Role of kruppel-like transcription factors in adipogenesis

Wu, Zeni; Wang, Haihui

doi:10.1016/j.ydbio.2012.10.031

Cited by 124 publications

(112 citation statements)

References 114 publications

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“…For example, Zfp521 [30], Zfp423 [31], ZNF638 [32], ZNF395 [33], Snail [34] and kruppel-like transcription factors [35] were shown to be regulative in adipogenesis. In this group here, we detected eleven different ZFPs as well as Klf10, Klf9 and Klf5.…”

Section: Resultsmentioning

confidence: 99%

Dynamics of mRNA and polysomal abundance in early 3T3-L1 adipogenesis

et al. 2014

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BackgroundAdipogenesis is a complex process, in which immature pre-adipocytes change morphology, micro-anatomy and physiology to become mature adipocytes. These store and accumulate fat and release diverse hormones. Massive changes in protein content and protein composition of the transforming cell take place within a short time-frame.In a previous study we analyzed changes in the abundance of free and polysomal, i.e. ribosome bound, RNAs in the first hours of adipogenesis in the murine cell line 3T3-L1. Here we analyze changes of mRNA levels and their potential contribution to the changing protein pool by determination of mRNA levels and ribosome binding to mRNAs in 3T3-L1 cells stimulated for adipogenesis. We grouped mRNA species into categories with respect to up- or down-regulated transcription and translation and analyzed the groups regarding specific functionalities based on Gene Ontology (GO).ResultsA shift towards up-regulation of gene expression in early adipogenesis was detected. Genes up-regulated at the transcriptional (TC:up) and translational (TL:up) level (TC:up/TL:up) are very likely involved in control and logistics of translation. Many of them are known to contain a TOP motif. In the TC:up/TL:unchanged group we detected most of the metal binding proteins and metal transporters. In the TC:unchanged/TL:up group several factors of the olfactory receptor family were identified, while in TC:unchanged/TL:down methylation and repair genes are represented. In the TC:down/TL:up group we detected many signaling factors. The TC:down/TL:unchanged group mainly consists of regulatory factors.ConclusionsWithin the first hours of adipogenesis, changes in transcriptional and translational regulation take place. Notably, genes with a specific biological or molecular function tend to cluster in groups according to their transcriptional and translational regulation.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-381) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Dynamics of mRNA and polysomal abundance in early 3T3-L1 adipogenesis

et al. 2014

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“…KLFs constitute an interesting family of transcription factors involved in the regulation of diverse metabolic networks (Neve et al 2005;Lomberk et al 2013;Zhang et al 2013). Several KLF family members, including KLF2, KLF3, KLF4, KLF5, KLF6, KLF7, KLF9, and KLF15, have been implicated in different stages of adipogenesis (Wu and Wang 2013), but a role of KLF11 in adipogenesis has not been described. KLF11 has been shown to activate UCP1 expression in mouse bone marrow-derived stem cells (Yamamoto et al 2010); however, the impact of KLF11 on browning of adipocytes remains unknown.…”

Section: Discussionmentioning

confidence: 99%

Browning of human adipocytes requires KLF11 and reprogramming of PPARγ superenhancers

et al. 2014

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Long-term exposure to peroxisome proliferator-activated receptor g (PPARg) agonists such as rosiglitazone induces browning of rodent and human adipocytes; however, the transcriptional mechanisms governing this phenotypic switch in adipocytes are largely unknown. Here we show that rosiglitazone-induced browning of human adipocytes activates a comprehensive gene program that leads to increased mitochondrial oxidative capacity. Once induced, this gene program and oxidative capacity are maintained independently of rosiglitazone, suggesting that additional browning factors are activated. Browning triggers reprogramming of PPARg binding, leading to the formation of PPARg ''superenhancers'' that are selective for brown-in-white (brite) adipocytes. These are highly associated with key brite-selective genes. Based on such an association, we identified an evolutionarily conserved metabolic regulator, Kruppel-like factor 11 (KLF11), as a novel browning transcription factor in human adipocytes that is required for rosiglitazone-induced browning, including the increase in mitochondrial oxidative capacity. KLF11 is directly induced by PPARg and appears to cooperate with PPARg in a feed-forward manner to activate and maintain the brite-selective gene program.

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“…Since the first mammalian KLF, named KLF1/EKLF (erythroid Krüppel-like factor), was first identified as a master regulator of erythropoiesis [81], the KLF subfamily has reportedly been involved in various cellular processes, including cell proliferation, differentiation as well as apoptosis. Recently, a series of experiments demonstrated that many KLF members play vital roles in adipogenesis and adipose development (reviewed in [82, 83]) thus showing their potential therapeutic value in fighting against obesity. A total of 9 KLF members have been identified to be responsible for controlling white adipocyte development, of which KLF4, KLF5, KLF6, KLF8, KLF9, and KLF15 promote adipogenesis whereas KLF2, KLF3, and KLF7 inhibit adipogenesis.…”

Section: Zinc Finger Proteins In White Adipogenesismentioning

confidence: 99%

Emerging roles of zinc finger proteins in regulating adipogenesis

Wei

Zhang

Zhou

et al. 2013

Cell. Mol. Life Sci.

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Proteins containing the zinc finger domain(s) are named zinc finger proteins (ZFPs), which are one of the largest classes of transcription factors in eukaryotic genomes. A large number of ZFPs have been studied and many of them were found to be involved regulating normal growth and development of cells and tissues through diverse signal transduction pathways. Recent studies revealed that a small but increasing number of ZFPs could function as key transcriptional regulators involved in adipogenesis. As the prevalence of obesity and metabolic disorders, the investigation of molecular regulatory mechanisms of adipocyte development must be more completely understood to develop novel and long term impact strategies for ameliorating obesity. In this review, we discuss recent work which has documented that ZFPs are important functional contributors to the regulation of adipogenesis. Taken altogether these data lead to the conclusion that ZFPs may become promising targets to combat human obesity.

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Role of kruppel-like transcription factors in adipogenesis

Cited by 124 publications

References 114 publications

Dynamics of mRNA and polysomal abundance in early 3T3-L1 adipogenesis

Dynamics of mRNA and polysomal abundance in early 3T3-L1 adipogenesis

Browning of human adipocytes requires KLF11 and reprogramming of PPARγ superenhancers

Emerging roles of zinc finger proteins in regulating adipogenesis

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