Role of Iron and Sulfur in Pigment and Slime Formation by
            <i>Pseudomonas aeruginosa</i>

Palumbo, Samuel A.

doi:10.1128/jb.111.2.430-436.1972

Cited by 27 publications

(10 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LMJ has light pink-brown pigmentation. Pseudomonas aeruginosa has water soluble pinkish red pyorubrin pigment and dark brown pyomelanin pigment [69][70][71] . Pyorubrin's biosynthesis is enhanced by the addition of 1% DL-glutamate in the growth media 69 .…”

Section: Pseudomonas Facilis Pseudomonas Delafieldii E Falsen (Ef)mentioning

confidence: 99%

See 1 more Smart Citation

Isolation and characterization of a novel bacterial strain from a Tris-Acetate-Phosphate agar medium plate of the green micro-alga Chlamydomonas reinhardtii that can utilize common environmental pollutants as a carbon source

et al. 2020

View full text Add to dashboard Cite

Background: Chlamydomonas reinhardtii, a green micro-alga can be grown at the lab heterotrophically or photo-heterotrophically in Tris-Phosphate-Acetate (TAP) medium which contains acetate as the sole carbon source. When grown in TAP medium, Chlamydomonas can utilize the exogenous acetate in the medium for gluconeogenesis using the glyoxylate cycle, which is also present in many bacteria and higher plants. A novel bacterial strain, LMJ, was isolated from a contaminated TAP medium plate of Chlamydomonas. We present our work on the isolation and physiological and biochemical characterizations of LMJ. Methods: Several microbiological tests were conducted to characterize LMJ, including its sensitivity to four antibiotics. We amplified and sequenced partially the 16S rRNA gene of LMJ. We tested if LMJ can utilize cyclic alkanes, aromatic hydrocarbons, poly-hydroxyalkanoates, and fresh and combusted car motor oil as the sole carbon source on Tris-Phosphate (TP) agar medium plates for growth. Results: LMJ is a gram-negative rod, oxidase-positive, mesophilic, non-enteric, pigmented, salt-sensitive bacterium. LMJ can ferment glucose, is starch hydrolysis-negative, and is very sensitive to penicillin and chloramphenicol. Preliminary spectrophotometric analyses indicate LMJ produces pyomelanin. NCBI-BLAST analyses of the partial 16S rRNA gene sequence of LMJ showed that it matched to that of an uncultured bacterium clone LIB091_C05_1243. The nearest genus relative of LMJ is an Acidovorax sp. strain. LMJ was able to use alkane hydrocarbons, fresh and combusted car motor oil, poly-hydroxybutyrate, phenanthrene, naphthalene, benzoic acid and phenyl acetate as the sole carbon source for growth on TP-agar medium plates. Conclusions: LMJ has 99.14% sequence identity with the Acidovorax sp. strain A16OP12 whose genome has not been sequenced yet. LMJ’s ability to use chemicals that are common environmental pollutants makes it a promising candidate for further investigation for its use in bioremediation and, provides us with an incentive to sequence its genome.

show abstract

Section: Pseudomonas Facilis Pseudomonas Delafieldii E Falsen (Ef)mentioning

confidence: 99%

“…Pyorubrin's biosynthesis is enhanced by the addition of 1% DL-glutamate in the growth media 69 . Pyorubrin has a characteristic absorption peak in the visible light at 520 nm 70 . Pyomelanin is produced as a product of tyrosine catabolism via homogentisate (HGA) (Figure 7).…”

Section: Pseudomonas Facilis Pseudomonas Delafieldii E Falsen (Ef)mentioning

confidence: 99%

Isolation and characterization of a novel bacterial strain from a Tris-Acetate-Phosphate agar medium plate of the green micro-alga Chlamydomonas reinhardtii that can utilize common environmental pollutants as a carbon source

et al. 2020

View full text Add to dashboard Cite

show abstract

“…One reason for the early interest in this pigment is the dramatic green color which it imparts to wounds (green pus or pyoverdin) and to culture media. Many of the early publications described the nutritional requirements for pyoverdin production (10, 14,18,29,33,36). Although many medium constituents were found to support pyoverdin production, it was notable that iron inhibited production (10, 13, 14, 19, 20, 22, 36).…”

Section: Cox and Adamsmentioning

confidence: 99%

Siderophore activity of pyoverdin for Pseudomonas aeruginosa

Cox¹,

Adams²

1985

Infect Immun

244

126

View full text Add to dashboard Cite

Pseudomonas aeruginosa produces an extracellular compound with yellowish green fluorescence, called pyoverdin, which functions as a siderophore. The production of pyoverdin, formerly called fluorescein, is concomitant with the production of another siderophore, pyochelin. Pyoverdin is produced by P. aeruginosa in several forms, some of which were separated on gel filtration columns and on reverse-phase, high-pressure liquid chromatography columns. An active form of iron-free pyoverdin was purified to homogeneity. The elution of pyoverdin from the columns was monitored for absorbance, fluorescence, and siderophore activities. These activities, iron binding, and the stimulation of bacterial iron transport indicated that pyoverdin can function as a siderophore for P. aeruginosa. The siderophore function of pyoverdin may be related to the pathogenicity of this bacterium because pyoverdin stimulated growth not only in iron-deficient culture medium, but also in defined medium containing transferrin and in human serum or plasma.

show abstract

“…Pigment formation and elaboration rely on a dynamic metabolic equilibrium provided by medium constituents such as peptones, minerals, and various ions. Inasmuch as this medium was empirically derived, magnesium, potassium, phosphate, and sulfates were incorporated according to guidelines established by the work of King et al (8) and others (3)(4)(5)(6)9). The precise function of these components is not known.…”

Section: Methodsmentioning

confidence: 99%

Differential primary plating medium for enhancement of pigment production by Pseudomonas aeruginosa

Daly¹,

Boshard²,

Matsen³

1984

J Clin Microbiol

View full text Add to dashboard Cite

A cost-effective and more rapid means of detection of Pseudomonas aeruginosa in cultures from clinical specimens would be very advantageous. We have developed a modified MacConkey agar (MMA), which enhances pigment production of P. aeruginosa and which, if pyocyanin pigment is present, provides a relatively rapid and very cost-effective identification. The MMA medium inhibits the gram-positive organisms, while lactose-and non-lactose-fermenting gram-negative rods are easily distinguishable from pigment-producing pseudomonads. Organisms that produce pyocyanin, pyoverdin, or pyorubin, or both pyocyanin and pyoverdin, are easily recognized on the medium. Pyocyanin production is clearly distinguishable from other Pseudomonas pigments on MMA. In a comparative study, MMA identified 97% of the P. aeruginosa strains 24 h earlier than routine laboratory biochemical methods. Highly mucoid strains which did not produce detectable pigments on standard biochemicals produced detectable pigments on the MMA within 48 h. This medium can provide a very practical, reliable, and cost-effective means for early characterization of P. aeruginosa.

show abstract

Role of Iron and Sulfur in Pigment and Slime Formation by Pseudomonas aeruginosa

Cited by 27 publications

References 29 publications

Isolation and characterization of a novel bacterial strain from a Tris-Acetate-Phosphate agar medium plate of the green micro-alga Chlamydomonas reinhardtii that can utilize common environmental pollutants as a carbon source

Isolation and characterization of a novel bacterial strain from a Tris-Acetate-Phosphate agar medium plate of the green micro-alga Chlamydomonas reinhardtii that can utilize common environmental pollutants as a carbon source

Siderophore activity of pyoverdin for Pseudomonas aeruginosa

Differential primary plating medium for enhancement of pigment production by Pseudomonas aeruginosa

Contact Info

Product

Resources

About