Role of Klotho and N-acetylcysteine in Oxidative Stress Associated with the Vitrification of Ovarian Tissue Cytoprotective Function of Klotho in Cryopreservation

Kim, Boram; Lee, Seon-Mi; Park, Sangjun; Lee, Sanghoon; Kim, Tak

doi:10.1007/s13770-023-00556-7

Cited by 5 publications

(1 citation statement)

References 27 publications

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“…Given the high similarity in composition and structure between tendon and ligament, it is reasonable to hypothesize that Klotho could induce similar biological effects in tendon as well. Furthermore, a prior investigation directly compared the effect of Klotho and N -acetylcysteine, a widely recognized potent antioxidant, and revealed that Klotho exhibited a similar capacity to mitigate DNA damage and cellular senescence by inhibiting oxidative stress, while inducing less cellular apoptosis in comparison to N -acetylcysteine ( 38 ). Together, the utilization of recombinant Klotho as an antioxidant and immunomodulatory biomolecule integrated into tissue engineering scaffolds holds promise to improve cell microenvironment of injured tissues, thereby facilitating the MTJ repair processes.…”

Section: Introductionmentioning

confidence: 99%

Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction

Sun,

Sheng,

Cao

et al. 2024

Sci. Adv.

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Myotendinous junction (MTJ) injuries are prevalent in clinical practice, yet the treatment approaches are limited to surgical suturing and conservative therapy, exhibiting a high recurrence rate. Current research on MTJ tissue engineering is scarce and lacks in vivo evaluation of repair efficacy. Here, we developed a three-dimensional–printed bioactive fiber-reinforced hydrogel containing mesenchymal stem cells (MSCs) and Klotho for structural and functional MTJ regeneration. In a rat MTJ defect model, the bioactive fiber-reinforced hydrogel promoted the structural restoration of muscle, tendon, and muscle-tendon interface and enhanced the functional recovery of injured MTJ. In vivo proteomics and in vitro cell cultures elucidated the regenerative mechanisms of the bioactive fiber-reinforced hydrogel by modulating oxidative stress and inflammation, thus engineering an optimized microenvironment to support the survival and differentiation of transplanted MSCs and maintain the functional phenotype of resident cells within MTJ tissues, including tendon/muscle cells and macrophages. This strategy provides a promising treatment for MTJ injuries.

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Section: Introductionmentioning

confidence: 99%