Role of Ion–Phospholipid Interactions in Zwitterionic Phospholipid Bilayer Ion Permeation

Deplazes, Evelyne; Tafalla, Beatriu Domingo; Cranfield, Charles G.; García, Álvaro

doi:10.1021/acs.jpclett.0c01479

Cited by 18 publications

(23 citation statements)

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“…Results show that the addition of Ca 2+ over 6 orders of magnitude in concentrations ranging from 10 uM to 1 M causes the normalized conductance to increase only 1.5 times (Figure A). In contrast, our previous tBLM/EIS experiments with NaCl and KCl reported increases in membrane conductance of between 5 and 10 times over the same concentration range . This result correlates well with the previous reports of low membrane permeability of Ca 2+ . − …”

Section: Resultssupporting

confidence: 91%

“…To further understand the interactions of Ca 2+ with phospholipid bilayers on a structural level, we carried out MD simulations of POPC in the presence of 500 mM and 1 M CaCl 2 . As reference systems, previous simulations of POPC only and POPC with 1 M NaCl or 1 M KCl were used . Simulations were carried out using the ECC force field parameters for POPC and ions.…”

Section: Resultsmentioning

confidence: 99%

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Calcium Ion Binding at the Lipid–Water Interface Alters the Ion Permeability of Phospholipid Bilayers

et al. 2021

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Calcium ions (Ca 2+ ) play a fundamental role in membrane-associated physiological processes. Ca 2+ can also significantly modulate the physicochemical properties of phospholipid bilayers, but whether this occurs at physiologically relevant concentrations is difficult to determine because of the uncertainty in the reported affinity of Ca 2+ for phospholipid bilayers. In this article, we determine the apparent affinity of Ca 2+ for zwitterionic phospholipid bilayers using tethered bilayer lipid membranes (tBLMs) used in conjunction with swept-frequency electrical impedance spectroscopy (EIS). We report that Ca 2+ binds to phospholipid bilayers at physiologically relevant concentrations and modulates membrane permeability. We present direct experimental evidence that this effect is governed by specific interactions with select lipid headgroup moieties, which is supported by data from molecular dynamics (MD) simulations. This is the first reported use of tBLM/EIS to estimate cation−membrane affinity. Combined with MD simulations, this technique provides a novel methodology to elucidate the molecular details of cation−membrane interactions at the water−phospholipid interface.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Calcium Ion Binding at the Lipid–Water Interface Alters the Ion Permeability of Phospholipid Bilayers

et al. 2021

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“…across the plasma membrane with moderate metabolic energy and fast migration speed. [30][31][32][33] Notably, the merits of high-efficiency ion transport and structural stability are the desired characteristics for AZIB cathode materials.…”

Section: Introductionmentioning

confidence: 99%

Construction of Bio‐inspired Film with Engineered Hydrophobicity to Boost Interfacial Reaction Kinetics of Aqueous Zinc–Ion Batteries

Gou

Luo

Zheng

et al. 2022

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Aqueous zinc–ion batteries typically suffer from sluggish interfacial reaction kinetics and drastic cathode dissolution owing to the desolvation process of hydrated Zn2+ and continual adsorption/desorption behavior of water molecules, respectively. To address these obstacles, a bio‐inspired approach, which exploits the moderate metabolic energy of cell systems and the amphiphilic nature of plasma membranes, is employed to construct a bio‐inspired hydrophobic conductive poly(3,4‐ethylenedioxythiophene) film decorating α‐MnO2 cathode. Like plasma membranes, the bio‐inspired film can “selectively” boost Zn2+ migration with a lower energy barrier and maintain the integrity of the entire cathode. Electrochemical reaction kinetics analysis and theoretical calculations reveal that the bio‐inspired film can significantly improve the electrical conductivity of the electrode, endow the cathode–electrolyte interface with engineered hydrophobicity, and enhance the desolvation behavior of hydrated Zn2+. This results in an enhanced ion diffusion rate and minimized cathode dissolution, thereby boosting the overall interfacial reaction kinetics and cathode stability. Owing to these intriguing merits, the composite cathode can demonstrate remarkable cycling stability and rate performance in comparison with the pristine MnO2 cathode. Based on the bio‐inspired design philosophy, this work can provide a novel insight for future research on promoting the interfacial reaction kinetics and electrode stability for various battery systems.

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“…1a). This allows tBLMs to mimic the fluidity of cell membranes [3] and peptides have sufficient space to form ion channels (Fig. 1b).…”

Section: Introductionmentioning

confidence: 99%

“…2). EIS can be used to monitor the change in membrane conductance (G m ) induced by different cations traversing the membrane in order to investigate the cation selectivity of peptide pores [3].…”

Section: Introductionmentioning

confidence: 99%

Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation Conductance Measures of Tethered Bilayer Lipid Membranes

Hartmann

García

Deplazes

et al. 2021

Methods in Molecular Biology

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Swept frequency electrical impedance spectroscopy (EIS) can be used in conjunction with tethered bilayer lipid membranes to monitor the membrane permeability of ions in real-time (Deplazes et al. J Phys Chem Lett 11:6353-6358, 2020). Conductance readings, as determined by EIS, are a measure of the ability of ions to be transported across membranes. Recording the change in conductance as a function of cation concentration and a comparison between a range of cations permits conclusions to be made about the specificity of cation transport through pores. An estimate for upper pore size and cation selectivity of ion channels can be established using this method.

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Role of Ion–Phospholipid Interactions in Zwitterionic Phospholipid Bilayer Ion Permeation

Cited by 18 publications

References 50 publications

Calcium Ion Binding at the Lipid–Water Interface Alters the Ion Permeability of Phospholipid Bilayers

Calcium Ion Binding at the Lipid–Water Interface Alters the Ion Permeability of Phospholipid Bilayers

Construction of Bio‐inspired Film with Engineered Hydrophobicity to Boost Interfacial Reaction Kinetics of Aqueous Zinc–Ion Batteries

Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation Conductance Measures of Tethered Bilayer Lipid Membranes

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