Role of Inducible Nitric Oxide Synthase and Interleukin-6 Proteins Expression in Estimation of Skin Burn Age and Vitality: Immunohistochemical Study in Rat

Abstract

All rights reserved. Introduction ound examination is an essential issue in forensic practice. Determination of wound age and vitality is a classic but still popular and pivotal issue in forensic pathology to evaluate accurately its causal relationship to death (Kubo, 2014). Wound healing is a dynamic process consisting of three overlapping phases: the inflammatory phase; including coagulation and inflammation, the proliferative phase; consisting of angiogenesis, the formation of granulation tissue, and re-epithelialization, and lastly the maturation phase; involving matrix formation and tissue remodeling (Mendonça and Coutinho-Netto, 2009). There are many studies investigated determination of mechanically induced skin wounds by sharp or blunt objects using either animal experiments or samples from cadavers (Takamiya et al., 2002; Kondo, 2007; Kondo and Ishida, 2010). Despite burn injuries are considered a major cause of disability and death (Alsarhan et al., 2013); limited information is available on the determination of skin burn injury.