a microbial community. We showed that dietary protein source significantly altered the species composition and overall function of the gut microbiota. Different dietary protein sources led to changes in the abundance of microbial amino acid degrading proteins and proteins involved in the degradation of glycosylations on dietary protein. In particular, brown rice and egg white protein increased the abundance of amino acid degrading enzymes and egg white protein increased the abundance of bacteria and proteins usually associated with the degradation of the intestinal mucus barrier. These results show that dietary protein source can change the gut microbiotas metabolism, which could have major implications in the context of gut microbiota mediated diseases.

show abstract

Section: Resultsmentioning

confidence: 99%

Dietary protein source strongly alters gut microbiota composition and function

Blakeley-Ruiz,

Bartlett,

McMillan

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Therefore, our data suggest that LPD might enhance the gut's deconjugation of primary bile acids, offering potential protective mechanisms against inflammation and helping to restore a healthier microbial balance/composition. While the role of H2S has not been previously investigated in PSC, more has been done to investigate its role in UC with a small but largely supportive literature [48,49]. An increase in sulfate-reducing bacteria has been associated with UC and flares in particular [50,51].…”

Section: Discussionmentioning

confidence: 99%

“…While the role of H 2 S has not been previously investigated in PSC, more has been done to investigate its role in UC with a small but largely supportive literature [48, 49]. An increase in sulfate-reducing bacteria has been associated with UC and flares in particular [50, 51].…”

Section: Discussionmentioning

confidence: 99%

Demonstrating the Beneficial Effect of Low Protein Diet in Primary Sclerosing Cholangitis through a Randomized Clinical Trial and Multi-omics Data Analysis

Yin,

Sasson,

Sun

et al. 2024

Preprint

View full text Add to dashboard Cite

Primary sclerosing cholangitis (PSC), a progressive cholestatic hepatobiliary disease characterized by inflammation and fibrosis of the bile ducts, has a pathophysiology that is not understood. No effective therapies exist. The only treatment option for PSC is liver transplant. We undertook a pilot randomized trial of diet to investigate the pathophysiology of the disease, the role of diet and to advance potential therapy. We enrolled 20 patients with PSC and randomly assigned them to a Low Protein/low sulfur Diet (LPD, n=10) or the Specific Carbohydrate Diet (SCD, n=10) for 8 weeks. Results showed that low protein intake benefits PSC patients, whereas higher protein levels exacerbate the condition. We further identified gut bacterial markers useful for distinguishing LPD responders (mostly PSC with concomitant ulcerative colitis) from non-responders. Additionally, by integrating multi-omics data, we propose that this diet modifies the intestinal sulfur cycle reducing hydrogen sulfide (H2S) production. Our findings provide an understanding of the beneficial effect of LPD as well as insights into a possible key driver of inflammation in PSC.

show abstract

“…Studies in pigs have implicated Desulfovibrio as a major contributor in the utilization of feces for H 2 S production ( 77 ). And, H 2 S has been hypothesized to contribute to intestinal diseases such as inflammatory bowel disease ( 78 ), particularly ulcerative colitis ( 79 ). The RNA-seq results seemed to reflect the effect of harmful genera on ileal function.…”

Section: Discussionmentioning

confidence: 99%

Effects of rearing systems (cage versus floor) on the microbial composition and transcriptome of goose ileum

He,

Li,

Zhang

et al. 2024

Front. Vet. Sci.

View full text Add to dashboard Cite

There is a gradual transition from water to dryland rearing of geese. In this study, we performed 16S rRNA sequencing (16S rRNA-seq) and transcriptome sequencing (RNA-seq) to reveal the effects of cage rearing (CR) and floor rearing (FR) systems on the microbial composition and transcriptome of the goose ileum. Through 16S rRNA-seq, Linear Discriminant Analysis Effect Size (LEfSe) analysis identified 2 (hgcI_clade and Faecalibacterium) and 14 (Bacteroides, Proteiniphilum, Proteiniclasticum, etc.) differential microbiota in CR and FR, respectively. The rearing system influenced 4 pathways including biosynthesis of amino acids in ileal microbiota. Moreover, we identified 1,198 differentially expressed genes (DEGs) in the ileum mucosa, with 957 genes up-regulated in CR and 241 genes up-regulated in FR. In CR, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed the significant enrichment (p < 0.05) of 28 KEGG pathways, most of which were associated with amino acid metabolism. In FR, up-regulated DEGs were mainly enriched in KEGG pathways associated with cellular processes, including apoptosis, necroptosis, and cellular senescence. Spearman correlation analysis of differential microbiota and amino acid metabolism-related DEGs in CR showed a significant positive correlation. Additionally, differential microbiota of FR, Phascolarctobacterium and Sutterella, were positively correlated with FGF10 (p < 0.05) and PIK3R1 (p < 0.01), respectively. In conclusion, there might be differences in ileal amino acid metabolism levels between CR and FR geese, and the observed increase in harmful bacterial species in FR might impact the activity of ileal cells.

show abstract

Role of Hydrogen Sulfide in Inflammatory Bowel Disease

Cited by 20 publications

References 221 publications

Dietary protein source strongly alters gut microbiota composition and function

Dietary protein source strongly alters gut microbiota composition and function

Demonstrating the Beneficial Effect of Low Protein Diet in Primary Sclerosing Cholangitis through a Randomized Clinical Trial and Multi-omics Data Analysis

Effects of rearing systems (cage versus floor) on the microbial composition and transcriptome of goose ileum

Contact Info

Product

Resources

About