Role of interleukin-4 in human immunoglobulin E formation in hu-PBL-SCID mice.

Spiegelberg, Hans L.; Beck, Lucinda; Kocher, Hans P.; Fanslow, William C.; Lucas, Alexander H.

doi:10.1172/jci117024

Cited by 35 publications

(23 citation statements)

References 28 publications

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“…In contrast, Ito et al [3] showed that with peripheral blood lymphocytes (PBLs) from atopic do nors, reconstituted mice produced IgE, perhaps because B cells from atopies more easily switched to IgE that do B cells from nonatopic donors. Moreover, human IgE formation in SCID mice was Interleukin (IL)-4 dependent: incubation of PBLs with IL-4 before reconstitution enhanced IgE produc tion 10-fold [4], In our study, SCID mice, reconstituted with PBMCs of patients allergic to D.pt. also exhibited a signif icant human IgE response; this IgE production was exclu sively obtained after reconstitution in animals immunized with the related allergen [5], When stimulation was done with an unrelated allergen (grass pollen or Hymenoptera venom), we were unable to induce IgE formation.…”

Section: Discussionmentioning

confidence: 64%

Human IgE in Severe Combined Immunodeficiency Mice Reconstituted with Peripheral Blood Mononuclear Cells from Dermatophagoides pteronyssinus-Sensitive Patients

Tonnel

Pestel²,

Duez³

et al. 1995

Int Arch Allergy Immunol

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We studied conditions of human IgE formation in severe combined immunodeficiency (SCID) mice engrafted with peripheral blood mononuclear cells (PBMCs) from allergic patients sensitive to Dermatophagoides pteronyssinus (D.pt.). With 10 × 10⁶ PBMCs injected intraperitoneally, the hu-SCID mice developed an IgE response, but only if experimental animals were immunized with the related allergen. Two routes of immunization were tested: intraperitoneal and inhalation. In these experimental conditions (allergen given at day 14 after reconstitution), a significant rise in total serum IgE but also in specific anti-D.pt. IgE was observed. No human IgE could be detected within 3–4 weeks after immunization with an unrelated allergen. Similarly, when mice were engrafted with PBMCs from nonallergic donors, even after D.pt. administration, no significant increase of serum IgE was detectable, while an IgG response was regularly found. Thus SCID mice could represent a useful model to analyze IgE production as well as the conditions of immunization required to obtain an optimal response.

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Section: Discussionmentioning

confidence: 64%

Human IgE in Severe Combined Immunodeficiency Mice Reconstituted with Peripheral Blood Mononuclear Cells from Dermatophagoides pteronyssinus-Sensitive Patients

Tonnel

Pestel²,

Duez³

et al. 1995

Int Arch Allergy Immunol

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“…Humanized SCID mice can produce a specific human IgG response against recall or naive antigens [8,11,12, 181 and human IgE in the absence [13,14] or in the presence of relevant allergen [15]. However, in these latter studies, the protocol used to induce the IgE response was not similar to the natural route of allergen sensitization in humans.…”

Section: Discussionmentioning

confidence: 99%

“…When peripheral blood cells from vaccinated donors are used, the immunized Hu-SCID mice develop an Ag-specific IgG antibody response [ 11, 121. Recently, SCID mice reconstituted i.p. with 5 x lo7 PBMC from atopic patients were shown spontaneously to produce human IgE [13,14]. In our laboratory, Ag-specific IgE production has been demonstrated in SCID mice reconstituted i.p.…”

Section: Introductionmentioning

confidence: 99%

An in vivo model of allergic inflammation: pulmonary human cell infiltrate in allergen‐challenged allergic Hu‐SCID mice

et al. 1996

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CB.17 severe combined immunodeficient (SCID) mice were used to establish a model of allergic pulmonary inflammation. SCID mice were intraperitoneally reconstituted with 10(7) peripheral blood mononuclear cells (PBMC) from patients sensitive to Dermatophagoides pteronyssinus (Dpt) and 2 weeks later were exposed to Dpt aerosols. After Dpt nebulization, SCID mice engrafted with PBMC from Dpt-sensitive patients developed a specific human IgE response as well as an intense pulmonary infiltrate of human cells. In contrast, SCID mice reconstituted with PBMC from patients allergic to grass pollen or from non-allergic donors failed to produce IgE or to exhibit a similar inflammatory response. The level of the IgE production was dependent on the IgE level of the allergic donor. In the lungs of the same allergic SCID mice, 2 months after Dpt inhalation, the cell infiltrate contained CD45+, CD45RO+, CD20+ and HLA-DR+ human cells. They were located in perivascular and peribronchial areas and disseminated in the mouse lung parenchyma. Moreover, mRNA IL-5+ cells and eosinophils were found in peribronchial infiltrates. The observations indicate that humanized allergic SCID mice may develop, after nebulization with the relevant allergen, immune reactions similar to those observed in man and suggest that SCID mice may represent a useful model to analyze the regulatory mechanisms of IgE-associated allergic diseases.

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“…Although the relationship between the production of IgG subclasses and T helper (Th) cytokines in humans is not as well defined as in the mouse, certain cytokines have been implicated in the switch to particular IgG subclasses. Thus, the presence of certain IgG subclasses can serve as an indication of Th1 or Th2 cytokines produced at the time of class switching and IgG production [13][14][15][16]. Th cells play an important role in balancing cellular (Th1 dominant) and humoral (Th2 dominant) immune effector functions by producing different combinations of cytokines and/or crossregulation between different cytokines.…”

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confidence: 99%

Type Specificity and Significance of Different Isotypes of Serum Antibodies to Human Papillomavirus Capsids

Wang¹,

Kjellberg²,

Abdalla³

et al. 2000

J INFECT DIS

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Isotype-specific serum antibody responses against human papillomavirus (HPV) type 16 were evaluated by use of cross-sectional, prospective, and population-based seroepidemiologic studies. IgG1 and IgA were the most abundant isotypes. No sample contained IgG2, and <25 samples contained IgG3 or IgM. Total IgG, IgA, and IgG1 were HPV type specific and were associated with HPV-16 DNA (odds ratios [ORs], 5.4, 5.0, and 5.9, respectively; P<.001) but not with other HPV DNA (ORs, 1.2, 1.2, and 0.8, respectively; P value was not significant). Total IgG and IgG1 were strongly associated with number of lifetime sex partners (P<.001); IgA was only associated with number of recent sex partners and lifetime sex partners among younger women. Total IgG, IgG1, and IgA were associated with cervical intraepithelial neoplasia type III and also predicted risk of future cervical neoplasia. IgG and IgG1 appeared to mark lifetime cumulative exposure, whereas IgA may mark recent or ongoing infection.

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Role of interleukin-4 in human immunoglobulin E formation in hu-PBL-SCID mice.

Cited by 35 publications

References 28 publications

Human IgE in Severe Combined Immunodeficiency Mice Reconstituted with Peripheral Blood Mononuclear Cells from <i>Dermatophagoides pteronyssinu</i><i>s</i>-Sensitive Patients

Human IgE in Severe Combined Immunodeficiency Mice Reconstituted with Peripheral Blood Mononuclear Cells from <i>Dermatophagoides pteronyssinu</i><i>s</i>-Sensitive Patients

An in vivo model of allergic inflammation: pulmonary human cell infiltrate in allergen‐challenged allergic Hu‐SCID mice

Type Specificity and Significance of Different Isotypes of Serum Antibodies to Human Papillomavirus Capsids

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