Role of Lipid Droplets in the Development of Oocytes and Preimplantation Embryos in Mammals

Brusentsev, E. Yu.; Mokrousova, Valentina; Igonina, T. N.; Рожкова, И. Н.; Amstislavsky, S. Ya.

doi:10.1134/s1062360419050102

Cited by 12 publications

(10 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We speculate that this pathway is conserved across organisms. Indeed, LD accumulation, composition, and localization are dynamic during oocyte maturation (Ami et al ., 2011; Dunning et al ., 2014), LDs are hubs for FA trafficking, and FA levels (including that of AA) are critical for oocyte development in many species (Dunning et al ., 2014; Prates et al ., 2014; Brusentsev et al ., 2019). Further, in both mouse models and human patients, metabolic syndrome causes failures in oocyte maturation and decreased fertility (Wattanakumtornkul et al ., 2003; Jungheim et al ., 2010; Cardozo et al ., 2011; Marei et al ., 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Since both triglycerides and LDs are abundant in oocytes, LDs might perform similar regulatory roles during follicle development. Indeed, LD accumulation, composition, and localization are dynamic during oocyte maturation across organisms (Ami et al ., 2011; Dunning et al ., 2014; Brusentsev et al ., 2019), and, in the context of obesity, oocytes display changes in LDs that are associated with infertility (Jungheim et al ., 2010; Cardozo et al ., 2011; Marei et al ., 2020). However, the functions of LDs in oogenesis remain largely undefined.…”

Section: Introductionmentioning

confidence: 99%

“…Fertility requires the production of high-quality oocytes. It is becoming increasingly clear that among the key factors ensuring oocyte quality are the amount and types of lipids present during oogenesis (Dunning et al ., 2014; Brusentsev et al ., 2019). In mammalian follicles, for example, fatty acids (FAs) likely contribute a critical source of energy since inhibitors of FA oxidation impair oocyte maturation (Dunning et al ., 2010; Dunning et al ., 2011).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Adipose triglyceride lipase promotes prostaglandin-dependent actin remodeling by regulating substrate release from lipid droplets

Giedt

Thomalla

Johnson

et al. 2021

Preprint

View full text Add to dashboard Cite

To ensure fertility, it is paramount to understand the factors controlling oocyte quality. One incompletely characterized factor contributing to oocyte quality is lipids. In somatic cells, a key regulator of lipid metabolism is lipid droplets (LDs), the sites of intracellular fat storage. Yet the role of LDs in fertility is poorly understood. Here we use Drosophila oogenesis as a model for uncovering if and how LDs promote egg development. LD accumulation in nurse cells coincides with dynamic actin remodeling necessary for late-stage follicle morphogenesis and fertility. Loss of major LD proteins, including PLIN2, Jabba, and ATGL, disrupts both actin bundle formation and cortical actin integrity; this unusual phenotype is also seen when Pxt, the enzyme responsible for prostaglandin (PG) synthesis, is missing. Further, both pharmacologic and genetic loss of PG synthesis or loss of PLIN2 or Jabba impairs intracellular LD dispersal. These similar phenotypes suggest that PGs and LD proteins act in the same pathway. Dominant genetic interaction studies indicate that there are three actin regulatory pathways: PLIN2 regulates actin remodeling independent of PG signaling, whereas Jabba and ATGL act in two separate PG-dependent pathways to regulate actin remodeling. We find that neither Jabba nor ATGL modulate the levels of Pxt or its localization to the endoplasmic reticulum. As ATGL is a triglyceride lipase, we hypothesize that it may release arachidonic acid (AA), the substrate for PG production, from triglycerides stored in LDs. Indeed, lipidomic analysis reveals the presence of AA-containing triglycerides in ovaries. In addition, exogenous AA is toxic and reduction of ATGL ameliorates toxicity; these observations suggest that ATGL indeed generates free AA. Our studies provide the first evidence that LDs and their associated proteins regulate PG signaling to control actin remodeling. In particular, we propose that ATGL releases AA from LDs to drive PG synthesis necessary for follicle development. We speculate that the same pathways are conserved across organisms to regulate oocyte development and promote fertility.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Adipose triglyceride lipase promotes prostaglandin-dependent actin remodeling by regulating substrate release from lipid droplets

Giedt

Thomalla

Johnson

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Approximately 24 h after attaining an LH peak, the MII oocyte generated from an ovulatory follicle, the LD, and the mitochondria reach a more central location [ 32 ]. A close spatial distribution of the endoplasmic reticulum (ER), mitochondria, and LD suggests a functional relationship between them in cattle oocytes [ 36 ]. LD may exhibit a dynamic distribution and relation to other organelles in the cell, which might change in accordance with the amount of FA they are submitted to [ 37 ].…”

Section: Oocytesmentioning

confidence: 99%

“…The lipid profile of the LD is also very important. It has been demonstrated that high-quality oocytes exhibit high levels of oleic acid, whereas in low-quality oocytes, there is a high level of stearic acid [ 36 ]. Therefore, it is necessary to improve the quality of oocytes and embryos by altering their lipid profiles by manipulating the diet or the composition of the maturation/cultivation media needs to be considered.…”

Section: Oocytesmentioning

confidence: 99%

Lipid Metabolism in Bovine Oocytes and Early Embryos under In Vivo, In Vitro, and Stress Conditions

Sterza

Poehland

2021

IJMS

View full text Add to dashboard Cite

Lipids are a potential reservoir of energy for initial embryonic development before activation of the embryonic genome and are involved in plasma membrane biosynthesis. Excessive lipid droplet formation is detrimental to cryotolerance and is related to alterations in mitochondrial function, which likely affects lipid metabolism. Increased lipid accumulation in in vitro produced embryos is a consequence of the stress during in vitro embryonic development process. There are several open questions concerning embryo lipid metabolism and developmental potential. Oocyte maturation and embryo development in vivo and in vitro may vary if the donors are subjected to any type of stress before follicle puncture because crucial changes in oocyte/embryonic metabolism occur in response to stress. However, little is known about lipid metabolism under additional stress (such as heat stress). Therefore, in this review, we aimed to update the information regarding the energy metabolism of oocytes and early bovine embryos exhibiting developmental competence, focusing on lipid metabolic pathways observed under in vivo, in vitro, and stress conditions.

show abstract

The Vast Utility of Drosophila Oogenesis

Giedt

Tootle

2023

Methods in Molecular Biology

View full text Add to dashboard Cite

Role of Lipid Droplets in the Development of Oocytes and Preimplantation Embryos in Mammals

Cited by 12 publications

References 68 publications

Adipose triglyceride lipase promotes prostaglandin-dependent actin remodeling by regulating substrate release from lipid droplets

Adipose triglyceride lipase promotes prostaglandin-dependent actin remodeling by regulating substrate release from lipid droplets

Lipid Metabolism in Bovine Oocytes and Early Embryos under In Vivo, In Vitro, and Stress Conditions

The Vast Utility of Drosophila Oogenesis

Contact Info

Product

Resources

About