Role of liposome on recognition and folding of oxidized and fragmented superoxide dismutase for its re-activation

Umakoshi, Hiroshi; Tuan, Le Quoc; Shimanocuhi, Toshinori; Kuboi, Ryoichi

doi:10.1016/j.bej.2009.06.005

Cited by 8 publications

(2 citation statements)

References 24 publications

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“…Liposomes, a closed phospholipid bilayer membrane, have a nano-order interface harboring hydration layer and non-polar (hydrophobic) layer on its surface [11]. Liposomes are composed of amphiphiles, which hydrophilic compounds can be trapped within the liposome interior and lipophilic or amphiphilic compounds normally are incorporated into the liposome membrane [12].…”

Section: Introductionmentioning

confidence: 99%

Optimization on condition of glycyrrhetinic acid liposome by RSM and the research of its immunological activity

Zhao

Liu

et al. 2012

International Journal of Biological Macromolecules

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Section: Introductionmentioning

confidence: 99%

Optimization on condition of glycyrrhetinic acid liposome by RSM and the research of its immunological activity

Zhao

Liu

et al. 2012

International Journal of Biological Macromolecules

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“…Cell entry of liposomes functioning as drug vectors has been widely studied. SODs can be encapsulated in the lipid bilayer of the liposome, facilitating entry of SOD into the cells, reducing enzyme degradation and enabling slow release (15)(16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

Effect of superoxide dismutase-entrapped liposomes and protein transduction domain-superoxide dismutase on human umbilical vein endothelial cells

Zhang

Cao

Xie

et al. 2014

Molecular Medicine Reports

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Superoxide dismutases (SOD) are able to remove the superoxide anion free radicals produced by environmental stress and thereby protect cells from being injured by reactive oxygen species. However, SOD is unable to transduce automatically across cell membranes. Protein transduction domains (PTDs) are peptides able to mediate protein delivery into cells and were first observed in the HIV‑1 Tat protein. In the present study, PTD (RKKRRQRRR) was fused to Dunaliella salina (Ds)MnSOD to form PTD‑DsMnSOD. This was inserted into pET32a to construct the recombinant plasmid pET32a‑PTD‑DsMnSOD and transduced into E. coli BL21(DE3) to obtain purified PTD‑DsMnSOD proteins. Liposome‑encapsulated proteins are also able to cross cell membranes. In this study, DsMnSOD proteins were purified and encapsulated by liposomes. The obtained MnSOD, PTD‑MnSOD and liposome MnSOD were used to protect human umbilical vein endothelial cells (HUVECs) from injury under oxygen pressure. A cell counting kit 8 was used to test the survival rate of HUVECs and results indicated that the protective effect of MnSOD was limited compared with that of PTD‑MnSOD and liposome MnSOD. Thus, PTD and liposomes exhibited improved effects when MnSOD was present in cells.

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Development of LIPOzymes Based on Biomembrane Process Chemistry

Umakoshi

Shimanouchi

Kuboi

2010

Pharmaceutical Process Chemistry

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Role of liposome on recognition and folding of oxidized and fragmented superoxide dismutase for its re-activation

Cited by 8 publications

References 24 publications

Optimization on condition of glycyrrhetinic acid liposome by RSM and the research of its immunological activity

Optimization on condition of glycyrrhetinic acid liposome by RSM and the research of its immunological activity

Effect of superoxide dismutase-entrapped liposomes and protein transduction domain-superoxide dismutase on human umbilical vein endothelial cells

Development of LIPOzymes Based on Biomembrane Process Chemistry

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