Role of L1CAM in the Regulation of the Canonical Wnt Pathway and Class I MAGE Genes

Shkurnikov, M. Yu.; Knyazev, E N; Wicklein, Daniel; Schumacher, Udo; Samatov, Timur R.; Tonevitskii, A. G.

doi:10.1007/s10517-016-3315-4

Cited by 20 publications

(4 citation statements)

References 15 publications

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“…In a total of 19 studies investigated the impact of full-length L1CAM (L1CAM-FL) on tumor suppression with strategies that did not specifically address domain-specific functions of L1CAM [34,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61]. A complete overview of the main study strategies and findings is outlined in Table S1 and a summary of results can be found in Figure 3.…”

Section: Resultsmentioning

confidence: 99%

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L1 Cell Adhesion Molecule in Cancer, a Systematic Review on Domain-Specific Functions

Maten

Reijnen

Pijnenborg

et al. 2019

IJMS

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L1 cell adhesion molecule (L1CAM) is a glycoprotein involved in cancer development and is associated with metastases and poor prognosis. Cellular processing of L1CAM results in expression of either full-length or cleaved forms of the protein. The different forms of L1CAM may localize at the plasma membrane as a transmembrane protein, or in the intra- or extracellular environment as cleaved or exosomal forms. Here, we systematically analyze available literature that directly relates to L1CAM domains and associated signaling pathways in cancer. Specifically, we chart its domain-specific functions in relation to cancer progression, and outline pre-clinical assays used to assess L1CAM. It is found that full-length L1CAM has both intracellular and extracellular targets, including interactions with integrins, and linkage with ezrin. Cellular processing leading to proteolytic cleavage and/or exosome formation results in extracellular soluble forms of L1CAM that may act through similar mechanisms as compared to full-length L1CAM, such as integrin-dependent signals, but also through distinct mechanisms. We provide an algorithm to guide a step-wise analysis on L1CAM in clinical samples, to promote interpretation of domain-specific expression. This systematic review infers that L1CAM has an important role in cancer progression that can be attributed to domain-specific forms. Most studies focus on the full-length plasma membrane L1CAM, yet knowledge on the domain-specific forms is a prerequisite for selective targeting treatment.

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Section: Resultsmentioning

confidence: 99%

“…Activation of these pathways may also occur in parallel with ERK activation, for instance in ovarian carcinoma [55]. L1CAM is also reported to be associated with the Wnt/b-catenin pathway, both as target in colorectal carcinoma [26] but also as upstream regulator in melanoma [52] and breast cancer [44].…”

Section: Resultsmentioning

confidence: 99%

L1 Cell Adhesion Molecule in Cancer, a Systematic Review on Domain-Specific Functions

Maten

Reijnen

Pijnenborg

et al. 2019

IJMS

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“…Гибридизацию, промывание, окрашивание и сканирование микрочипов проводили по описанной ранее методике [11,12]. Обработку CEL-файлов, полученных при сканировании микрочипов, проводили с помощью программного пакета Transcriptome Analysis Console 2.0 [13]. Неаннотированные пробсеты (наборы проб на микрочипе) были исключены из анализа.…”

Section: оценка изменения профиля экспрессии генов с использованием микрочиповunclassified

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2019

Biiotehnologiâ (Mosk.)

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Исследовано влияние эндогенной экспрессии α5-цепи ламининов на фенотип клеток колоректального рака. Для этого был осуществлен нокдаун экспрессии гена LAMA5, кодирующего α5-цепь ламининов, в клетках линии колоректальной аденокарциномы НТ29. Данные изменения профиля экспрессии генов как на уровне мРНК, так и на уровне белка, свидетельствуют о вовлеченности α5-цепи ламининов в регуляцию процесса дифференцировки клеток. Так, наблюдалось снижение экспрессии таких маркеров дифференцировки эпителия кишечника, как SI, KRT20, ANXA13, MUC13. Кроме того детектировалось увеличение экспрессии маркеров стволовых клеток эпителия кишечника LRG5 и трансмембранного белка CD44. Изменение фенотипа клеток HT29 может быть следствием влияния α5-цепь ламининов на активность Wnt-сигнального пути.

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“…The RNA concentration was determined using a NanoDrop 1000 instrument [ 12 ]. The RNA quality was assessed using a Bio-Rad Experion instrument by the RQI value [ 13 ].…”

mentioning

confidence: 99%

Chemical Induction of Trophoblast Hypoxia by Cobalt Chloride Leads to Increased Expression of DDIT3

Knyazev

Paul

Tonevitsky

2021

Dokl Biochem Biophys

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Choriocarcinoma cells BeWo b30 are used to model human placental trophoblast hypoxia using cobalt (II) chloride and hydroxyquinoline derivative (HD) as chemical inducers of hypoxia-inducible factor (HIF). In this study, it was shown that both substances activate the hypoxic pathway and the epithelial–mesenchymal transition and inhibit the pathways of cell proliferation. However, CoCl2 caused activation of the apoptosis pathway, increased the activity of effector caspases 3 and 7, and increased the expression of the unfolded protein response target DDIT3. The mTORC1 pathway was activated upon exposition to CoCl2, while HD suppressed this pathway, as it happens during real trophoblast hypoxia. Thus, effect of CoCl2 on BeWo cells can be a model of severe hypoxia with activation of apoptosis, while HD mimics moderate hypoxia.

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Role of L1CAM in the Regulation of the Canonical Wnt Pathway and Class I MAGE Genes

Cited by 20 publications

References 15 publications

L1 Cell Adhesion Molecule in Cancer, a Systematic Review on Domain-Specific Functions

L1 Cell Adhesion Molecule in Cancer, a Systematic Review on Domain-Specific Functions

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Chemical Induction of Trophoblast Hypoxia by Cobalt Chloride Leads to Increased Expression of DDIT3

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