Role of Immunocompetent Cells in the Regulation of Human Megakaryocytopoiesis In Vitro

Detrick, Robert A.; Schulman, Jeanne C.; Mamus, S W; McEver, R.P.; Zanjani, Esmail D.

doi:10.1007/978-1-4684-5571-7_23

Cited by 1 publication

(1 citation statement)

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“…Consistent with our results, increased levels of IFN-γ, but not of IL-4, have been reported in patients with chronic ITP [35], along with elevated IL-17 plasma levels [36]. An in vitro study reported that the addition of IFN-γ to the BM inhibited human megakaryocytopoiesis [37], which suggests that elevated levels of these cytokines may adversely affect PLT production in patients with PT.…”

Section: Discussionsupporting

confidence: 90%

Abnormalities of the Bone Marrow Immune Microenvironment in Patients with Prolonged Isolated Thrombocytopenia after Allogeneic Hematopoietic Stem Cell Transplantation

Song

Shi

Zhang

et al. 2017

Biology of Blood and Marrow Transplantation

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Prolonged isolated thrombocytopenia (PT) is a serious complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Whether abnormalities of the bone marrow (BM) immune microenvironment are involved in the pathogenesis of PT remains unknown, however. Twenty patients with PT, 40 matched patients with good graft function (GGF) after allo-HSCT, and 20 healthy donors (HD) were enrolled in this nested case-control study. Th1, Th2, Tc1, Tc2, Th17, and Treg cells were analyzed by flow cytometry, and IFN-γ, IL-4, IL-17, IL-6, IL-21, and thrombopoietin levels in BM plasma were evaluated with a cytometric bead assay and ELISA. Relative to GGF patients and HD controls, PT patients had significantly higher proportions of Th1 and Tc1 cells, resulting in higher Th1/Th2 and Tc1/Tc2 ratios in the BM microenvironment. In addition, the excessive polarization of Th17 was observed in patients with PT. Changes in BM plasma cytokines were consistent with our cellular findings. These results suggest that dysregulated T cell responses in the BM microenvironment might play an important role in the pathogenesis of PT.

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Section: Discussionsupporting

confidence: 90%