Role of interleukin-6 in antigen-specific mucosal immunoglobulin A induction by cationic liposomes

Tada, Rui; Hidaka, Akira; Tanazawa, Yuya; Ohmi, Akari; Muto, Shoko; Ogasawara, Miki; Saito, Momoko; Ohshima, Akihiro; Iwase, Naoko; Honjo, Emi; Kiyono, Hiroshi; Kunisawa, Jun; Negishi, Yoichi

doi:10.1016/j.intimp.2021.108280

Cited by 6 publications

(2 citation statements)

References 43 publications

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“…Therefore, consistent with previous studies, the ratio of CD19 + B cells determined the reaction after COVID-19 vaccination, and a high percentage of CD19 + B cells was positively correlated with the concentration of NAbs. In addition, the current study showed that IL-6 cytokines in the volunteers were correlated with the production of NAbs [ 23 ]. IL-6 is a cytokine expressed at the earliest stage of tissue injury and infection that induces the synthesis of C-reactive protein (CRP) and fibrinogen in the acute phase response.…”

Section: Discussionmentioning

confidence: 99%

Clinical and laboratory features in health care volunteers with inactivated SARS-CoV-2 vaccination

LI,

WAN,

et al. 2023

Turkish Journal of Medical Sciences

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Background/aim To better optimize the inactivated vaccine-induced immune response and improve vaccine protection efficiency, a preliminary study was conducted on the influencing factors of producing neutralizing antibody (NAb) titers against the inactivated coronavirus disease 2019 (COVID-19) vaccine. Materials and methods A total of 91 health care volunteers were enrolled from the Immunology Division of the Laboratory Department of Chongqing General Hospital from February to March 2021. The study had a cross-sectional design. All of the volunteers were scheduled to receive a complete dose regimen of the inactivated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine and the vaccination interval between 2 doses was 14 days. Clinical and laboratory features were collected for further analysis. Results The NAb titers gradually increased after COVID-19 vaccination, and 72.53% (n = 66) of the volunteers had NAbs after the second dose. Eight variables, including CD16 + CD56 + NK cell level before the first dose (HR = 0.94, p = 0.02), CD16 + CD56 + NK cell level after the second dose (HR = 0.94, p = 0.03), interleukin (IL)-2 level before the first dose (HR = 2.09, p = 0.05), mean corpuscular volume (HR = 0.86, p = 0.02), serum urea level (HR = 0.69, p = 0.05), increment of CD19 + B cells (HR = 0.86, p = 0.03), increment of CD4 + /CD8 + T cells (HR = 0.21, p = 0.03), and increment of the IL-6 level (HR = 0.75, p = 0.04) demonstrated a correlation with the NAb titers after COVID-19 vaccination. In the multivariate logistical regression analysis, the serum urea level (HR = 2.32, P = 0.03) and increment of CD19 + B cells (HR = 1.96, p = 0.03) were positively correlated with the NAb titers. The principal component analysis effectively distinguished the response after COVID-19 vaccination. The Pearson correlation analysis indicated that the CD19 + B cell level (r = 0.23, p < 0.001) and IL-2 (r = 0.24, p < 0.001) and IL-6 levels (r = 0.22, p < 0.001) were weakly positively correlated with the concentration of NAbs. Conclusion The NAbs titers of the inactivated vaccines were positively correlated with the ratio of CD19 + B cell, IL-6, and IL-2 levels in the serum, which provide clinical guidance for inactivated SARS-CoV-2 vaccines.

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Section: Discussionmentioning

confidence: 99%

Clinical and laboratory features in health care volunteers with inactivated SARS-CoV-2 vaccination

LI,

WAN,

et al. 2023

Turkish Journal of Medical Sciences

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“…BALB/cCrSlc female mice were pre-treated with anti-IL-6R antibody or isotype control antibody (250 µg/mouse) two days prior to the first immunization (day-2) and 1 h prior to each subsequent immunization (days 0 and 7), as described in an earlier study [17]. The mice were nasally immunized with PBS (vehicle) or OVA (5 µg/mouse) with CpG ODN-loaded cationic liposomes (10 µg as CpG ODN/mouse) at a volume of 13 µL on days 0 and 7 under anesthesia with an intraperitoneal injection of 0.2 mL of a mixture containing 0.75 mg/kg of medetomidine, 4 mg/kg of midazolam, and 5 mg/kg of butorphanol tartrate.…”

Section: Immunization Schedule and Blocking Effect Of Anti-il-6r Anti...mentioning

confidence: 99%

Role of Interleukin-6 in the Antigen-Specific Mucosal Immunoglobulin A Responses Induced by CpG Oligodeoxynucleotide-Loaded Cationic Liposomes

et al. 2022

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An advantage of mucosal vaccines over conventional parenteral vaccines is that they can induce protective immune responses not only at mucosal surfaces but also in systemic compartments. Despite this advantage, few live attenuated or inactivated mucosal vaccines have been developed and applied clinically. We recently showed that the intranasal immunization of ovalbumin (OVA) with class B synthetic oligodeoxynucleotides (ODNs) containing immunostimulatory CpG motif (CpG ODN)-loaded cationic liposomes synergistically exerted both antigen-specific mucosal immunoglobulin A (IgA) and systemic immunoglobulin G (IgG) responses in mice. However, the mechanism underlying the mucosal adjuvant activity of CpG ODN-loaded liposomes remains unknown. In the present study, we showed that the intranasal administration of CpG ODN-loaded cationic liposomes elicited interleukin (IL)-6 release in nasal tissues. Additionally, pre-treatment with an anti-IL-6 receptor (IL-6R) antibody attenuated antigen-specific nasal IgA production but not serum IgG responses. Furthermore, the intranasal administration of OVA and CpG ODN-loaded cationic liposomes increased the number of IgA+/CD138+ plasma cells and IgA+/B220+ B cells in the nasal passages. This increase was markedly suppressed by pre-treatment with anti-IL-6R blocking antibody. In conclusion, IL-6 released by CpG ODN-loaded cationic liposomes at the site of administration may play a role in the induction of antigen-specific IgA responses by promoting differentiation into IgA+ plasma cells for IgA secretion from B cells.

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Probiotic Potential of Bacillus sp. 62A Isolated from a Marine Extreme Environment

Angulo,

Guerra,

Arevalo

et al. 2023

Probiotics & Antimicro. Prot.

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Role of interleukin-6 in antigen-specific mucosal immunoglobulin A induction by cationic liposomes

Cited by 6 publications

References 43 publications

Clinical and laboratory features in health care volunteers with inactivated SARS-CoV-2 vaccination

Clinical and laboratory features in health care volunteers with inactivated SARS-CoV-2 vaccination

Role of Interleukin-6 in the Antigen-Specific Mucosal Immunoglobulin A Responses Induced by CpG Oligodeoxynucleotide-Loaded Cationic Liposomes

Probiotic Potential of Bacillus sp. 62A Isolated from a Marine Extreme Environment

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