Role of interleukin 10 in murine postoperative ileus

Abstract

IL10 plays an obligatory role in postoperative intestinal recovery, and exogenous IL10 prevents its development. Pre-emptive exogenous recombinant human IL10 could be a treatment for the prevention of clinical POI.

Anti-inflammatory Effect of Ginsenoside Rb1 Contributes to the Recovery of Gastrointestinal Motility in the Rat Model of Postoperative Ileus

“…24) In addition, it is reported that IL-10 deficiency could lead to a prolonged gastrointestinal dysmotility after surgery and abnormal high gene expression of pro-inflammatory cytokines in the animal model of POI. 39) In the present study, we found that POI rats showed significantly higher levels of pro-inflammatory cytokine TNF-α, IL-1β and IL-6 in serum and the ileum tissue, while these elevated levels were significantly reduced by GRb1 in a dose-dependent manner, as well as their gene expression in the ileum tissue. In addition, although the level of anti-inflammatory cytokine IL-10 in serum and the ileum tissue was significantly increased in POI rats, GRb1 also continuously increased this elevated level in a dose-dependent manner, as well as this gene expression in the ileum tissue.…”

Immune mediators of postoperative ileus

“…Most analyses were done on the transcriptional level, demonstrating a rapid induction of several genes immediately after intestinal manipulation. Others presented detailed expression time curves of up to 20 different mediators measured within an ELISA or multiplex protein assay [39,41] that were detected either in muscularis culture supernatants or lysates. Interestingly, POI development is obviously driven by a T H 1 immunoresponse as alterations of the prototypical T H 2 cytokines IL-4, IL-5, or IL-13 have not been found.…”

“…Interestingly, POI development is obviously driven by a T H 1 immunoresponse as alterations of the prototypical T H 2 cytokines IL-4, IL-5, or IL-13 have not been found. [39,41] While most works solely demonstrated expression profiles of selected cytokines, recent publications pointed out functionally significance of individual cytokines in the pathogenesis of POI. In 2005, our group demonstrated that IL-6 is a major cytokine in POI development that leads to nuclear activation of the STAT3 transcription factor [42] which is involved in counter regulatory antiinflammatory signaling.…”

“…Another prominent proinflammatory cytokine is IL-12 that is upregulated after intestinal manipulation [26,41]. Importantly, IL-12 release from CD103 + CD11b + dendritic cells promotes activation of macrophages with subsequent IFN-γ release and migration of T H 1 memory which is an early event in POI development and dissemination along the gastrointestinal tract.…”

Chronic Kidney Disease Elicits an Intestinal Inflammation Resulting in Intestinal Dysmotility Associated with the Activation of Inducible Nitric Oxide Synthesis in Rat

“…However, the lack of cytokine IL-10 will also directly inhibit intestinal motility. Therefore, all these cytokines are often employed to assess the status of intestinal inflammation and the related intestinal dysmotlity [34,37,38]. In the present study, we determined these cytokines and found that CKD induced a progressive increase in the intestinal concentrations of TNF-α, IL-6, and IL-10 at all investigated time points, reaching statistical significance level on postoperative week 8 and/or 10, as well as the difference for gene expression on postoperative weeks 6, 8, and 10.…”