Role of<i>Rhizobium</i>Cellulase CelC2 in Host Root Colonization and Infection

Garrido, Marta; Rivera, Lina P.; Menéndez, Esther; Martínez‐Hidalgo, Pilar; Rivas, Raúl; Velázquez, Encarna; Dazzo, Frank B.; Martínez‐Molina, Eustoquio; Mateos, Pedro F.

doi:10.1002/9781119053095.ch53

Cited by 6 publications

(1 citation statement)

References 33 publications

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“…A comparison of the physiological features of rhizobia with those of other endophytes indicates that rhizobia do not have as many potentially beneficial features as endophytes. Most rhizobia have a relatively high cellulase production potential, and it is possible that this facilitates colonization of plant tissues (Robledo et al, 2015). On the other hand, in the group of 29 rhizobial strains, production of siderophores, production of IAA, phosphate solubilization, and indole synthesis were found only in 4, 4, 1, and 1 strain, respectively.…”

Section: Discussionmentioning

confidence: 99%

Genetic and physiological diversity of white Spanish broom (Chamaecytisus albus) endophytes

et al. 2021

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Chamaecytisus albus (Spanish broom) is a legume shrub that can be found in only one natural locality in Poland. This specimen is critically endangered; therefore, different actions focusing on protection of this plant in the natural habitat are undertaken, and one of them involves studies of the population of Chamaecytisus albus bacterial endophytes, which in the future could be used as bioprotectants and/or biofertilizers. A collection of 94 isolates was obtained from Spanish broom nodules, and the physiological and genetic diversity of these strains was studied. A few potentially beneficial traits were detected, i.e. secretion of cellulases (66 isolates), production of siderophores (60 isolates), phosphate solubilization (25 isolates), and production of IAA (58 isolates), indole (16 isolates), or HCN (3 isolates). Twenty-nine of the 94 tested isolates were able to induce the development of root nodules in plants grown in vitro and can therefore be assumed as Chamaecytisus albus symbionts. Genome fingerprinting by BOX-PCR, as well as gyrB and nodZ gene sequencing revealed a great genetic diversity of specimens in the collection. The symbiotic isolates were classified in different clades, suggesting they could belong to different species, however, most of them revealed sequence similarity to Bradyrhizobium genus.

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Section: Discussionmentioning

confidence: 99%

Genetic and physiological diversity of white Spanish broom (Chamaecytisus albus) endophytes

et al. 2021

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GunA of Sinorhizobium (Ensifer) fredii HH103 is a T3SS-secreted cellulase that differentially affects symbiosis with cowpea and soybean

et al. 2018

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The symbiosis between Sinorhizobium fredii HH103 and its host legumes is influenced by the type 3 secretion system (T3SS), which delivers proteins (effectors) directly into the host cells to promote infection. GunA, one of the predicted HH103 effectors, potentially codes for a cellulase. In this work we tried to characterise GunA and elucidate its role in symbosis with soybean and cowpea. MethodsA GunA::HA fusion protein was constructed to study T3SS-dependent secretion.Cellulase activity of GunA was measured and gunA::uidA-GFP and gunA::cyA fusions were constructed to monitor gunA expression in nodules and to study translocation to the host cells, respectively. Finally, the symbiotic performance of a gunA mutant was studied in soybean and cowpea. ResultsGunA from S. fredii HH103 shows cellulase activity and is secreted through the T3SS in response to the inducer flavonoid genistein. Interestingly, at the beginning of the symbiotic process, GunA was partially responsible for the induction of the expression of the soybean GmPR1 gene, a gene used as a marker for plant defense responses.However, GunA was also detected in soybean and cowpea developed nodules. Finally, nodulation assays indicate that GunA is beneficial for symbiosis with soybean but detrimental with cowpea. Conclusion

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Calcium in the Life Cycle of Legume Root Nodules

Zartdinova,

Nikitin

2023

Indian J Microbiol

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Role ofRhizobiumCellulase CelC2 in Host Root Colonization and Infection

Cited by 6 publications

References 33 publications

Genetic and physiological diversity of white Spanish broom (Chamaecytisus albus) endophytes

Genetic and physiological diversity of white Spanish broom (Chamaecytisus albus) endophytes

GunA of Sinorhizobium (Ensifer) fredii HH103 is a T3SS-secreted cellulase that differentially affects symbiosis with cowpea and soybean

Calcium in the Life Cycle of Legume Root Nodules

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