Role of <i>IFNLR1</i> gene in PRRSV infection of PAM cells

Qin, Ming; Chen, Wei; Li, Zhixin; Wang, Lixue; Ma, Lixia; Geng, Jinhong; Zhang, Yu; Zhao, Jing; Zeng, Yongqing

doi:10.4142/jvs.2021.22.e39

Cited by 4 publications

(2 citation statements)

References 47 publications

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“…Specifically, cytokines can bind to the corresponding receptors to stimulate JAK, followed by STAT dimerization in response to JAK phosphorylation, which then crosses the nuclear membrane to regulate the expression of immune-related genes in the nucleus. Several studies have pointed out that after activation of the JAK-STAT pathway, up-regulated STAT1 inhibits macrophage proliferation during porcine reproductive and respiratory syndrome virus infection [ 45 ], activation of STAT3 benefits respiratory function and reduces hepatocyte injury in pigs [ 46 ] and STAT5 has a regulatory role in human complement regulatory protein CD46 on T cell proliferation and immunity in response to porcine aortic endothelial cells [ 47 ]. Notably, three STAT proteins were enriched in the KEGG pathway of the “JAK-STAT signaling pathway”, and the results showed that they were significantly expressed in the livers of SN and LZ Tibetan pigs compared to JZT, with no significant difference in SNT/LZT ( Figure 7 B).…”

Section: Discussionmentioning

confidence: 99%

Quantitative Proteomic Analysis of Tibetan Pig Livers at Different Altitudes

Chang

Yang

et al. 2023

Molecules

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In this study, the differences in protein profiles between the livers of Shannan Tibetan pigs (SNT), Linzhi Tibetan pigs (LZT) and Jiuzhaigou Tibetan pigs (JZT) were comparatively analyzed by tandem mass spectrometry-labeling quantitative proteomics. A total of 6804 proteins were identified: 6471 were quantified and 1095 were screened as differentially expressed proteins (DEPs). Bioinformatics analysis results show that, compared with JZT livers, up-regulated DEPs in SNT and LZT livers mainly promoted hepatic detoxification through steroid hormone biosynthesis and participated in lipid metabolism to maintain body energy homeostasis, immune response and immune regulation, while down-regulated DEPs were mainly involved in lipid metabolism and immune regulation. Three proteases closely related to hepatic fatty acid oxidation were down-regulated in enzymatic activity, indicating higher levels of lipid oxidation in SNT and LZT livers than in JZT livers. Down-regulation of the expression of ten immunoglobulins suggests that JZT are more susceptible to autoimmune diseases. It is highly likely that these differences in lipid metabolism and immune-related proteins are in response to the ecological environment at different altitudes, and the findings contribute to the understanding of the potential molecular link between Tibetan pig livers and the environment.

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Section: Discussionmentioning

confidence: 99%

Quantitative Proteomic Analysis of Tibetan Pig Livers at Different Altitudes

Chang

Yang

et al. 2023

Molecules

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“…Dapulian pigs are recognized for their strong immune system and their resistance to pathogens [54]. Interestingly, it was reported that the expression of the IFN-λs receptor 1 (IFNLR1) gene in the lung of Dapulian pigs is significantly higher than in Landrace pigs and that this difference makes the former more resistant to PRRSV infection [55]. Then, the higher expression of IRF3, IFN-β and IFN-λ1 found in porcine alveolar macrophages treated with HK36, HK39 or HK40 allow us to speculate that these postimmunobiotics could have beneficial effects in the protection of the respiratory tract against viral infections in the porcine host.…”

Section: Discussionmentioning

confidence: 99%

Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity

Tomokiyo

Tonetti

Yamamuro

et al. 2022

Cells

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Beneficial microbes with immunomodulatory capacities (immunobiotics) and their non-viable forms (postimmunobiotics) could be effectively utilized in formulations towards the prevention of respiratory viral infections. In this study, novel immunobiotic strains with the ability to increase antiviral immunity in porcine alveolar macrophages were selected from a library of Lactobacillus gasseri. Postimmunobiotics derived from the most remarkable strains were also evaluated in their capacity to modulate the immune response triggered by Toll-like receptor 3 (TLR3) in alveolar macrophages and to differentially regulate TLR3-mediated antiviral respiratory immunity in infant mice. We provide evidence that porcine alveolar macrophages (3D4/31 cells) are a useful in vitro tool for the screening of new antiviral immunobiotics and postimmunobiotics by assessing their ability to modulate the expression IFN-β, IFN-λ1, RNAseL, Mx2, and IL-6, which can be used as prospective biomarkers. We also demonstrate that the postimmunobiotics derived from the Lactobacillus gasseri TMT36, TMT39 and TMT40 (HK36, HK39 or HK40) strains modulate the innate antiviral immune response of alveolar macrophages and reduce lung inflammatory damage triggered by TLR3 activation in vivo. Although our findings should be deepened and expanded, the results of the present work provide a scientific rationale for the use of nasally administered HK36, HK39 or HK40 to beneficially modulate TLR3-triggerd respiratory innate immune response.

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