Role of irbesartan in protection against pulmonary toxicity induced by bleomycin in rats

El-Gamal, Mariam A.

doi:10.9790/3013-034203847

Cited by 4 publications

(2 citation statements)

References 44 publications

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“…Bleomycin (BLM) dissolved in normal saline and was given intraperitoneal (IP) in a dose of 10 mg/kg/day/10 days) to induce lung fibrosis [17]. Green tea extract (GET) was obtained from Technomade Group (Nasr City, Cairo, Egypt) as 350 gm powdered extract.…”

Section: Methodsmentioning

confidence: 99%

Modulatory effects of green tea and aloe vera extracts on experimentally-induced lung fibrosis in rats: histological and immunohistochemical study

Salem¹,

El-Azab²,

Faruk³

2014

J Histol Histopathol

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Background: Pulmonary fibrosis (PF) is a multifactorial disease in the human. The existing therapeutic agents display unfavorable adverse effects. Consequently, the discovery of novel therapeutic agents for the prevention and treatment of PF remains a challenge. Aim of the work: Evaluation of the modulatory effect of green tea and aloe vera (AV) extracts on bleomycin induced pulmonary fibrosis in rats. Material and methods: Fifty adult male rats were utilized and divided equally into 5 groups. The first was served as control group, the second was the bleomycin (BLM) group (daily I.P. dose 10 mg/kg body weight for 10 days), the third was the green tea (GTE) group which was given daily oral dose of GTE (150 mg/ kg b.w.) for 14 days with concomitant IP administration of BLM for 10 consecutive days, the fourth was the Aloe vera (AV) group which was given daily oral dose of AV (300 mg/kg b.w.) for 14 days with concomitant IP administration of BLM for 10 consecutive days, the fifth was the GTE and AV group. Lung samples were taken 14 days after the treatment. Paraffin sections were prepared for histological; H&E and Masson's trichrome staining and immunohistochemical study. Results: Green tea group revealed normal bronchioles, alveoli with apparently thin interalveolar septa, few cellular infiltration and extravasated RBCs and induced a significant decreased (P≤0.05) in collagen fibers accumulation and in caspase-3 expression compared with BLM group. Group IV (AV) and group V (GTE and AV) revealed alveoli with apparently thicker interalveolar septa, more cellular infiltration, congested blood vessels and extravasated RBCs in comparison with GTE group. Group IV showed insignificant decreased (P≤0.05) in collagen fibers accumulation compared with BLM group while the decrease was significant in caspase-3 expression. Group V showed insignificant decreased (P≤0.05) in collagen fibers accumulation and in caspase-3 expression compared with BLM group. Conclusion: Green tea and aloe vera partially alleviate pulmonary fibrosis induced by bleomycin in rats. Supporting the potential benefits of using aloe vera and green tea as a potential novel therapeutic agents by diet.

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Section: Methodsmentioning

confidence: 99%

Modulatory effects of green tea and aloe vera extracts on experimentally-induced lung fibrosis in rats: histological and immunohistochemical study

Salem¹,

El-Azab²,

Faruk³

2014

J Histol Histopathol

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“…An expert pathologist examined the slides in the dark using a light microscope (Olympus CX21, Tokyo, Japan) at ×40 magnification. The optical density of lung α-SMA was measured using the imageJ program, which was developed by the National Institute of Health in the United States [25,26].…”

Section: Immunohistochemical Staining and Image Analysis For α-Smamentioning

confidence: 99%

Synergistic Power of Piceatannol and/or Vitamin D in Bleomycin-Induced Pulmonary Fibrosis In Vivo: A Preliminary Study

Ezz Eldeen,

Moustafa,

Alwaili

et al. 2023

Biomedicines

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Oxidative stress and epigenetic alterations, including the overexpression of all class I and II histone deacetylases (HDACs), particularly HDAC2 and HDAC4, have been identified as key molecular mechanisms driving pulmonary fibrosis. Treatment with piceatannol (PIC) or vitamin D (Vit D) has previously exhibited mitigating impacts in pulmonary fibrosis models. The present study investigated the effects of PIC, Vit D, or a combination (PIC-Vit D) on the expression of HDAC2, HDAC4, and transforming growth factor-beta (TGF-β) in the lungs; the phosphatidylinositide-3-kinase (PI3K)/AKT signaling pathway; and the antioxidant status of the lungs. The objective was to determine if the treatments had protective mechanisms against pulmonary fibrosis caused by bleomycin (BLM) in rats. Adult male albino rats were given a single intratracheal dosage of BLM (10 mg/kg) to induce pulmonary fibrosis. PIC (15 mg/kg/day, oral (p.o.)), Vit D (0.5 μg/kg/day, intraperitoneal (i.p.)), or PIC-Vit D (15 mg/kg/day, p.o. plus 0.5 μg/kg/day, i.p.) were given the day following BLM instillation and maintained for 14 days. The results showed that PIC, Vit D, and PIC-Vit D significantly improved the histopathological sections; downregulated the expression of HDAC2, HDAC4, and TGF-β in the lungs; inhibited the PI3K/AKT signaling pathway; decreased extracellular matrix (ECM) deposition including collagen type I and alpha smooth muscle actin (α-SMA); and increased the antioxidant capacity of the lungs by increasing the levels of glutathione (GSH) that had been reduced and decreasing the levels of malondialdehyde (MDA) compared with the BLM group at a p-value less than 0.05. The concomitant administration of PIC and Vit D had a synergistic impact that was greater than the impact of monotherapy with either PIC or Vit D. PIC, Vit D, and PIC-Vit D exhibited a notable protective effect through their antioxidant effects, modulation of the expression of HDAC2, HDAC4, and TGF-β in the lungs, and suppression of the PI3K/AKT signaling pathway.

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Autophagy modulation by irbesartan mitigates the pulmonary fibrotic alterations in bleomycin challenged rats: Comparative study with rapamycin

et al. 2022

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Role of irbesartan in protection against pulmonary toxicity induced by bleomycin in rats

Cited by 4 publications

References 44 publications

Modulatory effects of green tea and aloe vera extracts on experimentally-induced lung fibrosis in rats: histological and immunohistochemical study

Modulatory effects of green tea and aloe vera extracts on experimentally-induced lung fibrosis in rats: histological and immunohistochemical study

Synergistic Power of Piceatannol and/or Vitamin D in Bleomycin-Induced Pulmonary Fibrosis In Vivo: A Preliminary Study

Autophagy modulation by irbesartan mitigates the pulmonary fibrotic alterations in bleomycin challenged rats: Comparative study with rapamycin

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