Role of lactosylceramide-enriched membrane microdomains in CD11b/CD18-mediated neutrophil phagocytosis

Nakayama, Hitoshi

doi:10.4052/tigg.20.259

Cited by 1 publication

(1 citation statement)

References 12 publications

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“…However, CD11b/CD18 does not possess a cytoplasmic signaling motif (which is typically required to aid in the self‐mediation of outside‐in signaling). Therefore, the mediation of CD11b/CD18‐dependent functions requires other PRRs and/or signaling molecules (Nakayama, ). Recently, Shimotahira et al () demonstrated that the S‐layer of T. forsythia contributes to serum resistance and coaggregation with other oral bacteria.…”

Section: Discussionmentioning

confidence: 99%

Energy dispersive spectroscopy‐scanning transmission electron microscope observations of free radical production in human polymorphonuclear leukocytes phagocytosing non‐opsonized Tannerella forsythia

Moriguchi

Hasegawa

Higuchi

et al. 2017

Microscopy Res & Technique

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We investigated the association between human polymorphonuclear leukocytes (PMNs) and non-opsonized Tannerella forsythia ATCC 43037 displaying a serum-resistant surface layer (S-layer). When PMNs were mixed with T. forsythia in suspension, the cells phagocytosed T. forsythia cells. Nitro blue tetrazolium (NBT) reduction, indicative of O2- production, was observed by light microscopy; cerium (Ce) perhydroxide deposition, indicative of H O production, was observed by electron microscopy. We examined the relationship between high-molecular-weight proteins of the S-layer and Ce reaction (for T. forsythia phagocytosis) using electron microscopic immunolabeling. Immunogold particles were localized within the PMNs and on cell surfaces, labelling at the same Ce-reacted sites where the S-layer was present. We then used energy dispersive spectroscopy (EDS)-scanning transmission electron microscope (STEM) to perform Ce and nitrogen (N) (for S-layer immunocytochemistry) elemental analysis on the phagocytosed cells. That is, the elemental mapping and analysis of N by EDS appeared to reflect the presence of the same moieties detected by the 3,3'-diaminobenzidine-tetrahydrochloride (DAB) reaction with horseradish peroxidase (HRP)-conjugated secondary antibodies, instead of immunogold labeling. We focused on the use of EDS-STEM to visualize the presence of N resulting from the DAB reaction. In a parallel set of experiments, we used EDS-STEM to perform Ce and gold (Au; from immunogold labeling of the S-layer) elemental analysis on the same phagocytosing cells.

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Section: Discussionmentioning

confidence: 99%